##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580106_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12308922
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.530479192247704	34.0	31.0	34.0	31.0	34.0
2	32.734109615773015	34.0	31.0	34.0	31.0	34.0
3	32.81021075606783	34.0	31.0	34.0	31.0	34.0
4	36.28334690885197	37.0	37.0	37.0	35.0	37.0
5	36.21789958535768	37.0	37.0	37.0	35.0	37.0
6	36.25050260290869	37.0	37.0	37.0	35.0	37.0
7	36.21688454927247	37.0	37.0	37.0	35.0	37.0
8	36.2149508299752	37.0	37.0	37.0	35.0	37.0
9	37.998325767276775	39.0	38.0	39.0	35.0	39.0
10-11	37.94983451840868	39.0	38.0	39.0	35.0	39.0
12-13	37.90239953588137	39.0	38.0	39.0	35.0	39.0
14-15	39.283381720998804	41.0	39.0	41.0	36.0	41.0
16-17	39.272422678444144	41.0	39.0	41.0	36.0	41.0
18-19	39.28373171103042	41.0	39.0	41.0	36.0	41.0
20-21	39.27103364535091	41.0	39.0	41.0	36.0	41.0
22-23	39.190759231393294	41.0	39.0	41.0	35.5	41.0
24-25	39.09816651693788	40.5	39.0	41.0	35.0	41.0
26-27	38.947535454363916	40.0	38.0	41.0	35.0	41.0
28-29	38.845914613806144	40.0	38.0	41.0	35.0	41.0
30-31	38.720010290096894	40.0	38.0	41.0	35.0	41.0
32-33	38.56189676886408	40.0	38.0	41.0	35.0	41.0
34-35	38.52255087813539	40.0	38.0	41.0	34.5	41.0
36-37	38.55561713690281	40.0	38.0	41.0	35.0	41.0
38-39	38.447879270012436	40.0	38.0	41.0	34.0	41.0
40-41	38.30230344298225	40.0	38.0	41.0	34.0	41.0
42-43	38.250104436440495	40.0	38.0	41.0	34.0	41.0
44-45	38.10554933242732	40.0	37.5	41.0	33.5	41.0
46-47	37.848388429141075	40.0	37.0	41.0	33.0	41.0
48-49	37.56918554687405	40.0	37.0	41.0	33.0	41.0
50-51	37.358802541766046	40.0	36.0	41.0	32.5	41.0
52-53	37.15297809995059	39.5	35.5	41.0	32.0	41.0
54-55	36.92748995403497	39.0	35.0	41.0	32.0	41.0
56-57	36.67277662495546	39.0	35.0	41.0	31.0	41.0
58-59	36.39745251452564	38.5	35.0	40.5	31.0	41.0
60-61	36.12537657643781	37.5	35.0	40.0	31.0	41.0
62-63	35.82325548086177	37.0	35.0	40.0	30.5	41.0
64-65	35.52844164582406	36.5	35.0	40.0	30.0	41.0
66-67	35.216833366886235	36.0	35.0	39.0	30.0	41.0
68-69	34.90174460444221	35.5	34.0	39.0	29.0	41.0
70-71	34.63207293863752	35.0	34.0	38.5	29.0	40.5
72-73	34.33159544759484	35.0	34.0	37.5	29.0	40.0
74-75	34.01347705347389	35.0	34.0	37.0	29.0	39.5
76-77	32.82575630912277	34.5	32.0	36.0	26.5	39.0
78-79	33.48645787177789	35.0	33.5	36.0	28.5	39.0
80-81	33.45898592906836	35.0	34.0	36.0	29.0	38.0
82-83	33.27189639352658	35.0	34.0	35.5	29.0	37.0
84-85	33.071712047570045	35.0	34.0	35.0	29.0	37.0
86-87	32.90953980372936	35.0	33.0	35.0	28.5	36.5
88-89	32.75955270494036	35.0	33.0	35.0	27.5	36.0
90-91	32.602171985491495	35.0	33.0	35.0	27.0	36.0
92-93	32.47928876306146	35.0	33.0	35.0	27.0	36.0
94-95	32.3879548915819	35.0	33.0	35.0	27.0	35.0
96-97	32.289307544559954	35.0	33.0	35.0	27.0	35.0
98-99	32.2180683247485	35.0	33.0	35.0	26.0	35.0
100	30.895651300739416	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.17454843758763872
1101	2	0.0990927587333914
1101	3	0.056486224594401335
1101	4	0.054782133479747586
1101	5	0.028809914393086444
1101	6	0.04752825995637977
1101	7	0.02862124308992975
1101	8	0.050327162095513245
1101	9	0.04576972717512717
1101	10-11	0.03650124074442829
1101	12-13	3.833192103641636E-4
1101	14-15	0.03189021998764474
1101	16-17	0.03670472678921044
1101	18-19	0.02699632808457153
1101	20-21	0.01960469670756737
1101	22-23	-0.022215233684136138
1101	24-25	-0.03428793311062606
1101	26-27	-0.0288891071644386
1101	28-29	0.0023326930714517857
