##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580110_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9814350
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49716578275688	34.0	31.0	34.0	31.0	34.0
2	32.69372826524426	34.0	31.0	34.0	31.0	34.0
3	32.774431826865765	34.0	31.0	34.0	31.0	34.0
4	36.2602728657527	37.0	37.0	37.0	35.0	37.0
5	36.186667379907995	37.0	37.0	37.0	35.0	37.0
6	36.21775767116518	37.0	37.0	37.0	35.0	37.0
7	36.180435892341315	37.0	37.0	37.0	35.0	37.0
8	36.17143774167418	37.0	37.0	37.0	35.0	37.0
9	37.95553592443718	39.0	38.0	39.0	35.0	39.0
10-11	37.90282937739127	39.0	38.0	39.0	35.0	39.0
12-13	37.8656357782227	39.0	38.0	39.0	35.0	39.0
14-15	39.2592298012604	41.0	39.0	41.0	36.0	41.0
16-17	39.24681461329584	41.0	39.0	41.0	36.0	41.0
18-19	39.246379790816505	41.0	39.0	41.0	36.0	41.0
20-21	39.22842664058241	41.0	39.0	41.0	36.0	41.0
22-23	39.15643506701921	40.5	39.0	41.0	36.0	41.0
24-25	39.076918746529316	40.0	39.0	41.0	35.0	41.0
26-27	38.939520701829466	40.0	38.0	41.0	35.0	41.0
28-29	38.82572844864917	40.0	38.0	41.0	35.0	41.0
30-31	38.70564662968001	40.0	38.0	41.0	35.0	41.0
32-33	38.553887012384926	40.0	38.0	41.0	34.0	41.0
34-35	38.51759408417267	40.0	38.0	41.0	34.5	41.0
36-37	38.5620451176084	40.0	38.0	41.0	35.0	41.0
38-39	38.46531624610901	40.0	38.0	41.0	34.0	41.0
40-41	38.3318366473582	40.0	38.0	41.0	34.0	41.0
42-43	38.29402334336966	40.0	38.0	41.0	34.0	41.0
44-45	38.1929958682949	40.0	38.0	41.0	33.5	41.0
46-47	37.98179084707597	40.0	37.5	41.0	33.0	41.0
48-49	37.75873664582983	40.0	37.0	41.0	33.0	41.0
50-51	37.58022706547046	40.0	37.0	41.0	33.0	41.0
52-53	37.398384661235845	40.0	36.0	41.0	32.0	41.0
54-55	37.19546663813701	39.5	36.0	41.0	32.0	41.0
56-57	36.97502483608186	39.0	35.0	41.0	31.5	41.0
58-59	36.74036996846454	39.0	35.0	41.0	31.0	41.0
60-61	36.4769186446377	38.5	35.0	40.0	31.0	41.0
62-63	36.18767845043227	38.0	35.0	40.0	31.0	41.0
64-65	35.88776877735153	37.0	35.0	40.0	30.5	41.0
66-67	35.55515260817069	37.0	35.0	39.5	30.0	41.0
68-69	35.201004702298164	36.0	34.5	39.0	29.5	41.0
70-71	34.87386082623913	36.0	34.0	39.0	29.0	40.5
72-73	34.52326012420588	35.0	34.0	38.0	29.0	40.0
74-75	34.17186828470556	35.0	34.0	37.0	29.0	39.5
76-77	32.952606744206186	34.5	32.0	36.0	27.0	39.0
78-79	33.58941427603458	35.0	33.5	36.0	29.0	39.0
80-81	33.528623240459126	35.0	34.0	36.0	29.0	37.5
82-83	33.32340771421439	35.0	34.0	35.5	29.0	37.0
84-85	33.1054295495881	35.0	33.5	35.0	29.0	37.0
86-87	32.92670074941285	35.0	33.0	35.0	29.0	36.0
88-89	32.7604318166766	35.0	33.0	35.0	27.5	36.0
90-91	32.598668684120696	35.0	33.0	35.0	27.0	36.0
92-93	32.47728525067885	35.0	33.0	35.0	27.0	35.5
94-95	32.371826356304794	35.0	33.0	35.0	27.0	35.0
96-97	32.269868101300645	35.0	33.0	35.0	26.5	35.0
98-99	32.18802401585434	35.0	33.0	35.0	26.0	35.0
100	30.911810563104027	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.13468324538655452
1101	2	0.049107113137395686
1101	3	0.04781058205903577
1101	4	-0.013174789283908694
1101	5	-0.008009493295396908
1101	6	0.015253749250376814
1101	7	-0.03950982314804463
1101	8	-0.011331429230992285
1101	9	-0.021959508257822336
1101	10-11	-0.02381144555192094
1101	12-13	-0.0670818943611664
1101	14-15	-0.06094787928298473
1101	16-17	-0.08303605651608592
1101	18-19	-0.06513997354798562
1101	20-21	-0.08415057933641634
1101	22-23	-0.07455846970749036
1101	24-25	-0.12219437883484119
1101	26-27	-0.11079469907939199
1101	28-29	-0.10060831631575695
1101	30-31	-0.14274541223442583
1101	32-33	-0.088207965056327
1101	34-35	-0.14740081831170215
1101	36-37	-0.06105329484975641
