FastQCFastQC Report
Thu 26 May 2016
ERR580103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5861633
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141650.24165620740841331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121620.2074848425344951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70890.120938994304147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.049.983841
GTATCAA164100.041.7470361
CAACGCA198850.033.3749585
ATCAACG200650.033.1232533
TATCAAC211700.032.0713582
AACGCAG215200.031.359976
TCAACGC213800.031.3272674
GTACATG244400.027.742061
ACGCAGA238900.027.6390847
GTACTAG30900.027.3897911
TACATGG245750.027.1685092
CGCAGAG245400.026.7724828
ACATGGG246200.026.4986743
GTGGTAT37550.026.0452231
CATGGGG197300.025.513954
ATGGGGG143700.024.2366735
GCAGAGT272100.023.816859
CTGTGCG39750.022.5810899
TGGTATC41700.022.4381982
CTAACGC14700.022.3822363
Produced by FastQC (version 0.11.5)