##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580103_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5861633
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49006991737627	34.0	31.0	34.0	31.0	34.0
2	32.613219558440456	34.0	31.0	34.0	31.0	34.0
3	32.66641275562629	34.0	31.0	34.0	31.0	34.0
4	36.194963587792	37.0	35.0	37.0	35.0	37.0
5	36.13651946479761	37.0	35.0	37.0	35.0	37.0
6	36.173109268355766	37.0	36.0	37.0	35.0	37.0
7	36.12072608435226	37.0	36.0	37.0	35.0	37.0
8	36.11398803029804	37.0	36.0	37.0	35.0	37.0
9	37.82689107284608	39.0	38.0	39.0	35.0	39.0
10-11	37.7753664379875	39.0	38.0	39.0	35.0	39.0
12-13	37.73549717288681	39.0	38.0	39.0	35.0	39.0
14-15	39.1065825854331	40.0	38.0	41.0	36.0	41.0
16-17	39.090691962461655	40.0	38.0	41.0	36.0	41.0
18-19	39.08525311973643	40.0	38.5	41.0	35.5	41.0
20-21	39.05869183553457	40.0	39.0	41.0	35.0	41.0
22-23	38.98462237400397	40.0	38.0	41.0	35.0	41.0
24-25	38.91230933427596	40.0	38.0	41.0	35.0	41.0
26-27	38.79066857307511	40.0	38.0	41.0	35.0	41.0
28-29	38.68576435952234	40.0	38.0	41.0	34.5	41.0
30-31	38.53528137977932	40.0	38.0	41.0	34.0	41.0
32-33	38.429384610056616	40.0	38.0	41.0	34.0	41.0
34-35	38.340105308537744	40.0	38.0	41.0	34.0	41.0
36-37	38.22276752911688	40.0	38.0	41.0	34.0	41.0
38-39	38.113299740874254	40.0	38.0	41.0	33.0	41.0
40-41	38.00899971049023	40.0	37.0	41.0	33.0	41.0
42-43	38.019288225653156	40.0	37.0	41.0	33.0	41.0
44-45	37.998583500536455	40.0	37.0	41.0	33.0	41.0
46-47	37.82793873311414	40.0	37.0	41.0	33.0	41.0
48-49	37.64335484667839	40.0	37.0	41.0	33.0	41.0
50-51	37.46433024721951	40.0	36.5	41.0	32.5	41.0
52-53	37.306568237895476	40.0	36.0	41.0	32.0	41.0
54-55	37.09562403514515	39.0	35.5	41.0	32.0	41.0
56-57	36.87133645180447	39.0	35.0	41.0	31.0	41.0
58-59	36.63608639435461	39.0	35.0	41.0	31.0	41.0
60-61	36.37695246358822	38.0	35.0	40.0	31.0	41.0
62-63	36.07246879837069	38.0	35.0	40.0	30.5	41.0
64-65	35.769868140840615	37.0	35.0	40.0	30.0	41.0
66-67	35.44757535997221	36.5	34.5	39.5	29.5	41.0
68-69	35.12006244676185	36.0	34.0	39.0	29.0	41.0
70-71	34.77897456220818	35.5	34.0	39.0	29.0	40.5
72-73	34.4496786987517	35.0	34.0	38.0	29.0	40.0
74-75	34.089645069897756	35.0	34.0	37.0	29.0	39.5
76-77	32.55424623138296	34.0	31.5	36.0	26.5	38.5
78-79	33.44323527249148	35.0	33.0	36.0	28.0	39.0
80-81	33.420796303692164	35.0	33.0	36.0	29.0	38.0
82-83	33.18978167005679	35.0	33.0	36.0	28.0	37.0
84-85	32.97561012434589	35.0	33.0	35.0	27.5	37.0
86-87	32.77501568248985	35.0	33.0	35.0	27.0	36.0
88-89	32.59916639271002	35.0	33.0	35.0	27.0	36.0
90-91	32.43437801377193	35.0	33.0	35.0	27.0	36.0
92-93	32.27555708451894	35.0	33.0	35.0	26.0	35.5
94-95	32.14388985117287	35.0	32.5	35.0	25.5	35.0
96-97	32.01962959810005	35.0	32.0	35.0	25.0	35.0
98-99	31.915951152178923	34.5	32.0	35.0	25.0	35.0
100	30.012345194589972	33.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08724168095231732
1101	2	-0.047596494982173
1101	3	-0.052497421237788444
1101	4	-0.06594517964963131
1101	5	-0.06374025941406103
1101	6	-0.07244471847042178
1101	7	-0.039044912867716164
1101	8	-0.021834444633945793
1101	9	-0.0615264059506444
1101	10-11	-0.11962326462298023
1101	12-13	-0.04116143029814623
1101	14-15	-0.0796927613914562
1101	16-17	-0.05380547761824772
1101	18-19	-0.08165718490825213
1101	20-21	-0.05559944886083912
1101	22-23	-0.16721659578254133
1101	24-25	-0.16214732082886485