1101	38-39	-0.05155017419174612
1101	40-41	-0.02352580793793635
1101	42-43	-0.10616588521590131
1101	44-45	-0.15940857938320363
1101	46-47	-0.09461140002574808
1101	48-49	-0.12028893723663003
1101	50-51	-0.1199933195038625
1101	52-53	-0.12474724690324024
1101	54-55	-0.11765574124340361
1101	56-57	-0.12729243163099824
1101	58-59	-0.2096531943708655
1101	60-61	-0.20474897238235457
1101	62-63	-0.16616118181283213
1101	64-65	-0.15708026804007602
1101	66-67	-0.18052415589600201
1101	68-69	-0.18495796083735172
1101	70-71	-0.2254707585766127
1101	72-73	-0.2254769273832622
1101	74-75	-0.19435023347344327
1101	76-77	-0.2225934472601523
1101	78-79	-0.19300302256345248
1101	80-81	-0.1567304033695862
1101	82-83	-0.11364071311078305
1101	84-85	-0.12137275117959945
1101	86-87	-0.12099962411625853
1101	88-89	-0.13069693256758796
1101	90-91	-0.09516201603820917
1101	92-93	-0.2339292805627906
1101	94-95	-0.18533835726894665
1101	96-97	-0.1246856648953667
1101	98-99	-0.16532126561485683
1101	100	-0.10255756063800803
1102	1	0.08794374653294312
1102	2	0.053143927724825346
1102	3	-0.005420411587707008
1102	4	0.01399345606294844
1102	5	0.028666781613807757
1102	6	0.01542025378712708
1102	7	0.011821994931949575
1102	8	0.01994763731603655
1102	9	0.00757691767079649
1102	10-11	0.023854154637668046
1102	12-13	-0.007430330648809047
1102	14-15	0.05567227573748568
1102	16-17	-0.02954757032548372
1102	18-19	-0.03546500757126836
1102	20-21	-0.0597961986092983
1102	22-23	-0.03074004991662349
1102	24-25	-0.0680903481240378
1102	26-27	-0.07302109205065932
1102	28-29	-0.07374631521376784
1102	30-31	-0.07786776235351311
1102	32-33	-0.08262834114933071
1102	34-35	-0.05271658113486666
1102	36-37	-0.04707604048640235
1102	38-39	-0.05973766764920185
1102	40-41	-0.02038155591551316
1102	42-43	-0.05351860373133377
1102	44-45	-0.01810509892749934
1102	46-47	-0.036628677157004574
1102	48-49	-0.05885046441706976
1102	50-51	-0.09128649984414494
1102	52-53	-0.060545352065069835
1102	54-55	-0.14558344894483355
1102	56-57	-0.16345324572524333
1102	58-59	-0.12728682837829552
1102	60-61	-0.0982959773680463
1102	62-63	-0.09572830973015556
1102	64-65	-0.1552420424414933
1102	66-67	-0.11161167190085308
1102	68-69	-0.14125470840805576
1102	70-71	-0.19382263627516494
1102	72-73	-0.19311525557760234
1102	74-75	-0.19848029577241277
1102	76-77	-0.10508247228425205
1102	78-79	-0.07502314082055506
1102	80-81	-0.12964269037227893
1102	82-83	-0.06963402426158183
1102	84-85	-0.14693821971891197
1102	86-87	-0.15147646973684203
1102	88-89	-0.12310229867993883
1102	90-91	-0.14298190698455926
1102	92-93	-0.1476951744808659
1102	94-95	-0.20300045010769452
1102	96-97	-0.19784261842163176
1102	98-99	-0.17004105905987643
1102	100	-0.08896479213894537
1103	1	0.11492487682239272
1103	2	0.07804387589729345
1103	3	0.062343352067394164
1103	4	0.025392155813683814
1103	5	-0.006144183256743929
1103	6	0.043939587065850105
1103	7	0.025809951625511474
1103	8	0.013267629776031242
1103	9	0.024488177859446125
1103	10-11	-0.012196652802934693
1103	12-13	0.006130515346676191
1103	14-15	0.014669034800654401
1103	16-17	0.03722338317503926
1103	18-19	0.04743359089821553
1103	20-21	0.04860629949767059
1103	22-23	-0.0556934249684744
1103	24-25	-0.03993148099468158
1103	26-27	0.0013089043432614744
1103	28-29	0.013662221295447807
1103	30-31	-0.0685752475758008
1103	32-33	-0.10913220286012404
1103	34-35	-0.11418887586099657
1103	36-37	-0.04945963385258523
1103	38-39	-0.0663268358515623
1103	40-41	-0.06912023450160376
1103	42-43	-0.06338409056962746
1103	44-45	-0.03780877147922013
1103	46-47	-0.09191754257765439
