FastQCFastQC Report
Thu 26 May 2016
ERR580106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308922
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408260.33167811121071367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341350.2773191673486923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189100.1536284006024248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238600.050.056781
GTATCAA430700.040.482241
GTGGTAT68850.035.6505471
CAACGCA489400.033.872925
ATCAACG502800.032.9739383
TATCAAC541250.031.8925842
AACGCAG556100.030.7090846
GTACTAG107800.029.0942211
TCAACGC594600.028.1591974
ACGCAGA589450.028.0858887
CGCAGAG598650.027.535858
TAGGCAT131050.026.8707085
GGGCACC113450.026.59212194
GCATCGT108000.026.5410428
GCAGAGT639900.025.42019
TGGTATC98000.024.5667152
AGGGCTA130500.024.281965
CTAGGCA149300.024.218084
CTGTGCG140700.024.1842299
TAGTACT140150.024.0546594
Produced by FastQC (version 0.11.5)