1101	30-31	0.011851327930919808
1101	32-33	0.02049211746568602
1101	34-35	-0.04466033377823919
1101	36-37	0.024585909358805225
1101	38-39	0.04025230648245781
1101	40-41	0.08764897660368831
1101	42-43	0.05033395535753726
1101	44-45	-0.01384988887441807
1101	46-47	0.06639673141290814
1101	48-49	0.059749616240004855
1101	50-51	0.08466023197845374
1101	52-53	0.04161229007134892
1101	54-55	0.053560177236427364
1101	56-57	0.014684115793947683
1101	58-59	0.0030010429219586854
1101	60-61	-0.027851449282074725
1101	62-63	-0.016862107241315982
1101	64-65	-0.019283535592052203
1101	66-67	0.003457879942651232
1101	68-69	0.01547294811002331
1101	70-71	-0.02225211754563361
1101	72-73	-0.027860984843236736
1101	74-75	-0.01831809524292538
1101	76-77	-0.02768116635888873
1101	78-79	-0.013810771224385121
1101	80-81	0.032190954385320936
1101	82-83	0.014358641038043629
1101	84-85	0.01631525415211854
1101	86-87	0.03076446751527584
1101	88-89	0.03735241516689314
1101	90-91	-0.010038666800220142
1101	92-93	-0.05592676640352323
1101	94-95	-0.0551924954360814
1101	96-97	-0.030005838470387403
1101	98-99	-0.017271517948898918
1101	100	0.018437642871766968
1102	1	0.14971676921854993
1102	2	0.08296842456092435
1102	3	0.057748176299440956
1102	4	0.022156111537007916
1102	5	0.043091363864689924
1102	6	0.054723677133935666
1102	7	0.05275235026574876
1102	8	0.045164564198387325
1102	9	0.032990371746649316
1102	10-11	0.04412791501942337
1102	12-13	0.018156943330083664
1102	14-15	0.07991688015222564
1102	16-17	0.014211683302427502
1102	18-19	0.04844223522265878
1102	20-21	0.010726811980347861
1102	22-23	0.0028364201988111404
1102	24-25	0.025582041469590422
1102	26-27	0.03231757461909268
1102	28-29	0.02223248384336074
1102	30-31	0.04633697696077377
1102	32-33	-0.012274307072267732
1102	34-35	-0.013417104251473688
1102	36-37	0.03622961495393895
1102	38-39	0.029487246361675545
1102	40-41	0.05836853481162052
1102	42-43	0.07383751081891887
1102	44-45	0.061202487666569994
1102	46-47	0.0545244395415736
1102	48-49	0.026384265006051066
1102	50-51	0.04779171264075188
1102	52-53	0.05455132220945558
1102	54-55	0.006202371845745347
1102	56-57	-0.01080659083984159
1102	58-59	-0.002935568618887885
1102	60-61	0.013620763901982968
1102	62-63	-0.021800577678511956
1102	64-65	0.006812515975795463
1102	66-67	-0.030922134912096055
1102	68-69	-0.05454257949937613
1102	70-71	-0.04805753124698953
1102	72-73	-0.054292123405360826
1102	74-75	-0.08078209207990739
1102	76-77	-0.0415975943032052
1102	78-79	-0.007652095113222401
1102	80-81	-0.03513379885767165
1102	82-83	-0.016064554299717315
1102	84-85	-0.005950567540303098
1102	86-87	-0.06464738467796849
1102	88-89	-0.008456680695417162
1102	90-91	-0.04995483394096567
1102	92-93	-0.011202336607389896
1102	94-95	-0.07385224381334154
1102	96-97	-0.08700935032977952
1102	98-99	-0.1295988451155381
1102	100	0.06078183432266826
1103	1	0.10400715029854268
1103	2	0.05469405691204088
1103	3	0.02829987576130577
1103	4	0.026631406777013922
1103	5	-0.005369519688500191
1103	6	0.01119684337923843
1103	7	0.0037793162934107727
1103	8	-0.00839073430496029
1103	9	-0.016104560523174882
1103	10-11	-0.0011472928299909313
1103	12-13	-0.0014982281879198922
1103	14-15	0.019806314088690158
1103	16-17	-0.010274212189848697
1103	18-19	-0.012195568106228905
1103	20-21	-0.021103056404015774
1103	22-23	-0.09157445243219797
1103	24-25	-0.045085384099202486
1103	26-27	-0.019350605390663134