1103	48-49	-0.057729124365572204
1103	50-51	-0.07875260221550207
1103	52-53	-0.0930201739004346
1103	54-55	-0.001059988479035212
1103	56-57	-0.0265643561927007
1103	58-59	-0.057148465039119856
1103	60-61	-0.04786612973612847
1103	62-63	-0.020216331520344966
1103	64-65	-0.09519128470225979
1103	66-67	-0.06432565138019442
1103	68-69	-0.07359348532227727
1103	70-71	-0.04728964930668411
1103	72-73	-0.12661741302236607
1103	74-75	-0.1992920726559717
1103	76-77	-0.20553747808900624
1103	78-79	-0.09439848100894466
1103	80-81	-0.053377703796790854
1103	82-83	-0.014217972224408015
1103	84-85	-0.030511153349088715
1103	86-87	-0.06109245168415356
1103	88-89	-0.006044845506437468
1103	90-91	-0.008706553138793538
1103	92-93	-0.05825854691394028
1103	94-95	-0.08419516349331957
1103	96-97	-0.07704575593847807
1103	98-99	-0.10280656887456274
1103	100	-0.09648997492110567
1104	1	0.1079342162243151
1104	2	0.0683677843172461
1104	3	0.019943048688695342
1104	4	0.03584683182497628
1104	5	0.0077967064567161515
1104	6	0.02529673966624557
1104	7	0.015429686193712655
1104	8	0.0034106736559920137
1104	9	0.026439920527636218
1104	10-11	-0.008714653965753882
1104	12-13	-0.006614070797134275
1104	14-15	0.03703849931308412
1104	16-17	0.0028759019830388866
1104	18-19	-0.018023142747715326
1104	20-21	-0.031786921804688006
1104	22-23	-0.12851186262374625
1104	24-25	-0.0996981937331185
1104	26-27	-0.09175558526619199
1104	28-29	-0.12604150824709137
1104	30-31	-0.10756160397456682
1104	32-33	-0.08164967521869926
1104	34-35	-0.06436074192441055
1104	36-37	-0.02201445199640517
1104	38-39	-0.04737150564639592
1104	40-41	0.005035985691556277
1104	42-43	-0.05606220922915384
1104	44-45	-0.05989452874585055
1104	46-47	-0.005349538858339997
1104	48-49	-0.07388556566743176
1104	50-51	-0.05999237863116491
1104	52-53	-0.10105468484032798
1104	54-55	-0.13210974160919164
1104	56-57	-0.1472156263079185
1104	58-59	-0.10685245819284006
1104	60-61	-0.1358125920422779
1104	62-63	-0.08095983141539875
1104	64-65	-0.11092888810525636
1104	66-67	-0.11542583157139319
1104	68-69	-0.10330851767574245
1104	70-71	-0.13415841746618895
1104	72-73	-0.12657174157787665
1104	74-75	-0.11838089127791562
1104	76-77	-0.1778696472905068
1104	78-79	-0.03530207750328884
1104	80-81	-0.027352926239409214
1104	82-83	-0.03768167292769675
1104	84-85	-0.01899076693693047
1104	86-87	0.005998062499919854
1104	88-89	0.03800167486389938
1104	90-91	0.05260593271604819
1104	92-93	-0.011758080109842695
1104	94-95	-0.05719465509734789
1104	96-97	-0.06772385871679631
1104	98-99	-0.10425131531957987
1104	100	-0.032536746060294774
1105	1	0.12388985661308283
1105	2	0.09799969961257915
1105	3	0.02507407886038493
1105	4	0.005440505743841584
1105	5	-0.021278533169038383
1105	6	-0.0022298629178791884
1105	7	0.015357104404415622
1105	8	0.024400855544008948
1105	9	0.022458225039798663
1105	10-11	0.020423264340756475
1105	12-13	0.005517037256460355
1105	14-15	0.07265320950532583
1105	16-17	0.044390143047813524
1105	18-19	0.01139435897089669
1105	20-21	-0.04120077475064221
1105	22-23	-0.013223630240567275
1105	24-25	-0.0020475668114983137
1105	26-27	0.0035742645718173094
1105	28-29	-0.01897965793163081
1105	30-31	-0.011543072900600748
1105	32-33	0.011378363116250512
1105	34-35	0.02939564646480619
1105	36-37	0.046547157061489486
1105	38-39	0.04283940073315762
1105	40-41	0.029073551934359898
1105	42-43	0.04232062749865406
1105	44-45	0.01210309504119067
1105	46-47	0.019120037679918767
1105	48-49	0.005664864779994616
1105	50-51	0.010327801067404607
1105	52-53	-0.03807448210771014
