FastQCFastQC Report
Thu 26 May 2016
ERR580103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5861633
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158960.27118722717713645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128540.21929042640506494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72480.12365154897961028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91950.052.7333221
GTATCAA165850.041.288031
CAACGCA195700.034.0863535
ATCAACG196650.034.040343
TATCAAC207150.032.7295342
AACGCAG211800.031.8549146
TCAACGC212350.031.5918874
GTACTAG29150.028.87971
ACGCAGA234000.028.2505047
GTACATG242050.027.4546131
CGCAGAG240200.027.423748
TACATGG246350.026.4717942
ACATGGG247950.025.3860033
GTGGTAT35800.024.6975041
GCAGAGT264900.024.5313559
CATGGGG195300.023.2048444
GCATCGT31900.022.3874478
GAGTACT138700.022.27564412-13
ATGGGGG145850.022.014225
TAGTACT39500.021.7799994
Produced by FastQC (version 0.11.5)