##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580107_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5062846
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4767421327846	34.0	31.0	34.0	31.0	34.0
2	32.68266662663648	34.0	31.0	34.0	31.0	34.0
3	32.76505684747274	34.0	31.0	34.0	31.0	34.0
4	36.24892046884302	37.0	37.0	37.0	35.0	37.0
5	36.171137340539296	37.0	35.0	37.0	35.0	37.0
6	36.199565027259375	37.0	37.0	37.0	35.0	37.0
7	36.17061214186645	37.0	37.0	37.0	35.0	37.0
8	36.15982255830021	37.0	37.0	37.0	35.0	37.0
9	37.93899735445242	39.0	38.0	39.0	35.0	39.0
10-11	37.885260582684126	39.0	38.0	39.0	35.0	39.0
12-13	37.84458849824782	39.0	38.0	39.0	35.0	39.0
14-15	39.23265432130465	41.0	39.0	41.0	36.0	41.0
16-17	39.20891352413247	41.0	39.0	41.0	36.0	41.0
18-19	39.198328173521375	41.0	39.0	41.0	36.0	41.0
20-21	39.178265347198	40.5	39.0	41.0	36.0	41.0
22-23	39.104787011100086	40.0	39.0	41.0	35.0	41.0
24-25	39.02197627579429	40.0	38.5	41.0	35.0	41.0
26-27	38.88373268710919	40.0	38.0	41.0	35.0	41.0
28-29	38.76903583478541	40.0	38.0	41.0	35.0	41.0
30-31	38.667785273342304	40.0	38.0	41.0	35.0	41.0
32-33	38.53098810431919	40.0	38.0	41.0	34.0	41.0
34-35	38.50963884739927	40.0	38.0	41.0	34.0	41.0
36-37	38.57447500872039	40.0	38.0	41.0	34.5	41.0
38-39	38.49050178496442	40.0	38.0	41.0	34.0	41.0
40-41	38.37617419135403	40.0	38.0	41.0	34.0	41.0
42-43	38.36067391739745	40.0	38.0	41.0	34.0	41.0
44-45	38.295006306729455	40.0	38.0	41.0	34.0	41.0
46-47	38.1295879827275	40.0	37.5	41.0	33.0	41.0
48-49	37.94413033696857	40.0	37.0	41.0	33.0	41.0
50-51	37.78264191721415	40.0	37.0	41.0	33.0	41.0
52-53	37.60663389721907	40.0	36.5	41.0	33.0	41.0
54-55	37.40239501655788	39.5	36.0	41.0	32.5	41.0
56-57	37.18185522530213	39.0	35.5	41.0	32.0	41.0
58-59	36.94674359046276	39.0	35.0	41.0	32.0	41.0
60-61	36.67129505815504	38.5	35.0	40.0	31.0	41.0
62-63	36.36265551035919	38.0	35.0	40.0	31.0	41.0
64-65	36.05407245252967	37.0	35.0	40.0	31.0	41.0
66-67	35.713238166833435	37.0	35.0	39.5	30.5	41.0
68-69	35.340844951633926	36.0	34.5	39.0	30.0	41.0
70-71	34.99181201640342	35.5	34.0	39.0	30.0	40.0
72-73	34.62306082389233	35.0	34.0	37.5	29.5	40.0
74-75	34.25896422684001	35.0	34.0	37.0	29.0	39.0
76-77	33.03397515942614	34.5	32.0	36.0	27.5	38.5
78-79	33.67826791492374	35.0	33.5	36.0	29.0	37.5
80-81	33.621204061905104	35.0	34.0	36.0	29.0	37.0
82-83	33.423044074419806	35.0	34.0	35.0	29.0	37.0
84-85	33.2209237650128	35.0	33.5	35.0	29.0	36.5
86-87	33.066859430446826	35.0	33.0	35.0	29.0	36.0
88-89	32.92165088568761	35.0	33.0	35.0	29.0	36.0
90-91	32.775038782534565	35.0	33.0	35.0	29.0	35.5
92-93	32.66545170443659	35.0	33.0	35.0	28.0	35.0
94-95	32.57208504860705	35.0	33.0	35.0	27.0	35.0
96-97	32.48132996737408	35.0	33.0	35.0	27.0	35.0
98-99	32.40625519717566	35.0	33.0	35.0	27.0	35.0
100	31.093381469631904	34.0	30.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.16596553748334486
1101	2	0.0961363056764526
1101	3	0.05377964334660845
1101	4	0.06514518539559333
1101	5	0.0363541136573744
1101	6	0.05210415928945622
1101	7	0.028199891375201958
1101	8	0.029870409423097044
1101	9	-0.0017655416246853406
1101	10-11	0.028954012403488605
1101	12-13	-0.032752518674122655
1101	14-15	0.06169601931252089
1101	16-17	0.0066168237527008955
1101	18-19	-0.028237955091022116
1101	20-21	0.023148353740175764
1101	22-23	-0.03678590648061686