1105	54-55	0.014203930291337485
1105	56-57	-0.032416449610948916
1105	58-59	0.025630179663934882
1105	60-61	0.035244606518610055
1105	62-63	-0.010549765116749654
1105	64-65	0.009954313556200134
1105	66-67	-0.02362634253693585
1105	68-69	-0.030318086754270723
1105	70-71	-0.03119924253906703
1105	72-73	-0.01367376181304536
1105	74-75	-0.08346886097038464
1105	76-77	-0.08404758160848047
1105	78-79	-1.1258075250708544E-4
1105	80-81	-0.007145043793535422
1105	82-83	0.010441703894123577
1105	84-85	0.076854510995922
1105	86-87	0.021198965048320417
1105	88-89	0.08240445585771283
1105	90-91	0.08935222170808998
1105	92-93	0.07163801135465775
1105	94-95	-0.006064090699815949
1105	96-97	-0.03724293126833089
1105	98-99	-0.04918877601020455
1105	100	-0.03834804597666519
1106	1	0.09731310618975186
1106	2	0.028564691524813668
1106	3	-0.005121929155407656
1106	4	0.008865020572677906
1106	5	-0.004983661228465053
1106	6	0.005329768719484207
1106	7	0.00712599511163603
1106	8	0.04241674317143662
1106	9	6.041309263338235E-5
1106	10-11	0.004483147892095474
1106	12-13	-0.022155633675701836
1106	14-15	0.013538082923290062
1106	16-17	-0.03316505641881662
1106	18-19	-0.044270553768370746
1106	20-21	-0.07234997980103941
1106	22-23	-0.04151907798451049
1106	24-25	-0.04778118956179611
1106	26-27	-0.06118368965938714
1106	28-29	-0.030994628819470904
1106	30-31	-0.0764398412041416
1106	32-33	-0.06176977366735059
1106	34-35	-0.04854302289904666
1106	36-37	-0.040080809557068164
1106	38-39	-0.029325491171121598
1106	40-41	-0.08365155119884804
1106	42-43	-0.018587429581643278
1106	44-45	-0.014763799673922051
1106	46-47	-0.005495405215157234
1106	48-49	0.01602003697171739
1106	50-51	-0.03814991875122331
1106	52-53	-0.0364838707333206
1106	54-55	-0.08744371592295153
1106	56-57	-0.05430300876906102
1106	58-59	-0.04642670364875556
1106	60-61	-0.03317213875279634
1106	62-63	-0.1083016014370628
1106	64-65	-0.02398938651084137
1106	66-67	-0.020957433267646763
1106	68-69	0.00175179759258981
1106	70-71	0.06826256313487278
1106	72-73	0.0034969373713593654
1106	74-75	-0.01655549286155633
1106	76-77	-0.06328108124291987
1106	78-79	-0.054009761967684256
1106	80-81	0.011581036690138546
1106	82-83	0.0586589029895066
1106	84-85	0.08973627810618012
1106	86-87	0.059074780927616644
1106	88-89	0.08034703462344339
1106	90-91	0.07433149657465066
1106	92-93	0.07538077877176619
1106	94-95	0.0013062228478517568
1106	96-97	-0.03919140444234159
1106	98-99	0.08081274683819828
1106	100	0.07515358862305632
1107	1	0.0449046158712747
1107	2	0.02120717440951836
1107	3	0.008964449942318709
1107	4	-0.004681839662808329
1107	5	-0.01812295266706343
1107	6	-0.017987556256088055
1107	7	0.01815956535486407
1107	8	-0.017320652741595666
1107	9	-0.021971975681061906
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2316	5	-0.04647971939540696
2316	6	-0.024387554026176872
2316	7	-0.0320177178190022
2316	8	0.0054692632767086025
2316	9	-0.017234703042028343
2316	10-11	-0.015370540025635648
2316	12-13	-3.413629235566873E-4
2316	14-15	0.044541984563288395
2316	16-17	0.021311055501982423
2316	18-19	0.043890018114886686
2316	20-21	0.03919275591381677
2316	22-23	0.03964077974368507
2316	24-25	0.02611443661704982
2316	26-27	0.04780525083972975
2316	28-29	0.05586794700853659
2316	30-31	-0.30348468152116936
2316	32-33	-0.10839865186139264
2316	34-35	-0.15985326327687943
2316	36-37	-0.4702274594941045
2316	38-39	-0.34843079584039316
2316	40-41	-0.24548750057655155
2316	42-43	-0.3794467410336395
2316	44-45	-0.19921739182731102
2316	46-47	-0.34254318370359016
2316	48-49	-0.28049782680756863