1103	28-29	-0.05195238993600526
1103	30-31	-0.12202249983607771
1103	32-33	-0.15272364780701508
1103	34-35	-0.09225736181038258
1103	36-37	-0.05043226807321588
1103	38-39	-0.0849846201393305
1103	40-41	-0.10716698887441112
1103	42-43	-0.08171683097845062
1103	44-45	-0.05765394912091182
1103	46-47	-0.06406627737200665
1103	48-49	-0.02511661476950877
1103	50-51	-0.03556400707760332
1103	52-53	-0.058370913417810755
1103	54-55	-0.01329748867984648
1103	56-57	-0.025143455058589836
1103	58-59	-0.10170784198136573
1103	60-61	-0.08876653221061304
1103	62-63	-0.0680949598637639
1103	64-65	-0.07519585104179072
1103	66-67	-0.03796310487398813
1103	68-69	-0.024026808960570634
1103	70-71	-0.004918118364038548
1103	72-73	0.0021276634209215217
1103	74-75	-0.08392481565091003
1103	76-77	-0.15031192832302054
1103	78-79	-0.04940257768349454
1103	80-81	-0.05143830942022731
1103	82-83	-0.008729910392531792
1103	84-85	-0.01948870689350457
1103	86-87	0.013259685018823575
1103	88-89	0.0035189441172036595
1103	90-91	-0.05298745773325919
1103	92-93	0.0011508210764290538
1103	94-95	8.200351498288683E-5
1103	96-97	-0.05163906805614005
1103	98-99	-0.005905127428903256
1103	100	0.03572948168388024
1104	1	0.11377550734614772
1104	2	0.06664464848965679
1104	3	0.023260569771736073
1104	4	0.02272187010510862
1104	5	-0.00945420760157134
1104	6	0.02888805988688148
1104	7	0.001400641062495822
1104	8	-0.028455693119916248
1104	9	-0.0015985847081338989
1104	10-11	-0.022878995658992096
1104	12-13	-0.04303552190166471
1104	14-15	0.011994591450111614
1104	16-17	-0.06943872421823727
1104	18-19	-0.09445630427367036
1104	20-21	-0.11014086693886327
1104	22-23	-0.11477209358602636
1104	24-25	-0.08967065666107743
1104	26-27	-0.08057961085160059
1104	28-29	-0.09519404120621289
1104	30-31	-0.11416198344335271
1104	32-33	-0.14650397757944944
1104	34-35	-0.1170209790323895
1104	36-37	-0.08485988829131941
1104	38-39	-0.03744319575115895
1104	40-41	-0.043522165215073016
1104	42-43	-0.050453194446639316
1104	44-45	-0.05265212685998932
1104	46-47	0.01159274661660703
1104	48-49	-0.04200206092486525
1104	50-51	-0.04719152461301945
1104	52-53	-0.05078003647057017
1104	54-55	-0.06252605407207312
1104	56-57	-0.040847871680334435
1104	58-59	-0.05531298156849829
1104	60-61	-0.06021676631354467
1104	62-63	-0.07210737395543987
1104	64-65	-0.07859375915765554
1104	66-67	-0.03186283086954944
1104	68-69	-0.013777005060511271
1104	70-71	-0.1107539130207087
1104	72-73	-0.1226414384332486
1104	74-75	-0.08161830065433406
1104	76-77	-0.13855862123587315
1104	78-79	-0.06917698080784618
1104	80-81	-0.06537568465701327
1104	82-83	-0.004054926773406464
1104	84-85	-0.02241255131558262
1104	86-87	-0.018824374595567406
1104	88-89	-0.01143083255441013
1104	90-91	0.00577258701354566
1104	92-93	-0.02220358644212439
1104	94-95	-0.084660528703985
1104	96-97	-0.05276387199676691
1104	98-99	-0.06261232952980578
1104	100	0.012347164202864747
1105	1	0.1131268179615077
1105	2	0.06255315823030116
1105	3	0.0351273150205742
1105	4	0.019439905035532945
1105	5	0.0055060343284552005
1105	6	0.03536509875606697
1105	7	0.023040431982387588
1105	8	0.006709699807046832
1105	9	0.048438484176870134
1105	10-11	0.033675852442314635
1105	12-13	0.009956526484351969
1105	14-15	0.06344056781748719
1105	16-17	0.01816529249013854
1105	18-19	0.022201022708671303
1105	20-21	0.051651515099322864
1105	22-23	0.013186444656760443
1105	24-25	-0.013708008957195261
1105	26-27	0.017651356321017886