1101	24-25	-0.052406500860399774
1101	26-27	-0.0711190122252745
1101	28-29	-0.15451077032780347
1101	30-31	-0.10960828530498645
1101	32-33	-0.11578312973822591
1101	34-35	-0.17035334402119418
1101	36-37	-0.03643762023732222
1101	38-39	-0.008149799548746728
1101	40-41	-0.1004071835736724
1101	42-43	-0.11362990545163854
1101	44-45	-0.12586727221181349
1101	46-47	-0.10045096875776238
1101	48-49	-0.16621056134651724
1101	50-51	-0.18694418824865267
1101	52-53	-0.09417963207792468
1101	54-55	-0.06531033965845978
1101	56-57	-0.071783600474852
1101	58-59	-0.15992331612285682
1101	60-61	-0.22535933468363822
1101	62-63	-0.1832725433867921
1101	64-65	-0.18140260082995496
1101	66-67	-0.1828206443037601
1101	68-69	-0.23148845040392985
1101	70-71	-0.21325757776232734
1101	72-73	-0.1871026148592918
1101	74-75	-0.17678059428106252
1101	76-77	-0.2170080553454028
1101	78-79	-0.1569965403584348
1101	80-81	-0.12255068369800881
1101	82-83	-0.05289181266149967
1101	84-85	-0.0733598778418525
1101	86-87	-0.07012486043033306
1101	88-89	-0.1972506333323878
1101	90-91	-0.14686063369449442
1101	92-93	-0.20912163804549522
1101	94-95	-0.10882224864010226
1101	96-97	-0.11605483247253545
1101	98-99	-0.19512871134154608
1101	100	-0.16972747469537453
1102	1	0.17956715811798318
1102	2	0.10939084901467311
1102	3	0.0884204736044083
1102	4	0.03134955262813577
1102	5	0.0732407566290334
1102	6	0.11085435965448198
1102	7	0.06300920461598736
1102	8	0.05236094768684296
1102	9	0.05367080735417318
1102	10-11	0.06117825427905643
1102	12-13	0.07079508864412531
1102	14-15	0.10716051933270876
1102	16-17	0.07844381575338133
1102	18-19	0.0875498292460648
1102	20-21	0.014353024704540474
1102	22-23	0.0646032061247297
1102	24-25	0.040951060474810674
1102	26-27	-0.019053772980605288
1102	28-29	0.05360639412593571
1102	30-31	0.07512834784927946
1102	32-33	0.033384409912500246
1102	34-35	0.013650550800591077
1102	36-37	0.10515610180220136
1102	38-39	0.09690600284606887
1102	40-41	0.12072242055990046
1102	42-43	0.06346463901528665
1102	44-45	0.04102925086220921
1102	46-47	0.11206297841170709
1102	48-49	0.0559024088646396
1102	50-51	0.02014479498652122
1102	52-53	0.020452442865050102
1102	54-55	-0.009977847368354276
1102	56-57	-0.01916078798085863
1102	58-59	0.035000482461008176
1102	60-61	0.05636030085384647
1102	62-63	0.056434220191881934
1102	64-65	-0.03477741413285429
1102	66-67	-0.03237825400091765
1102	68-69	-0.04965886394558794
1102	70-71	1.6625197854125418E-4
1102	72-73	-0.11436975738555333
1102	74-75	-0.09055847735253764
1102	76-77	0.018346603489291624
1102	78-79	0.08688622229219334
1102	80-81	0.0662874852419435
1102	82-83	0.040339572594689344
1102	84-85	0.008781846955173478
1102	86-87	0.046169803334613846
1102	88-89	0.058618053405666615
1102	90-91	0.1031481647306478
1102	92-93	0.07234784004133132
1102	94-95	0.018142612135115144
1102	96-97	-0.03323950886881022
1102	98-99	-0.05272110558311027
1102	100	0.16235025086665544
1103	1	0.08873977555501966
1103	2	0.08893157110683347
1103	3	0.020074650515816472
1103	4	0.028785329404641402
1103	5	0.035491108577396346
1103	6	0.034959783384628906
1103	7	-0.008422391915900107
1103	8	-0.022670521790708165
1103	9	-0.024396241259800888
1103	10-11	-0.06500572354133283
1103	12-13	-0.07471095231912983
1103	14-15	-0.03400940016032905
1103	16-17	-0.024788815251802987
1103	18-19	-0.028727243327843155