2316	50-51	-0.19366353371562184
2316	52-53	-0.2978318925936847
2316	54-55	-0.13793942808000992
2316	56-57	-0.8819295837256362
2316	58-59	-0.7307154879191629
2316	60-61	-0.3587125933655031
2316	62-63	-0.12534386240773188
2316	64-65	-0.04426499211550805
2316	66-67	0.0325950070340042
2316	68-69	-0.0855152812568889
2316	70-71	-0.08879262951806055
2316	72-73	0.05617894264423029
2316	74-75	0.012544063616694245
2316	76-77	0.0119977829126654
2316	78-79	-0.1433371723575263
2316	80-81	-0.13854709880592964
2316	82-83	-0.24875622148667276
2316	84-85	-0.12769566221701467
2316	86-87	-0.16358008179267358
2316	88-89	-0.14778586578978548
2316	90-91	-0.08840399926216946
2316	92-93	-0.11097268022024309
2316	94-95	-0.08667950337095931
2316	96-97	-0.274200907481287
2316	98-99	-0.008292457639655026
2316	100	-0.3019589461381216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	16.0
13	104.0
14	366.0
15	1292.0
16	3011.0
17	6559.0
18	12369.0
19	19158.0
20	28485.0
21	42150.0
22	55413.0
23	59498.0
24	58345.0
25	58916.0
26	65585.0
27	79079.0
28	97792.0
29	120215.0
30	147376.0
31	184299.0
32	233637.0
33	304049.0
34	445790.0
35	680473.0
36	1226641.0
37	2485690.0
38	2821548.0
39	576408.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09498929313416	19.382029624186504	13.70447127838884	23.818509804290496
2	16.0365805259737	19.917838197820263	39.71724708035515	24.328334195850886
3	19.218775966936764	24.88462847009979	29.49098155390327	26.40561400906017
4	13.247253501372446	16.49095399605246	37.46084636552481	32.80094613705028
5	13.964630374812218	37.25808931636869	34.26052494313933	14.516755365679765
6	31.924376041204972	36.33639517645081	17.336104785339835	14.403123997004386
7	28.004992285270646	31.87530739449396	21.722900860505895	18.3967994597295
8	26.297855395990304	34.94187974167506	19.656764319351772	19.103500542982864
9	26.2331324750605	15.85268916984434	20.416441691611347	37.49773666348381
10-11	25.59477621654227	25.751908495143716	27.430569088046397	21.22274620026762
12-13	25.939205618877555	23.311602725989495	27.78157522389804	22.967616431234912
14-15	23.313744609916938	24.822285191434556	25.35921999690249	26.50475020174602
16-17	22.809973387406597	27.679303093122375	26.19548938524996	23.31523413422107
18-19	22.515946825320203	27.018977237962222	28.19155193350083	22.27352400321675
20-21	23.474657797595057	26.17053195834397	28.03755182281665	22.317258421244322
22-23	23.488949937917337	25.8820975524167	27.80672636574219	22.822226143923775
24-25	23.1250108259844	26.03033313464468	28.05094071436213	22.793715325008787
26-27	23.038567562726243	26.230269058341655	27.741290792869155	22.989872586062944
28-29	22.74911353376245	26.42889420607002	27.966884404333868	22.85510785583366
30-31	23.30365359080798	26.15385038642289	27.849103967815736	22.693392054953392
32-33	23.2237543008665	26.28721296921271	27.535863546633347	22.953169183287443
34-35	23.28530087276302	26.322074739794665	27.782282736943824	22.61034165049849
36-37	23.244016368317304	26.137473932814736	27.615028843065353	23.003480855802607
38-39	23.176881952591494	26.22408028033919	27.783988005844222	22.815049761225094
40-41	23.171935565625912	25.850912889595712	27.9742424330434	23.00290911173498
42-43	23.04715585702943	26.00769718940193	27.67876721876493	23.26637973480371
44-45	23.07879947482335	26.36206040387411	27.55174722722531	23.007392894077235
46-47	23.108461589841493	26.253095843088552	27.207031511709907	23.43141105536005
48-49	23.356163856589536	26.24184566241543	27.209821600307876	23.19216888068716
50-51	23.252717288855422	26.34344676640843	27.06311691143579	23.340719033300353
