FastQCFastQC Report
Thu 26 May 2016
ERR580104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737468
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119150.040.6537861
GGTATCA67350.040.569191
CAACGCA132550.034.7272035
ATCAACG138700.033.3621253
TATCAAC149000.032.2256052
AACGCAG151500.031.2972956
TCAACGC151250.030.87214
ACGCAGA169500.026.8642547
CGCAGAG176950.025.600658
GTACATG251050.025.2327841
GTACTAG29700.025.1766821
TACATGG254900.024.519792
ACATGGG260500.023.2510833
GTGGTAT32600.021.927231
GCAGAGT200800.021.815059
CGTTAAC8650.021.7471181
CATGGGG211850.021.2642174
TAGTACT40000.020.6902184
TAGGCAT43200.020.5705035
ATGGGGG146400.019.6873055
Produced by FastQC (version 0.11.5)