1103	20-21	-0.03518287402844322
1103	22-23	-0.06228741342203392
1103	24-25	-0.06116886470429961
1103	26-27	-0.07417712156830447
1103	28-29	-0.02448496735541994
1103	30-31	-0.07372149666601047
1103	32-33	-0.08715035552715733
1103	34-35	-0.10355433216432886
1103	36-37	-0.10056251989350073
1103	38-39	-0.056393488544429715
1103	40-41	-0.06468848650612102
1103	42-43	-0.06791013599264062
1103	44-45	-0.02194284031889282
1103	46-47	0.02215438695022698
1103	48-49	-0.05701497836483327
1103	50-51	-0.08198277698152623
1103	52-53	-0.0794391295892467
1103	54-55	-0.06904635083292732
1103	56-57	-0.034103005706505485
1103	58-59	-0.10622316062609372
1103	60-61	-0.14209485170939473
1103	62-63	-0.12000700345119952
1103	64-65	-0.06841877581029365
1103	66-67	-0.11089920816332466
1103	68-69	-0.13064247628868486
1103	70-71	-0.1191899276072732
1103	72-73	-0.17103952198210237
1103	74-75	-0.2143609705978733
1103	76-77	-0.14737617492244937
1103	78-79	-0.03734056970338173
1103	80-81	-0.07530771335889597
1103	82-83	-0.06564119671149626
1103	84-85	-0.09675540859267073
1103	86-87	-0.07415849880374736
1103	88-89	-0.049972231643096165
1103	90-91	-0.08509617174374995
1103	92-93	-0.11081883250748348
1103	94-95	-0.04077177912166974
1103	96-97	-0.14992891983077783
1103	98-99	-0.1412719018295121
1103	100	-0.004349747956915451
1104	1	0.10951253770821268
1104	2	0.05879900128324067
1104	3	0.03300746592856996
1104	4	0.026635849057335292
1104	5	0.010964099714485087
1104	6	0.02626944009286092
1104	7	0.05886048468322258
1104	8	0.038914602041216995
1104	9	0.03257031850169767
1104	10-11	0.026914301216990566
1104	12-13	-0.027446199616747435
1104	14-15	0.0116093222928626
1104	16-17	0.026341013744669795
1104	18-19	0.017712615132417397
1104	20-21	-0.06067762841436064
1104	22-23	-0.11700712386920031
1104	24-25	-0.002922932706105996
1104	26-27	-0.02355747297862365
1104	28-29	-0.14734960915152584
1104	30-31	-0.0633731773065378
1104	32-33	-0.027828456176521854
1104	34-35	-0.04319537086379199
1104	36-37	-0.03605554088937879
1104	38-39	0.025249335700181064
1104	40-41	0.06490968530427921
1104	42-43	0.11101653892423968
1104	44-45	0.08392121434885524
1104	46-47	0.07461976996231812
1104	48-49	0.07611714238802847
1104	50-51	0.015566872946543242
1104	52-53	0.02522470587368275
1104	54-55	0.020706246088302294
1104	56-57	0.09356048039396825
1104	58-59	0.06858772920507761
1104	60-61	-4.251522493134985E-4
1104	62-63	0.014128201738841994
1104	64-65	-0.022487256988632964
1104	66-67	-0.015497025242346751
1104	68-69	-0.0160990462396029
1104	70-71	-0.033195776648177855
1104	72-73	-0.005882905206640032
1104	74-75	-0.01538275033692571
1104	76-77	-0.09457251240735332
1104	78-79	0.0020920978726763906
1104	80-81	0.04440867795314318
1104	82-83	0.04518424592613712
1104	84-85	0.11634000800960109
1104	86-87	0.07194271583481537
1104	88-89	0.12131843830832167
1104	90-91	0.05911025967018446
1104	92-93	-0.012481544718333737
1104	94-95	0.03186311179555901
1104	96-97	-0.05257814956517137
1104	98-99	0.03856022011217419
1104	100	0.07022444976536235
1105	1	0.07468695339763087
1105	2	0.049537753964948195
1105	3	-0.015991082980214344
1105	4	-0.010475050780307527
1105	5	-0.00645254951342622
1105	6	0.012602432667698338
1105	7	-0.011441583849702397
1105	8	-0.058086425679562126
1105	9	-0.03520684222849013
1105	10-11	-0.01940586999467797
1105	12-13	-0.02150226163787039