1105	28-29	-0.005461188383534932
1105	30-31	-0.011853503332055482
1105	32-33	-0.02863555118743477
1105	34-35	-0.04419640084795162
1105	36-37	-0.040920956944447084
1105	38-39	0.01451420884718857
1105	40-41	0.06441627660352367
1105	42-43	0.06851293470508324
1105	44-45	0.08805089372274466
1105	46-47	0.04925096569983367
1105	48-49	0.00857059054710163
1105	50-51	0.026292435284311466
1105	52-53	-0.027316237409046096
1105	54-55	0.01207976294096369
1105	56-57	-0.0208247801159942
1105	58-59	-0.02398710065913434
1105	60-61	0.01648506909545233
1105	62-63	0.0021681402907987035
1105	64-65	0.022025848382369873
1105	66-67	-0.00821336532493433
1105	68-69	0.023121736658573866
1105	70-71	0.03993896273250641
1105	72-73	-0.03304797234757473
1105	74-75	-0.09227130561725261
1105	76-77	-0.06081058015413987
1105	78-79	0.04507917774778036
1105	80-81	0.045316772420690654
1105	82-83	0.08458821032993313
1105	84-85	0.056964073064854404
1105	86-87	0.025591879031622966
1105	88-89	0.09994796249748816
1105	90-91	0.10845140171098677
1105	92-93	0.07011062630299847
1105	94-95	0.022709738249950817
1105	96-97	-0.005466551197933711
1105	98-99	-0.015196684592396537
1105	100	0.07310342098621447
1106	1	0.1405169801138726
1106	2	0.07938680862134362
1106	3	0.035052504500292514
1106	4	0.04304775749800882
1106	5	0.04557401301009634
1106	6	0.026536626974206
1106	7	0.046921520245213344
1106	8	0.08442367601261935
1106	9	0.05350585893907578
1106	10-11	0.024210830701214547
1106	12-13	0.03234512222067565
1106	14-15	0.08487712230611777
1106	16-17	0.06350041116031235
1106	18-19	0.039678451219707256
1106	20-21	0.013144234095605611
1106	22-23	0.05122112095594389
1106	24-25	0.03423277674199454
1106	26-27	0.03303367506969579
1106	28-29	0.039275502741865864
1106	30-31	-0.0034856522283917
1106	32-33	-0.014226599591573574
1106	34-35	0.02789808612248379
1106	36-37	0.0767831678253117
1106	38-39	0.026136978119765786
1106	40-41	0.028182827770599772
1106	42-43	0.017998263237259948
1106	44-45	0.010814051617479947
1106	46-47	0.023424049010522197
1106	48-49	0.03276456569582109
1106	50-51	0.02556912852343629
1106	52-53	-0.010613373321113784
1106	54-55	0.028842830247782558
1106	56-57	-0.008621493284465487
1106	58-59	-0.016027857672149537
1106	60-61	0.03902164396636465
1106	62-63	-0.02632332557265471
1106	64-65	0.017052364220162985
1106	66-67	0.0094792661030354
1106	68-69	0.06591041014953447
1106	70-71	0.015434063436067902
1106	72-73	-0.015626708344186113
1106	74-75	-0.05645103204174262
1106	76-77	-0.021590447744799235
1106	78-79	0.04004226317218951
1106	80-81	0.07963494737614951
1106	82-83	0.03717743942677032
1106	84-85	0.10529259391517343
1106	86-87	0.10296939662077165
1106	88-89	0.13174167871574127
1106	90-91	0.1397768538956683
1106	92-93	0.077322726583823
1106	94-95	0.04944259071243806
1106	96-97	0.039906123712228236
1106	98-99	0.030077268362276754
1106	100	0.0692558879971159
1107	1	0.10663091935290936
1107	2	0.04247678095538987
1107	3	-0.0144493485743169
1107	4	0.003595299038359201
1107	5	0.011742310879419904
1107	6	0.0038561356136028735
1107	7	-2.159173052262986E-4
1107	8	-0.012349995063082986
1107	9	-0.02430411985588421
1107	10-11	-0.007529099565907416
1107	12-13	-0.03994982223441923
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2316	5	-0.09826452845384637
2316	6	-0.03872397416853346
2316	7	-0.04133634589210544
2316	8	0.0015088468792399112
2316	9	0.012029838113889468
2316	10-11	0.002651236309539229