52-53	23.083980630777027	26.527294452672923	26.965029354278226	23.423695562271824
54-55	22.9983848835576	26.436502681136094	27.02547681751803	23.539635617788278
56-57	23.324173931897906	26.377129998968858	26.798814671290653	23.499881397842582
58-59	22.955875113348732	26.49552440947979	27.13955459749692	23.409045879674554
60-61	23.06157030904801	26.597899099748822	26.924152079260942	23.416378511942224
62-63	23.106722714334698	26.444038346223586	26.802664285099294	23.646574654342427
64-65	22.80782901542156	26.392609079474955	26.935082153491756	23.86447975161173
66-67	23.019150937066364	26.28691461404547	26.87272788223251	23.821206566655658
68-69	22.805569587105612	26.34338484801475	26.985714820653868	23.865330744225773
70-71	22.81584304636676	26.09735842070236	26.996193243882892	24.090605289047986
72-73	22.991869844142293	26.274368050800483	26.895674378036453	23.83808772702077
74-75	23.020735355999054	26.514509124352475	26.699354635436812	23.765400884211658
76-77	22.88564658363227	26.40687976009794	26.597958733463045	24.109514922806742
78-79	22.79488285813108	26.389453759783223	26.649195025223428	24.166468356862275
80-81	22.674049838088315	26.69166148460293	26.68884926190872	23.94543941540004
82-83	23.01301414004529	26.294712066290852	26.988926900219834	23.70334689344402
84-85	23.076215702536476	26.387884320374226	26.571929027470066	23.96397094961923
86-87	22.586612161784046	26.65559701819517	26.82248748257832	23.935303337442463
88-89	22.777919437631045	26.167860387973647	26.793724092061527	24.26049608233378
90-91	22.73321139939194	26.230220186695362	26.77310490002349	24.263463513889207
92-93	22.790078540590482	26.306048983708337	26.746178302964463	24.157694172736722
94-95	22.374923298489975	26.20926515520962	26.94167669144279	24.474134854857613
96-97	22.464073943575148	26.16876733332749	26.856328176376522	24.51083054672084
98-99	22.26814464360388	26.335868995497925	26.759339793131048	24.636646567767148
100	22.463105214484756	26.150841379220395	26.557082425288286	24.828970981006556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	947.0
1	660.5
2	610.0
3	880.0
4	727.0
5	639.0
6	1104.5
7	2141.5
8	3450.5
9	4339.0
10	4152.5
11	3706.0
12	3969.5
13	4641.5
14	5385.5
15	6174.5
16	7010.5
17	7944.5
18	8974.0
19	9831.5
20	10723.0
21	11806.5
22	13291.5
23	15347.5
24	17913.0
25	21602.0
26	27162.5
27	34090.5
28	42956.5
29	55831.0
30	72190.5
31	89353.5
32	111818.5
33	136070.0
34	157664.0
35	179673.5
36	205376.0
37	238692.5
38	260272.5
39	269029.5
40	274072.5
41	276603.0
42	287417.5
43	306598.5
44	328521.0
45	360711.5
46	389234.0
47	409321.0
48	427138.0
49	431411.5
50	424814.0
51	422742.5
52	418863.5
53	406640.0
54	393213.0
55	362795.0
56	327241.0
57	286560.5
58	245185.5
59	216957.0
60	182338.0
61	145349.0
62	115958.0
63	90430.5
64	65609.5
65	44880.5
66	31970.0
67	23986.0
68	18613.5
69	13943.5
70	10378.5
71	7944.0
72	5962.0
73	4403.0
74	3240.0
75	2179.0
76	1444.5
77	826.5
78	455.5
79	264.5
80	164.0
81	91.5
82	63.0
83	48.5
84	26.5
85	16.0
86	15.5
87	9.0
88	7.0
89	5.0
90	2.5
91	2.0
92	0.0
93	1.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3577516595597263
2	5.807822219505113E-4
3	0.0019053732544692212
4	0.0012940235471528934
5	0.004574933643083852
6	0.0
7	7.132413252023822E-5
8	4.483231186986403E-4
9	0.0030465593747930324
10-11	7.641871341454096E-5
12-13	5.604038983733004E-5
14-15	3.056748536581638E-4
16-17	1.1717536056896279E-4
18-19	9.424974654460051E-4
20-21	1.9868865487780648E-4
22-23	1.6302658861768737E-4
24-25	0.0
26-27	0.0019970757105666702
28-29	0.011090902606897043