1105	14-15	0.02115620096441262
1105	16-17	-0.05902475736771606
1105	18-19	-0.062278835779558506
1105	20-21	-0.06764265074038178
1105	22-23	-0.022360990240386514
1105	24-25	-0.04575986482685579
1105	26-27	-0.04234860137883345
1105	28-29	-0.05215136582877733
1105	30-31	-0.09251536511224145
1105	32-33	-0.038785624292628995
1105	34-35	-0.04194581168560063
1105	36-37	-0.0818459885540932
1105	38-39	-0.06154215314724354
1105	40-41	-0.03339592160118343
1105	42-43	0.022961839886697533
1105	44-45	0.029802382630435886
1105	46-47	-0.02739963065918971
1105	48-49	-0.06712627224659684
1105	50-51	-0.04859324230580597
1105	52-53	-0.12065592544288961
1105	54-55	-0.15221951668372924
1105	56-57	-0.048782288876772384
1105	58-59	-0.04895415765820843
1105	60-61	-0.042053062268799124
1105	62-63	-0.03313630941917722
1105	64-65	0.02210061593640944
1105	66-67	-0.055707612331907796
1105	68-69	-0.015397437167223416
1105	70-71	-0.005489232867340377
1105	72-73	-0.07639093007311004
1105	74-75	-0.061411618029730164
1105	76-77	-0.09008132948628855
1105	78-79	0.023820103464373688
1105	80-81	-0.020407045108441935
1105	82-83	0.04853600421807869
1105	84-85	0.029878166520610705
1105	86-87	-0.06213996225625351
1105	88-89	-0.032756732860626414
1105	90-91	-0.04791944046964858
1105	92-93	0.08731202248620917
1105	94-95	0.018518226655778847
1105	96-97	-0.024880983764816733
1105	98-99	-0.05497309134424455
1105	100	0.02500897062202867
1106	1	0.08743964843147722
1106	2	0.05565948820687794
1106	3	0.006388067827728605
1106	4	-0.004103071535297431
1106	5	-5.904045779629996E-4
1106	6	0.004709003308349224
1106	7	-0.014816320803760163
1106	8	-0.005094729194688341
1106	9	-0.007560846984212333
1106	10-11	-0.0021057659158429942
1106	12-13	0.0014291147096443524
1106	14-15	-0.010559922137289846
1106	16-17	-0.0031882143041528366
1106	18-19	-0.047000481896361634
1106	20-21	-0.03643715963266203
1106	22-23	-0.05177814881245979
1106	24-25	-0.07363506139908793
1106	26-27	-0.09693859157871287
1106	28-29	-0.08142706836085267
1106	30-31	-0.1756635976714449
1106	32-33	-0.09448808040099266
1106	34-35	-0.08179444156761662
1106	36-37	-0.09766179182231127
1106	38-39	-0.09125943099213174
1106	40-41	-0.09283575720221648
1106	42-43	-0.10856424439435841
1106	44-45	-0.06863377602519449
1106	46-47	-0.09518487881501869
1106	48-49	-0.05834380459080535
1106	50-51	-0.034609226382507075
1106	52-53	-0.07291846238327082
1106	54-55	-0.19938785089072297
1106	56-57	-0.13907507704977462
1106	58-59	-0.09040282856400239
1106	60-61	-0.10909878098247816
1106	62-63	-0.13184451645247464
1106	64-65	-0.14415512929078034
1106	66-67	-0.09579847238767769
1106	68-69	-0.09398139535336725
1106	70-71	-0.06019420358092731
1106	72-73	-0.10258156905633342
1106	74-75	-0.12766367871451934
1106	76-77	-0.14076517295780633
1106	78-79	-0.060646359602841926
1106	80-81	-0.03841781004982181
1106	82-83	-0.020554521569920325
1106	84-85	0.0039984725275559185
1106	86-87	-0.018443910414362108
1106	88-89	0.022257245609495158
1106	90-91	-0.026250684629452792
1106	92-93	-0.05797974009252016
1106	94-95	-0.03631494334216967
1106	96-97	-0.07909837936312414
1106	98-99	-0.020221399113687255
1106	100	-0.025309723302651577
1107	1	0.05574005170270624
1107	2	0.014156270297732476
1107	3	0.015290899737976815
1107	4	0.028393305031414684
1107	5	0.04437516341867109
1107	6	0.02287963885479627