2316	12-13	-0.013058070729634608
2316	14-15	-0.0021327495347520653
2316	16-17	0.006665871443352955
2316	18-19	-0.0035872078638519156
2316	20-21	-0.031731831476271566
2316	22-23	-0.011540362639948398
2316	24-25	-0.03828583409976005
2316	26-27	-0.025329742054026383
2316	28-29	-0.017335541524204245
2316	30-31	-0.3990095187961131
2316	32-33	-0.19218924830767747
2316	34-35	-0.27421428919178936
2316	36-37	-0.5506660186678118
2316	38-39	-0.3602339578009506
2316	40-41	-0.28810751652080313
2316	42-43	-0.41400716143458993
2316	44-45	-0.2546027187012996
2316	46-47	-0.39912526259845293
2316	48-49	-0.3756383044522025
2316	50-51	-0.42087440900031936
2316	52-53	-0.4477144361007319
2316	54-55	-0.28893891451475184
2316	56-57	-0.9618263917843635
2316	58-59	-0.7539745453738504
2316	60-61	-0.4559895694994367
2316	62-63	-0.2521010001919066
2316	64-65	-0.19632548628332103
2316	66-67	-0.19496918446012756
2316	68-69	-0.2716618360748555
2316	70-71	-0.2508169124449964
2316	72-73	-0.19114921368473858
2316	74-75	-0.17685952570443675
2316	76-77	-0.2166376697413881
2316	78-79	-0.25877185094497435
2316	80-81	-0.2499207249466764
2316	82-83	-0.42691658897079776
2316	84-85	-0.2840011111019223
2316	86-87	-0.3847064558790336
2316	88-89	-0.37461483969033793
2316	90-91	-0.2909853007594805
2316	92-93	-0.29303772221147284
2316	94-95	-0.2437766981551306
2316	96-97	-0.40296854657534453
2316	98-99	-0.21624720673499098
2316	100	-0.486785651396044
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	13.0
13	124.0
14	471.0
15	1597.0
16	3915.0
17	9193.0
18	17810.0
19	27427.0
20	42341.0
21	64984.0
22	85958.0
23	86005.0
24	76557.0
25	73184.0
26	80202.0
27	95726.0
28	118275.0
29	145134.0
30	178282.0
31	222862.0
32	283081.0
33	372886.0
34	576492.0
35	901860.0
36	1646305.0
37	3227446.0
38	3136838.0
39	833840.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76662215443703	21.278161105464307	13.911173765401172	23.044042974697494
2	15.563002413061305	20.106336532279308	40.49074949836889	23.83991155629049
3	18.390204608224543	26.683790132870026	29.952963953383982	24.973041305521445
4	12.666346081742983	17.185269244621164	39.38848060213421	30.759904071501648
5	13.153195518119984	38.46579512048642	34.64315226850267	13.737857092890929
6	29.7634187624229	38.26376509656979	17.791558025958732	14.181258115048582
7	26.629416168017368	32.68418881334655	23.045404965732974	17.640990052903106
8	26.469641285541133	34.68719430076081	19.816155356571695	19.02700905712636
9	26.334183637406817	15.197905713903634	21.662142165056483	36.80576848363307
10-11	25.646160756092666	26.08119911792384	27.130113707427245	21.142526418556244
12-13	25.83021120625082	23.9302469851935	27.732417704369745	22.50712410418594
14-15	23.42947314698601	24.966530258360653	25.512140463806652	26.091856130846686
16-17	22.021451693758625	27.806780477705118	26.737965416357085	23.433802412179176
18-19	21.547430670925845	27.447028332663216	29.18650782850672	21.819033167904223
20-21	23.08789638319163	26.56187710168203	28.5183774882725	21.831849026853842
22-23	23.415043308903044	26.151526444156143	28.29324892612265	22.14018132081816
24-25	22.853321355030115	26.442307457956105	28.8021607416149	21.90221044539887
26-27	22.64383568488335	26.660485108262645	28.125044049290683	22.570635157563324
28-29	21.924275905787578	26.93662645584429	28.647584502812055	22.491513135556072
30-31	22.787321823174235	26.441893070709167	28.532722416212096	22.238062689904503
