FastQCFastQC Report
Thu 26 May 2016
ERR580108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17184002
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516760.3007215664895756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408930.2379713410182331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259550.15104164908733134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA264700.047.050141
GTATCAA468250.040.789851
CAACGCA529650.034.573285
ATCAACG543000.033.7020033
GTACTAG128550.033.1842581
TATCAAC593100.032.0056342
AACGCAG605750.031.0221396
GTACATG669500.030.0318761
TCAACGC630550.029.3643234
TACATGG683750.028.986812
ACATGGG672150.028.3669013
ACGCAGA650300.028.108347
CGCAGAG667350.027.3331458
GCATCGT138500.027.2159968
TAGGCAT165950.027.061995
CATGGGG556200.026.4828594
CTGTGCG180450.026.0729839
CTAGGCA178400.025.9929854
TCTAACG63100.025.7899152
GGGCACC158100.025.71432194
Produced by FastQC (version 0.11.5)