FastQCFastQC Report
Thu 26 May 2016
ERR580106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308922
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350490.28474467544761434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307680.2499650253694028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172020.13975228699962514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243950.050.2992061
GTATCAA431850.039.6285971
CAACGCA496550.033.2013785
GTGGTAT69750.032.5203741
ATCAACG510000.032.3085253
TATCAAC547500.031.1591472
AACGCAG563250.030.0696936
TCAACGC597150.027.8837244
ACGCAGA595100.027.5837967
CGCAGAG602600.027.16258
TAGGCAT136900.026.3986935
GGGCACC113650.025.51422194
GCAGAGT645500.025.160819
AGGGCTA130400.024.5791195
TATTCTC70700.024.0629395
CTAGGCA154300.023.878844
GCTATTG131850.023.8090328
GTACATG577300.023.6402261
GAGTACT328950.023.32920512-13
GGGCTAT142100.023.2821986
Produced by FastQC (version 0.11.5)