32-33	23.048050317286283	26.847254716798695	27.74213463325723	22.362560332657793
34-35	22.897000499532155	26.78524244859808	28.51304844941904	21.80470860245073
36-37	22.751389924293054	26.427115559229968	28.171840753372486	22.649653763104492
38-39	22.835763655147673	26.394578688922195	28.608905000302663	22.16075265562747
40-41	22.52159129144406	25.75089007123576	29.164922763259536	22.56259587406064
42-43	22.52078375812173	25.973491377655634	28.49107968133365	23.014645182888987
44-45	22.57718106410634	26.730417245421574	28.224971810706055	22.46742987976603
46-47	22.64442895697993	26.56099667846426	27.66134781131415	23.133226553241663
48-49	23.37160330334487	26.403862051952963	27.67183673714803	22.552697907554137
50-51	23.01476392758342	26.367456678950475	27.396430241444524	23.22134915202158
52-53	22.404749381185603	26.89508404712495	27.311557956337122	23.388608615352315
54-55	22.484021462739697	26.83131291609346	27.222231813756686	23.462433807410157
56-57	23.26349888472943	26.44271157857246	26.921507545375817	23.372281991322293
58-59	22.33506202226179	26.833257689906198	27.639425838968744	23.192254448863263
60-61	22.52368402976243	27.244200921863325	27.033913620615756	23.198201427758484
62-63	22.87545554307803	26.607249814614303	26.80979600159967	23.707498640707996
64-65	22.11741973347996	26.77905880540761	27.110023440010895	23.993498021101534
66-67	22.75100087401296	26.414689657155865	27.072705724110556	23.76160374472062
68-69	22.248597415292462	26.417074453979883	27.321064833470686	24.013263297256966
70-71	22.282931701449964	26.154079382770277	27.36823060273644	24.19475831304332
72-73	22.79326825759735	26.308746462782896	27.368620827672142	23.52936445194761
74-75	22.762818970660415	26.719298668961777	27.116969727844147	23.40091263253366
76-77	22.499453220183764	26.697032309893675	26.756410323072938	24.04710414684962
78-79	22.333215842059232	26.627583519986302	26.943851289762932	24.09534934819153
80-81	22.07287075414656	26.988322830386842	27.27526277338313	23.663543642083468
82-83	22.747475962002284	26.16880593895595	28.077258747178618	23.006459351863153
84-85	22.92350277498369	26.48916975208066	26.947860789110422	23.639466683825233
86-87	21.811423210141022	27.143078711482225	27.380043976671438	23.665454101705315
88-89	22.464229731752035	26.068879326355386	27.33850713228041	24.12838380961217
90-91	22.318911083672965	26.10737776850535	27.474108567639444	24.09960258018224
92-93	22.474922517916664	26.335682412663875	27.289308366180148	23.900086703239314
94-95	21.499241540214047	26.1985358569775	27.864169768250342	24.43805283455811
96-97	21.80411929319711	26.219785005116314	27.488212572200155	24.487883129486423
98-99	21.32522483576795	26.682623465398443	27.359566048302124	24.63258565053148
100	21.917858968814595	26.192728001559235	26.758258913211247	25.131154116414923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	735.0
1	520.5
2	422.5
3	556.0
4	487.0
5	492.0
6	857.0
7	1712.5
8	2932.0
9	3889.0
10	3750.0
11	3532.0
12	4201.0
13	5262.5
14	6570.0
15	8216.0
16	9997.0
17	11689.0
18	13332.0
19	14760.5
20	16199.5
21	17503.0
22	18849.0
23	20818.5
24	23284.5
25	26666.0
26	32150.5
27	39054.0
28	49523.0
29	68928.0
30	96561.5
31	125012.0
32	164698.0
33	206041.0
34	239723.5
35	274882.5
36	311255.5
37	369556.0
38	394575.5
39	378141.5
40	357351.0
41	331984.0
42	330569.5
43	350993.5
44	380559.0
45	443707.0
46	500146.5
47	534708.0
48	564062.0
49	557702.5
50	534421.5
51	534703.0
52	534775.5
