FastQCFastQC Report
Thu 26 May 2016
ERR580105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7608
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC100.13144058885383808No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT90.11829652996845426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10515247108307045No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA200.002081008670.499994
AACGCAG250.005038714556.3999985
Produced by FastQC (version 0.11.5)