30-31	0.0036782874056865713
32-33	0.013821597966243306
34-35	0.011111280930474254
36-37	0.012695695588602405
38-39	0.012609087713399256
40-41	0.014152745724372985
42-43	0.006500685221130283
44-45	0.0013093072898358016
46-47	0.0059657542272284975
48-49	0.012680411845919495
50-51	0.0034643150081258567
52-53	0.005435917814221013
54-55	0.005160810445928666
56-57	2.64918206503742E-4
58-59	5.960659646334194E-4
60-61	3.413369199182829E-4
62-63	8.660787520314642E-4
64-65	3.8718814796700747E-4
66-67	0.0013296856134130126
68-69	0.00160479298170536
70-71	9.017408182915833E-4
72-73	0.0027510736829234744
74-75	0.007636776760559792
76-77	0.0035152608170688836
78-79	0.0016353604670711764
80-81	4.992689276416676E-4
82-83	2.547290447151365E-4
84-85	0.0016149821434939655
86-87	0.0043609612455231365
88-89	0.0015538471727623326
90-91	1.9868865487780648E-4
92-93	0.001925751578046432
94-95	0.003382801713817013
96-97	0.0016710225333312957
98-99	0.0021804806227615683
100	0.002302750564224834
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9814350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.39269662235661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13422100946177	39.957090204671374
2	15.182380398092263	16.81985982793477
3	5.491569645385999	9.125785540422273
4	2.565104987266807	5.683523294566565
5	1.378890377663179	3.819022818269159
6	0.8097869042092025	2.691376818817062
7	0.5215083384497179	2.022142722444215
8	0.3666769122688784	1.6248978367785958
9	0.25412191896639086	1.266884852615674
>10	1.2194130312757772	11.565332449429501
>50	0.0528635892935271	1.9811735876708512
>100	0.022258842952803917	2.089667883890763
>500	6.984168544521802E-4	0.25339116649748855
>1k	4.093177965359382E-4	0.30943605193937396
>5k	2.40775156641966E-5	0.06979349018335809
>10k+	7.22325469925898E-5	0.7206214538691039
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23215	0.23654139092247578	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19723	0.2009608379566655	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11093	0.11302837172100037	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.037832357721092E-5
2	0.0	1.018916178860546E-5	0.0	0.0	2.037832357721092E-5
3	0.0	1.018916178860546E-5	0.0	1.018916178860546E-5	2.037832357721092E-5
4	0.0	1.018916178860546E-5	0.0	1.018916178860546E-5	2.037832357721092E-5
5	0.0	1.018916178860546E-5	0.0	1.018916178860546E-5	2.037832357721092E-5
6	6.113497073163276E-5	1.018916178860546E-5	0.0	1.018916178860546E-5	5.0945808943027304E-5
7	6.113497073163276E-5	1.018916178860546E-5	0.0	1.018916178860546E-5	6.113497073163276E-5
8	6.113497073163276E-5	1.018916178860546E-5	0.0	1.018916178860546E-5	6.113497073163276E-5
9	6.113497073163276E-5	1.018916178860546E-5	0.0	2.037832357721092E-5	8.151329430884368E-5
10-11	6.62295516259355E-5	1.018916178860546E-5	0.0	3.056748536581638E-5	8.660787520314641E-5
12-13	7.132413252023823E-5	1.528374268290819E-5	0.0	3.056748536581638E-5	1.2226994146326552E-4
14-15	8.151329430884368E-5	2.037832357721092E-5	0.0	3.056748536581638E-5	1.6302658861768737E-4
16-17	9.170245609744914E-5	2.037832357721092E-5	0.0	4.075664715442184E-5	1.7321575040629282E-4
18-19	9.170245609744914E-5	2.037832357721092E-5	0.0	4.075664715442184E-5	1.8340491219489828E-4
20-21	9.170245609744914E-5	2.037832357721092E-5	0.0	1.0189161788605461E-4	1.9868865487780648E-4
22-23	9.170245609744914E-5	2.037832357721092E-5	0.0	2.802019491866502E-4	2.496344638208338E-4
24-25	9.170245609744914E-5	2.037832357721092E-5	0.0	6.877684207308686E-4	3.056748536581638E-4
