FastQCFastQC Report
Thu 26 May 2016
ERR580104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737468
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75900.041.9463651
GTATCAA124000.037.5048641
CAACGCA138000.032.2170645
ATCAACG142950.031.1683083
TATCAAC152400.030.1908572
AACGCAG157450.028.9819586
TCAACGC158250.028.4513764
GTACTAG30350.026.1085621
ACGCAGA174000.025.3340577
GTACATG250350.024.737141
CGCAGAG182500.024.0253628
TACATGG253750.023.8924872
ACATGGG259100.022.7286013
GCAGAGT207700.020.635069
CATGGGG209550.020.4096344
GTGGTAT31250.019.4702721
ATGGGGG142800.018.8582045
TAGGCAT43550.018.7773785
TACCCCG7600.018.5516075
TAGTACT45850.018.2457684
Produced by FastQC (version 0.11.5)