1107	7	-0.027043474399782497
1107	8	-0.009839737117431469
1107	9	-0.029689473233638353
1107	10-11	-0.018256365565896715
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2316	7	0.008516440872334385
2316	8	0.03504346894170851
2316	9	0.034686909261147036
2316	10-11	0.013936222308011281
2316	12-13	0.019788101161367422
2316	14-15	0.10740369187973897
2316	16-17	0.053908338602731476
2316	18-19	-1.9261589281427405E-4
2316	20-21	0.041785986768751116
2316	22-23	0.02662849956118407
2316	24-25	0.0849337947147859
2316	26-27	0.07811457254408083
2316	28-29	0.11359672687152056
2316	30-31	-0.26477609981515116
2316	32-33	-0.07002415197248268
2316	34-35	-0.1536048800287162
2316	36-37	-0.30515621816428506
2316	38-39	-0.2964242826470809
2316	40-41	-0.18876520040807776
2316	42-43	-0.25693545001946205
2316	44-45	-0.12178360738631255
2316	46-47	-0.28194987052598464
2316	48-49	-0.29905673261915666
2316	50-51	-0.359482175562178
2316	52-53	-0.33057523937978317
2316	54-55	-0.12453426659985922
2316	56-57	-0.8915248426703073
2316	58-59	-0.7517204270319624
2316	60-61	-0.3495032999329126
2316	62-63	-0.10586312389545327
2316	64-65	-0.047876689343375745
2316	66-67	0.012688136541612494
2316	68-69	-0.08139571822536595
2316	70-71	-0.10008423102047459
2316	72-73	0.030727111558775277
2316	74-75	-0.032269806880073304
2316	76-77	-0.07150132456131075
2316	78-79	-0.14423000734765168
2316	80-81	-0.128585305619481
2316	82-83	-0.274935416785155
2316	84-85	-0.1137884279469219
2316	86-87	-0.20066229776930555
2316	88-89	-0.1674031185361713
2316	90-91	-0.07282267056668701
2316	92-93	-0.07514482041035819
2316	94-95	0.04281942469075517
2316	96-97	-0.2105005116446037
2316	98-99	-0.018033738335091698
2316	100	-0.2659488360906259
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	37.0
14	165.0
15	538.0
16	1209.0
17	2654.0
18	4645.0
19	7293.0
20	10487.0
21	14944.0
22	19124.0
23	21890.0
24	23180.0
25	26889.0
26	32508.0
27	40694.0
28	50957.0
29	63178.0
30	78315.0
31	97078.0
32	122894.0
33	162203.0
34	243600.0
35	359397.0
36	646493.0
37	1340675.0
38	1480522.0
39	211245.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.685807982621974	18.72706551825511	13.458272549708763	24.128853949414147
2	16.410495255510714	20.25364910195324	38.597039081571324	24.73881656096473
3	19.65500472471802	24.9105226897077	29.336429946179297	26.098042639394986
4	13.896850599463969	16.800753263083294	37.23463406319027	32.06776207426246
5	14.609708016797626	37.166012855003935	33.17685309187733	15.047426036321115
6	31.566514170093264	35.190246750543075	18.62213466496907	14.621104414394592
7	28.635604810260794	31.767777162401213	21.134840417757292	18.4617776095807
8	27.44674614533464	33.54152404210613	19.802339641931205	19.209390170628023
9	27.003619421145448	16.249194104001667	19.827530355415938	36.91965611943695
10-11	26.0819271235261	25.254387803211674	27.380555699853105	21.283129373409118
12-13	26.71822134770286	22.614194451360298	27.226492932974732	23.44109126796211
14-15	24.19342366415493	24.208988067850136	24.989292065541548	26.608296202453392
16-17	23.935350094156675	26.862858341507273	25.55430373241253	23.64748783192352
18-19	23.471721509966446	26.542312259569233	27.067981120283925	22.9179851101804
20-21	24.657262321500774	25.82197491248484	26.65498612734323	22.865776638671164
22-23	24.456668159900893	25.452276155103583	26.465078070867794	23.625977614127734
