FastQCFastQC Report
Thu 26 May 2016
ERR580109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6814334
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149550.21946385369428617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120510.1768478034683947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69090.101389218667591No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97650.049.7453041
GTATCAA176650.041.341081
ATCAACG206100.034.575093
CAACGCA206150.034.5672155
TATCAAC217450.033.469022
AACGCAG227100.032.2139936
TCAACGC224400.031.8190084
ACGCAGA243200.029.1939687
CGCAGAG251150.028.1584228
GTACATG244050.027.6886621
GTACTAG31850.027.3140641
TACATGG249600.026.6339952
GTGGTAT41150.025.5977761
ACATGGG252700.025.4276033
GCAGAGT278850.024.6221529
CATGGGG207200.023.888584
ATGGGGG150450.022.6826065
GCATCGT32200.022.3275228
GAGTACT146900.022.02604912-13
CAGAGTA270000.020.93986310-11
Produced by FastQC (version 0.11.5)