FastQCFastQC Report
Thu 26 May 2016
ERR580107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062846
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56010.1106294759903817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65800.044.734351
GTATCAA113300.039.469431
CAACGCA131950.033.089495
ATCAACG131900.032.9604843
TATCAAC139800.031.6342562
TCAACGC142800.030.5430284
AACGCAG147450.030.087446
ACGCAGA169600.025.7146227
GTACATG208000.025.5362721
TACATGG211600.024.942462
CGCAGAG176550.024.5958758
ACATGGG217300.023.4674913
GTACTAG23450.021.322861
GCAGAGT202000.021.1711149
CATGGGG170350.021.0235084
GTGGTAT35600.020.8032931
ATGGGGG120600.020.3815945
TGGTATC34000.020.1812972
TGGGGGG106450.018.6746626
AGAGTAC178450.018.4073310-11
Produced by FastQC (version 0.11.5)