24-25	24.02613075728553	25.495195785137447	26.67357450730281	23.805098950274214
26-27	23.869832249072832	25.797166516813807	26.433925950038162	23.899075284075195
28-29	23.688843340735787	26.153599381781273	26.519609915362345	23.637947362120592
30-31	24.55354828119829	25.62137425719771	26.628056601364165	23.19702086023984
32-33	24.28817387870477	25.727595257584124	26.294612506441243	23.689618357269858
34-35	24.34226043614495	25.95135336732868	26.463120636584993	23.24326555994139
36-37	24.22511645180535	25.77760964643691	26.233628594795128	23.763645306962612
38-39	24.132638588184722	25.956305094709958	26.29913089033367	23.611925426771645
40-41	24.437023519037112	25.30991017188083	26.53298662692568	23.72007968215638
42-43	24.02605104985727	25.498183069444742	26.35473251817301	24.12103336252498
44-45	23.94136351013059	25.729390892682407	26.523755892506617	23.805489704680387
46-47	23.872864916628256	25.470779321788594	26.092927922593745	24.56342783898941
48-49	24.03633746355433	25.5890644277535	26.328136505405748	24.046461603286424
50-51	24.08043674261058	25.625128205786616	26.30238067467082	23.992054376931975
52-53	23.992534999105196	25.73959646487013	26.05582000245726	24.21204853356742
54-55	23.75175219228353	25.53291682028544	26.43476807754297	24.280562909888058
56-57	24.15512290836146	25.58849909645521	25.953689601304564	24.302688393878768
58-59	23.751989504659264	25.80885783372027	26.43345069880055	24.005701962819916
60-61	24.13263141989333	25.825608318671726	26.223735649693776	23.81802461174117
62-63	24.182923139035207	25.69711369430853	26.04815434292933	24.071808823726933
64-65	23.708198552075487	25.513985944233198	26.282606990987063	24.49520851270425
66-67	23.878615916476594	25.58468704647503	26.155698285870045	24.380998751178332
68-69	23.55153930290034	25.593497621111005	26.38601949034741	24.468943585641238
70-71	23.643117522000384	25.344544368102333	26.28714353166952	24.72519457822776
72-73	23.6085352252305	25.709631801207	26.10178316430507	24.580049809257424
74-75	23.741478074922718	25.71478885793883	26.02552733045706	24.51820573668139
76-77	23.52867743853064	25.535515932371183	25.968519146390207	24.96728748270797
78-79	23.417734832869506	25.575409329150244	26.094433961043624	24.912421876936627
80-81	23.473910919729235	25.88604153371229	25.915935899054027	24.724111647504447
82-83	23.804575046759883	25.685122767714958	26.246004337294522	24.26429784823064
84-85	23.792099668906054	25.769725520405114	25.859646783747014	24.57852802694181
86-87	23.208219878896337	26.02807250417522	26.115063292514428	24.64864432441401
88-89	23.3740531731126	25.46548916792611	26.215514030819715	24.944943628141576
90-91	23.6056634638517	25.522454007021356	26.076688608246997	24.79519392087995
92-93	23.537397430940413	25.580724695434643	26.16679974658029	24.715078127044656
94-95	23.118206241958944	25.673794531542963	26.233990846169807	24.974008380328282
96-97	23.099391034405997	25.68085565092833	26.153670360596397	25.066082954069273
98-99	22.878917045775967	25.645499752703277	26.06596343800911	25.409619763511643
100	23.01840192212426	25.650636849827325	25.97022714218331	25.3607340858651
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	110.0
1	91.0
2	192.0
3	280.0
4	184.5
5	169.5
6	305.0
7	526.5
8	815.5
9	984.5
10	912.5
11	828.5
12	907.5
13	1074.0
14	1252.5
15	1411.0
16	1567.0