26-27	9.170245609744914E-5	2.5472904471513652E-5	0.0	0.0013908205841446455	3.515260817068884E-4
28-29	9.170245609744914E-5	3.056748536581638E-5	0.0	0.004243785884954174	3.617152434954938E-4
30-31	9.170245609744914E-5	3.056748536581638E-5	0.0	0.014692771299169073	3.76998986178402E-4
32-33	9.170245609744914E-5	3.056748536581638E-5	0.0	0.04006378415279667	4.0756647154421843E-4
34-35	9.170245609744914E-5	3.056748536581638E-5	0.0	0.08131460565396587	4.381339569100348E-4
36-37	9.170245609744914E-5	3.056748536581638E-5	0.0	0.14715696913193435	4.6870144227585117E-4
38-39	9.170245609744914E-5	3.056748536581638E-5	0.0	0.23878300651596895	4.7889060406445665E-4
40-41	9.170245609744914E-5	3.056748536581638E-5	0.0	0.33673141878983326	4.890797658530621E-4
42-43	9.170245609744914E-5	3.056748536581638E-5	0.0	0.4357395038897125	5.247418321131812E-4
44-45	9.170245609744914E-5	3.056748536581638E-5	0.0	0.5594257388415942	5.502147365846949E-4
46-47	9.170245609744914E-5	3.056748536581638E-5	0.0	0.7035514323414185	5.705930601619058E-4
48-49	1.0189161788605461E-4	3.056748536581638E-5	0.0	0.8558080769485499	6.011605455277222E-4
50-51	1.2736452235756826E-4	3.056748536581638E-5	0.0	1.0157473495442897	6.113497073163277E-4
52-53	1.3245910325187097E-4	3.056748536581638E-5	0.0	1.173169899178244	6.21538869104933E-4
54-55	1.3245910325187097E-4	3.056748536581638E-5	0.0	1.348341968647949	6.368226117878413E-4
56-57	1.3245910325187097E-4	3.056748536581638E-5	0.0	1.5530218506574558	6.521063544707495E-4
58-59	1.3245910325187097E-4	3.056748536581638E-5	0.0	1.7782838394799452	6.62295516259355E-4
60-61	1.4264826504047643E-4	3.056748536581638E-5	0.0	2.011442428688604	6.724846780479604E-4
62-63	1.8340491219489828E-4	3.056748536581638E-5	0.0	2.2482283594940062	7.030521634137768E-4
64-65	1.8340491219489828E-4	3.056748536581638E-5	0.0	2.4926561616408627	7.030521634137768E-4
66-67	2.0378323577210922E-4	3.056748536581638E-5	0.0	2.7647984838527258	7.132413252023823E-4
68-69	2.0887781666641196E-4	3.056748536581638E-5	0.0	3.067192427414959	7.234304869909876E-4
70-71	2.2416155934932013E-4	3.056748536581638E-5	0.0	3.3929704972820414	7.234304869909876E-4
72-73	2.6491820650374195E-4	3.056748536581638E-5	0.0	3.7212143443019663	7.743762959340149E-4
74-75	2.6491820650374195E-4	3.056748536581638E-5	0.0	4.046279172843846	7.743762959340149E-4
76-77	2.6491820650374195E-4	3.056748536581638E-5	0.0	4.402283391156827	7.794708768283176E-4
78-79	2.6491820650374195E-4	3.056748536581638E-5	0.0	4.7976636252018725	7.845654577226204E-4
80-81	2.6491820650374195E-4	3.056748536581638E-5	0.0	5.21605098656559	8.202275239827396E-4
82-83	2.9548569186955834E-4	3.056748536581638E-5	0.0	5.644011065429702	8.86457075608675E-4
84-85	2.9548569186955834E-4	3.056748536581638E-5	0.0	6.069036665698697	8.86457075608675E-4
86-87	2.9548569186955834E-4	3.056748536581638E-5	0.0	6.510991558279459	8.86457075608675E-4
88	3.1586401544676925E-4	3.056748536581638E-5	0.0	6.862196681390006	8.966462373972805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14555	0.0	52.11901	1
GTATCAA	26350	0.0	40.623394	1
CAACGCA	31540	0.0	33.125107	5
ATCAACG	31700	0.0	32.972908	3
TATCAAC	33395	0.0	31.945896	2
AACGCAG	34500	0.0	30.825932	6
TCAACGC	34170	0.0	30.754337	4
GTGGTAT	5910	0.0	28.656904	1
ACGCAGA	38615	0.0	26.932468	7
CGCAGAG	40185	0.0	25.728205	8
GTACTAG	4370	0.0	25.69318	1
GTACATG	35485	0.0	24.462189	1
TGGTATC	6805	0.0	23.826576	2
TACATGG	35850	0.0	23.714836	2
GCAGAGT	44440	0.0	22.958134	9
TAGTACT	6330	0.0	22.496683	4
ACATGGG	36050	0.0	22.436617	3
CTAGTAC	6530	0.0	22.023685	3
GAGTACT	23835	0.0	21.204784	12-13
CTGTGCG	6630	0.0	20.20143	9
>>END_MODULE