FastQCFastQC Report
Thu 26 May 2016
ERR580108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17184002
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451680.26284913141886274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372990.2170565389831775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251810.14653745966742787No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266950.047.4088061
GTATCAA474150.040.423961
CAACGCA548250.033.639875
ATCAACG564800.032.679393
TATCAAC613550.031.0185832
AACGCAG620750.030.4965976
GTACTAG128750.030.4531841
TCAACGC640350.029.1464984
GTACATG674800.028.4597361
TACATGG683350.027.6851482
ACGCAGA676350.027.2738747
ACATGGG670800.027.1650473
CGCAGAG696750.026.421448
GGGCACC167450.026.13562894
TAGTACT187450.025.8008424
CTGTGCG185200.025.7829519
CTAACGC57250.025.6966553
TCTAACG59550.025.6522662
CATGGGG551150.025.6088144
TAGGCAT163350.025.435315
Produced by FastQC (version 0.11.5)