53	514110.5
54	495644.5
55	443023.0
56	390202.0
57	328188.0
58	262409.0
59	239696.5
60	196882.5
61	142043.5
62	106782.0
63	86576.0
64	63122.5
65	34915.0
66	21820.5
67	16014.0
68	12271.5
69	9088.5
70	6703.5
71	4995.5
72	3696.0
73	2622.5
74	1962.5
75	1339.0
76	857.0
77	522.5
78	314.5
79	191.0
80	99.0
81	56.5
82	37.0
83	25.0
84	15.5
85	6.5
86	8.0
87	8.0
88	5.5
89	4.5
90	4.0
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35241916392028483
2	6.174383101948326E-4
3	0.002250400156894324
4	0.0013892361979383734
5	0.00491513391668255
6	0.0
7	3.2496753168149086E-5
8	4.1433360289390084E-4
9	0.0031196883041423123
10-11	6.093141219027954E-5
12-13	4.874512975222363E-5
14-15	3.493400965576027E-4
16-17	8.530397706639136E-5
18-19	0.001015523536504659
20-21	2.518498370531554E-4
22-23	1.706079541327827E-4
24-25	0.0
26-27	0.0021732203681199703
28-29	0.011154510524967175
30-31	0.0035177735304521385
32-33	0.014136087628144854
34-35	0.011422608738604404
36-37	0.012974328702383523
38-39	0.012730603053622403
40-41	0.014306695582277635
42-43	0.0066090271755723205
44-45	0.0013892361979383734
46-47	0.006174383101948327
48-49	0.012824031218980833
50-51	0.0035949533192264926
52-53	0.005751925310762389
54-55	0.004992313705456904
56-57	2.0716680144695042E-4
58-59	6.13376216048814E-4
60-61	3.858989438717704E-4
62-63	0.0010114614423586403
64-65	4.3870616777001264E-4
66-67	0.001462353892566709
68-69	0.0017710730476641254
70-71	9.545921243143794E-4
72-73	0.002766286113438691
74-75	0.008213554363249682
76-77	0.004013349016266412
78-79	0.0016776448823056967
80-81	6.052520277567767E-4
82-83	1.9904261315491315E-4
84-85	0.0016329618466994914
86-87	0.004695780832797544
88-89	0.0019173084369207961
90-91	1.9498051900889452E-4
92-93	0.0021935308388500636
94-95	0.0034527800241158403
96-97	0.002087916391053579
98-99	0.0022016550271421005
100	0.0025266225588235916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2308922E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.19412139629182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.71634891984215	31.67141697563944
2	16.606227936964387	16.00645130257109
3	6.975718608348881	10.085658883114135
4	3.6210826319782363	6.980595838062522
5	2.1356406575330804	5.146266255400288
6	1.3320971718076846	3.8519551685853943
7	0.8519606285754368	2.874164577089798
8	0.602286994040525	2.3221354004957404
9	0.4266591192066277	1.8506215247291242
>10	1.652530552637542	12.713950505740154
>50	0.05045595638305005	1.6618400213859443
>100	0.02614858097870228	2.383730559643029
>500	0.0018090491135778343	0.6264629169757789
>1k	9.157844004543456E-4	0.7338927477525675
>5k	4.696327585741209E-5	0.151350469798938
>10k+	7.044491378611814E-5	0.9395068530161532
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35049	0.28474467544761434	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30768	0.2499650253694028	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17202	0.13975228699962514	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.124188292037272E-6
7	0.0	0.0	0.0	8.124188292037272E-6	8.124188292037272E-6
8	0.0	0.0	0.0	8.124188292037272E-6	8.124188292037272E-6
9	0.0	0.0	0.0	1.6248376584074543E-5	2.4372564876111815E-5
10-11	0.0	0.0	0.0	2.0310470730093177E-5	2.4372564876111815E-5
12-13	0.0	0.0	0.0	2.4372564876111815E-5	2.4372564876111815E-5
14-15	0.0	4.062094146018636E-6	0.0	2.4372564876111815E-5	3.655884731416772E-5
16-17	0.0	8.124188292037272E-6	0.0	2.8434659022130452E-5	4.062094146018636E-5