17	1758.5
18	1991.5
19	2251.5
20	2618.0
21	2980.5
22	3298.5
23	3916.0
24	4660.5
25	5506.0
26	6847.0
27	8491.5
28	10491.5
29	13228.5
30	16805.0
31	21211.5
32	27146.5
33	34972.0
34	43939.0
35	54548.0
36	68595.0
37	85616.0
38	101641.0
39	117540.0
40	131687.0
41	142611.0
42	156077.0
43	172500.5
44	188811.5
45	208417.0
46	226346.0
47	240957.0
48	253403.5
49	259373.0
50	259406.5
51	256578.5
52	250309.5
53	240307.0
54	229722.0
55	210201.0
56	186922.5
57	162899.5
58	138180.0
59	116300.0
60	93317.0
61	72868.5
62	56802.0
63	43650.0
64	31747.0
65	21940.0
66	15550.0
67	11360.0
68	8512.0
69	6525.0
70	4960.0
71	3715.5
72	2779.0
73	2075.5
74	1531.5
75	1085.0
76	707.5
77	412.0
78	265.0
79	162.5
80	103.5
81	63.5
82	35.0
83	20.0
84	10.0
85	6.5
86	7.0
87	5.5
88	3.0
89	1.5
90	1.0
91	2.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3617135500467524
2	4.937934118478026E-4
3	0.0021726910121303315
4	0.0014418767625955836
5	0.0044638924431041355
6	0.0
7	3.9503472947824205E-5
8	3.9503472947824213E-4
9	0.0031207743628781127
10-11	9.875868236956053E-5
12-13	2.9627604710868154E-5
14-15	2.2714496944998918E-4
16-17	1.5801389179129682E-4
18-19	0.001145600715486902
20-21	1.7776562826520893E-4
22-23	1.9751736473912107E-4
24-25	0.0
26-27	0.0020146771203390346
28-29	0.011060972425390779
30-31	0.0034861814876454866
32-33	0.014665664331879737
34-35	0.011535014100764669
36-37	0.013154656491625462
38-39	0.012917635653938516
40-41	0.014497774571851484
42-43	0.006409438485784477
44-45	0.0011851041884347261
46-47	0.006113162438675796
48-49	0.013579318825814571
50-51	0.0036441953794367835
52-53	0.005688500104486686
54-55	0.005125575614980191
56-57	1.7776562826520893E-4
58-59	5.530486212695388E-4
60-61	3.6540712476737394E-4
62-63	8.295729319043083E-4
64-65	2.567725741608573E-4
66-67	0.0014517526308325396
68-69	0.0016492699955716608
70-71	7.209383812977918E-4
72-73	0.0026171050827933535
74-75	0.007693301356588765
76-77	0.0035849401700150467
78-79	0.0015900147861499243
80-81	3.7528299300432997E-4
82-83	2.468967059239013E-4
84-85	0.0016492699955716608
86-87	0.004760168490212817
88-89	0.0015900147861499243
90-91	1.5801389179129682E-4
92-93	0.0019455460426803424
94-95	0.0030911467581672437
96-97	0.0015900147861499243
98-99	0.0020146771203390346
100	0.0022516979580259797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5062846.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.28080546680177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89804953971863	50.852474182456575
2	13.756835399867217	17.961145911550876
3	4.0504294256580975	7.932458861801875
4	1.6170082250391717	4.222383975080024
5	0.8123706297393514	2.6516104523478914
6	0.4860302327498456	1.9037067045076228
7	0.303137078020823	1.3852322834036437
8	0.21112825560854406	1.102609806634125
9	0.14851458122307093	0.8725636337226141
>10	0.672157393919064	7.499049806231195
>50	0.029877256819362052	1.33175322405892
>100	0.013772694735690743	1.6465012202061788
>500	4.48036718953565E-4	0.19666031942228157
>1k	2.067858704475942E-4	0.315835070297547
>5k	3.446431174126415E-5	0.12601454827870917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5601	0.1106294759903817	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9503472947824205E-5
2	0.0	0.0	0.0	1.9751736473912103E-5	3.9503472947824205E-5
3	0.0	0.0	0.0	3.9503472947824205E-5	3.9503472947824205E-5
