FastQCFastQC Report
Thu 26 May 2016
ERR580107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062846
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61650.12176945536166812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.043.676681
GTATCAA108500.039.175821
CAACGCA125150.032.6437235
ATCAACG128800.031.7230383
TATCAAC134400.031.1371422
AACGCAG140700.029.7592966
TCAACGC138800.029.7067554
GTACATG204800.026.3796791
ACGCAGA158550.025.8153537
TACATGG209850.025.5437052
GTACTAG22300.024.4586541
CGCAGAG166350.024.2939158
ACATGGG213850.024.1854553
CATGGGG169150.022.2641224
TAGTACT32150.021.6434174
CTAGTAC31200.021.3995483
GCAGAGT187700.021.084339
ATGGGGG121100.020.53635
TATACCG9650.020.4612775
CTGTGCG33450.020.093339
Produced by FastQC (version 0.11.5)