18-19	0.0	1.2186282438055907E-5	0.0	4.874512975222363E-5	4.062094146018636E-5
20-21	0.0	1.6248376584074543E-5	0.0	6.90556004823168E-5	4.8745129752223636E-5
22-23	0.0	1.6248376584074543E-5	0.0	2.0716680144695042E-4	7.717978877435407E-5
24-25	0.0	1.6248376584074543E-5	0.0	6.296245926328886E-4	1.0561444779648454E-4
26-27	0.0	1.6248376584074543E-5	0.0	0.001551719963779119	1.0967654194250317E-4
28-29	0.0	1.6248376584074543E-5	0.0	0.00458610429085504	1.1373863608852181E-4
30-31	0.0	2.0310470730093177E-5	0.0	0.015911222769954998	1.2998701267259634E-4
32-33	0.0	2.4372564876111815E-5	0.0	0.041559285207916664	1.4623538925667088E-4
34-35	0.0	2.4372564876111815E-5	0.0	0.08277329241342174	1.4623538925667088E-4
36-37	0.0	2.8434659022130452E-5	0.0	0.15004157147149036	1.4623538925667088E-4
38-39	0.0	3.2496753168149086E-5	0.0	0.24522456150099903	1.4623538925667088E-4
40-41	0.0	3.655884731416772E-5	0.0	0.34560703203741155	1.4623538925667088E-4
42-43	0.0	4.062094146018636E-5	0.0	0.44951540029256826	1.5029748340268954E-4
44-45	0.0	4.062094146018636E-5	0.0	0.5745425960128758	1.584216716947268E-4
46-47	0.0	4.062094146018636E-5	0.0	0.7224312575869763	1.7467004827880135E-4
48-49	0.0	4.062094146018636E-5	0.0	0.8855568343027926	1.8685633071685725E-4
50-51	0.0	4.4683035606204995E-5	0.0	1.0546333789425264	1.8685633071685725E-4
52-53	0.0	4.874512975222363E-5	0.0	1.2188354106070378	2.031047073009318E-4
54-55	0.0	4.874512975222363E-5	0.0	1.3986358837922606	2.3153936632306225E-4
56-57	0.0	4.874512975222363E-5	0.0	1.6072162940020256	2.4372564876111815E-4
58-59	0.0	4.874512975222363E-5	0.0	1.8483828234511521	2.477877429071368E-4
60-61	0.0	4.874512975222363E-5	0.0	2.096491471795824	2.762224019292672E-4
62-63	0.0	4.874512975222363E-5	0.0	2.3478904163987715	2.843465902213045E-4
64-65	0.0	4.874512975222363E-5	0.0	2.606219293614827	2.9247077851334176E-4
66-67	0.0	6.499350633629817E-5	0.0	2.8915123517721537	3.005949668053791E-4
68-69	0.0	6.499350633629817E-5	0.0	3.2052035101042966	3.005949668053791E-4
70-71	0.0	6.499350633629817E-5	0.0	3.549230387518907	3.0465706095139774E-4
72-73	0.0	6.499350633629817E-5	0.0	3.90078432538609	3.412159082655654E-4
74-75	0.0	6.499350633629817E-5	0.0	4.243072626506205	3.412159082655654E-4
76-77	0.0	6.499350633629817E-5	0.0	4.62701770309374	3.412159082655654E-4
78-79	0.0	6.499350633629817E-5	0.0	5.040782612807198	3.412159082655654E-4
80-81	0.0	6.499350633629817E-5	0.0	5.486154677070827	3.4527800241158403E-4
82-83	0.0	6.499350633629817E-5	0.0	5.9421531796204405	3.5746428484963996E-4
84-85	0.0	7.311769462833544E-5	0.0	6.3856282459178795	3.5746428484963996E-4
86-87	0.0	7.311769462833544E-5	0.0	6.853362138455342	3.777747555797331E-4
88	0.0	7.311769462833544E-5	0.0	7.220811050715895	3.8996103801778903E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24395	0.0	50.299206	1
GTATCAA	43185	0.0	39.628597	1
CAACGCA	49655	0.0	33.201378	5
GTGGTAT	6975	0.0	32.520374	1
ATCAACG	51000	0.0	32.308525	3
TATCAAC	54750	0.0	31.159147	2
AACGCAG	56325	0.0	30.069693	6
TCAACGC	59715	0.0	27.883724	4
ACGCAGA	59510	0.0	27.583796	7
CGCAGAG	60260	0.0	27.1625	8
TAGGCAT	13690	0.0	26.398693	5
GGGCACC	11365	0.0	25.514221	94
GCAGAGT	64550	0.0	25.16081	9
AGGGCTA	13040	0.0	24.579119	5
TATTCTC	7070	0.0	24.062939	5
CTAGGCA	15430	0.0	23.87884	4
GCTATTG	13185	0.0	23.809032	8
GTACATG	57730	0.0	23.640226	1
GAGTACT	32895	0.0	23.329205	12-13
GGGCTAT	14210	0.0	23.282198	6
>>END_MODULE