4	0.0	0.0	0.0	5.9255209421736315E-5	5.9255209421736315E-5
5	0.0	0.0	0.0	5.9255209421736315E-5	5.9255209421736315E-5
6	0.0	0.0	0.0	7.900694589564841E-5	7.900694589564841E-5
7	0.0	0.0	0.0	1.1851041884347263E-4	7.900694589564841E-5
8	0.0	0.0	0.0	1.1851041884347263E-4	7.900694589564841E-5
9	0.0	0.0	0.0	2.5677257416085737E-4	7.900694589564841E-5
10-11	0.0	0.0	0.0	3.7528299300432997E-4	7.900694589564841E-5
12-13	0.0	0.0	0.0	3.7528299300432997E-4	1.1851041884347263E-4
14-15	0.0	0.0	0.0	4.444140706630224E-4	1.5801389179129682E-4
16-17	0.0	0.0	0.0	5.234210165586707E-4	2.073932329760771E-4
18-19	0.0	0.0	0.0	6.518073036390994E-4	2.3702083768694526E-4
20-21	0.0	0.0	0.0	7.5056598600866E-4	2.3702083768694526E-4
22-23	0.0	0.0	0.0	8.888281413260447E-4	2.468967059239013E-4
24-25	0.0	0.0	0.0	0.0011554765837238582	2.6664844239781345E-4
26-27	0.0	0.0	0.0	0.0017677804144151333	2.9627604710868153E-4
28-29	0.0	0.0	0.0	0.003012139812271596	2.9627604710868153E-4
30-31	0.0	0.0	0.0	0.0077525565660105005	2.9627604710868153E-4
32-33	0.0	0.0	0.0	0.021252868445929423	3.5553125653041786E-4
34-35	0.0	0.0	0.0	0.043987117127402255	3.5553125653041786E-4
36-37	0.0	0.0	0.0	0.08306592774103735	3.5553125653041786E-4
38-39	0.0	0.0	0.0	0.14242977171338017	3.950347294782421E-4
40-41	0.0	0.0	0.0	0.2131212365535116	4.0491059771519816E-4
42-43	0.0	0.0	0.0	0.28423736372783215	4.147864659521542E-4
44-45	0.0	0.0	0.0	0.3776630772494364	4.444140706630224E-4
46-47	0.0	0.0	0.0	0.4849248821710161	5.135451483217147E-4
48-49	0.0	0.0	0.0	0.6004034094657431	6.320555671651873E-4
50-51	0.0	0.0	0.0	0.7268836539764394	6.518073036390994E-4
52-53	0.0	0.0	0.0	0.8558032379416636	6.715590401130116E-4
54-55	0.0	0.0	0.0	1.0069435254400392	7.110625130608357E-4
56-57	0.0	0.0	0.0	1.18022550952567	7.308142495347479E-4
58-59	0.0	0.0	0.0	1.3817129732960471	7.308142495347479E-4
60-61	0.0	0.0	0.0	1.584267030836016	7.308142495347479E-4
62-63	0.0	0.0	0.0	1.8001041311546904	7.308142495347479E-4
64-65	0.0	0.0	0.0	2.0250270302513647	7.308142495347479E-4
66-67	0.0	0.0	0.0	2.287576987330841	7.308142495347479E-4
68-69	0.0	0.0	0.0	2.57205137189636	7.308142495347479E-4
70-71	0.0	0.0	0.0	2.8755071752133086	7.60441854245616E-4
72-73	0.0	0.0	0.0	3.177195198115842	7.999453271934401E-4
74-75	0.0	0.0	0.0	3.4923835328982946	8.295729319043084E-4
76-77	0.0	0.0	0.0	3.8458507329671887	8.295729319043084E-4
78-79	0.0	0.0	0.0	4.233626699291268	8.295729319043084E-4
80-81	0.0	0.0	0.0	4.631229154511119	8.888281413260448E-4
82-83	0.0	0.0	0.0	5.051516479071258	0.0010468420331173414
84-85	0.0	0.0	0.0	5.4896099940626275	0.0010863455060651657
86-87	0.0	0.0	0.0	5.950190861029547	0.001106097242539078
88	0.0	0.0	0.0	6.308447857193365	0.0011258489790129899
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6580	0.0	44.73435	1
GTATCAA	11330	0.0	39.46943	1
CAACGCA	13195	0.0	33.08949	5
ATCAACG	13190	0.0	32.960484	3
TATCAAC	13980	0.0	31.634256	2
TCAACGC	14280	0.0	30.543028	4
AACGCAG	14745	0.0	30.08744	6
ACGCAGA	16960	0.0	25.714622	7
GTACATG	20800	0.0	25.536272	1
TACATGG	21160	0.0	24.94246	2
CGCAGAG	17655	0.0	24.595875	8
ACATGGG	21730	0.0	23.467491	3
GTACTAG	2345	0.0	21.32286	1
GCAGAGT	20200	0.0	21.171114	9
CATGGGG	17035	0.0	21.023508	4
GTGGTAT	3560	0.0	20.803293	1
ATGGGGG	12060	0.0	20.381594	5
TGGTATC	3400	0.0	20.181297	2
TGGGGGG	10645	0.0	18.674662	6
AGAGTAC	17845	0.0	18.40733	10-11
>>END_MODULE