FastQCFastQC Report
Thu 26 May 2016
ERR580105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7608
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT370.48633017875920087No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG240.31545741324921134TruSeq Adapter, Index 3 (96% over 28bp)
GTATAAAATCAGGCAGTTTTTGATCACGTATCTCGTATGCCGTCTTCTGC240.31545741324921134TruSeq Adapter, Index 2 (96% over 25bp)
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA220.2891692954784437No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA210.27602523659305994No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG190.24973711882229233RNA PCR Primer, Index 17 (96% over 28bp)
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG170.2234490010515247No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG160.2103049421661409RNA PCR Primer, Index 26 (100% over 29bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC150.1971608832807571No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG150.1971608832807571RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG150.1971608832807571TruSeq Adapter, Index 10 (96% over 28bp)
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG130.17087276550998948No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA130.17087276550998948No Hit
CAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTA120.15772870662460567No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG110.14458464773922186RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.14458464773922186No Hit
GTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAAT100.13144058885383808No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG100.13144058885383808RNA PCR Primer, Index 33 (96% over 29bp)
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT100.13144058885383808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.13144058885383808No Hit
GATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCA90.11829652996845426No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG90.11829652996845426No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC90.11829652996845426No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG90.11829652996845426No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA90.11829652996845426No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC90.11829652996845426No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC80.10515247108307045No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT80.10515247108307045No Hit
GGTTGATCCCTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCC80.10515247108307045No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC80.10515247108307045No Hit
GTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGC80.10515247108307045No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG200.002081008670.499992
CCCACGA853.311343E-738.705889
TCTCCGA853.311343E-738.705881
TCCGAGC853.311343E-738.705883
CTCCGAG853.311343E-738.705882
GAGCCCA905.1844836E-736.5555576
AGCCCAC957.918952E-734.631587
GCCCACG957.918952E-734.631588
CGAGCCC1001.1830198E-632.8999985
CCGAGCC1051.7322855E-631.3333324
TATGCCG905.084152E-620.8888932-33
CCGTCTT905.084152E-620.8888936-37
CACGAGA905.084152E-620.8888910-11
TCTGCTT905.084152E-620.8888942-43
ACGAGAC804.976896E-520.56249812-13
TGCCGTC958.118906E-619.78947434-35
TGCTTGA757.788353E-418.80000144-45
CTTGAAA757.788353E-418.80000146-47
TGAAAAA757.788353E-418.80000148-49
ATGCCGT901.2309231E-418.27777934-35
Produced by FastQC (version 0.11.5)