FastQCFastQC Report
Thu 26 May 2016
ERR580109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6814334
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134870.19792102940654216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114440.1679401097744842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101000.049.1554641
GTATCAA175450.039.739011
CAACGCA206450.032.9424135
ATCAACG208150.032.6507873
TATCAAC221750.031.2087672
AACGCAG228650.030.5036456
TCAACGC224400.030.3280354
GTACTAG34000.028.0702551
ACGCAGA250800.027.1537537
GTACATG244800.026.3686811
CGCAGAG258250.026.2056678
TACATGG251150.025.2528972
ACATGGG250200.024.2704013
GCAGAGT288950.023.0308769
TAGTACT46400.022.3858134
CATGGGG200350.022.2156584
CTGTGCG45700.022.0073369
CTAGTAC45850.021.8344383
TCTAACG14350.021.6234252
GAGTACT147950.021.5216412-13
Produced by FastQC (version 0.11.5)