1101	26-27	-0.12702890497755703
1101	28-29	-0.11107990988218575
1101	30-31	-0.14263493585782783
1101	32-33	-0.14582800593985468
1101	34-35	-0.13358052878179194
1101	36-37	-0.15591385478337827
1101	38-39	-0.18086534782315766
1101	40-41	-0.09977093337629128
1101	42-43	-0.09767308512868311
1101	44-45	-0.07204908517163489
1101	46-47	-0.07813257061474133
1101	48-49	-0.08396051228056223
1101	50-51	-0.05350003842777795
1101	52-53	-0.0888133779307907
1101	54-55	-0.06333126400051725
1101	56-57	-0.07181307353923927
1101	58-59	-0.048944109373977085
1101	60-61	-0.0966348686836298
1101	62-63	-0.1347172496531286
1101	64-65	-0.12954120629521526
1101	66-67	-0.1855380737460095
1101	68-69	-0.17470357978425
1101	70-71	-0.12141396258942194
1101	72-73	-0.15451159910536916
1101	74-75	-0.11118251684943914
1101	76-77	-0.14112077858861483
1101	78-79	-0.22800009798599774
1101	80-81	-0.09002590754767681
1101	82-83	-0.042117670735962065
1101	84-85	-0.09346314926021648
1101	86-87	-0.19562969897379645
1101	88-89	-0.11355976070502294
1101	90-91	-0.09891022888996304
1101	92-93	-0.14009395217807707
1101	94-95	-0.1750944933323737
1101	96-97	-0.2760769296653862
1101	98-99	-0.23265693797890563
1101	100	-0.42866090230292286
1102	1	-0.05084451302749926
1102	2	-0.10458616274616617
1102	3	-0.07363274659572028
1102	4	-0.0392723674166362
1102	5	-0.02379608013927026
1102	6	-0.017587772677700286
1102	7	-0.03829516341829731
1102	8	-0.052824675472507465
1102	9	-0.06927830274286606
1102	10-11	-0.023318898983959002
1102	12-13	-0.061632341497315224
1102	14-15	-0.029304300307458675
1102	16-17	-0.01591843223854994
1102	18-19	-0.09815003777895726
1102	20-21	-0.09878786270542861
1102	22-23	-0.10745294938356409
1102	24-25	-0.09568038926016698
1102	26-27	-0.03887874454783713
1102	28-29	-0.06599045906603607
1102	30-31	-0.0930289088343983
1102	32-33	-0.09787606549269157
1102	34-35	-0.21816261130121006
1102	36-37	-0.21671575245913033
1102	38-39	-0.31925180274175347
1102	40-41	-0.23231420047530804
1102	42-43	-0.14729117039970419
1102	44-45	-0.1987295442515986
1102	46-47	-0.1345793028107778
1102	48-49	-0.13704903038262017
1102	50-51	-0.2569896952811703
1102	52-53	-0.29719681247060237
1102	54-55	-0.2621454493292745
1102	56-57	-0.1976754583405338
1102	58-59	-0.20959515161218434
1102	60-61	-0.3111028289293287
1102	62-63	-0.3512323135958013
1102	64-65	-0.33867476759597537
1102	66-67	-0.22131564897736666
1102	68-69	-0.26181888474094706
1102	70-71	-0.2315655041051059
1102	72-73	-0.3126546987786867
1102	74-75	-0.28953915409565667
1102	76-77	-0.3658010369388478
1102	78-79	-0.29634839916285216
1102	80-81	-0.3120890859734615
1102	82-83	-0.26223572962662445
1102	84-85	-0.2876539163339231
1102	86-87	-0.3822702265458702
1102	88-89	-0.28455143413132333
1102	90-91	-0.31653977675944844
1102	92-93	-0.3420444924221542
1102	94-95	-0.37793284760261514
1102	96-97	-0.3738557029866243
1102	98-99	-0.4172867566951801
1102	100	-0.3075613523664025
1103	1	-0.013695265803498557
1103	2	0.0076581681037311
1103	3	0.008035027366602776
1103	4	-0.001583263162480364
1103	5	0.008591030753201778
1103	6	0.012820193251293688
1103	7	0.004161194442623639
1103	8	-0.003510269399910726
1103	9	-0.05080162067743288
1103	10-11	0.02610774599207133
1103	12-13	0.006799739637735058
1103	14-15	7.682049696882132E-4
1103	16-17	-0.029288290718284316
1103	18-19	0.02634322016440649
1103	20-21	-0.004995635492946349
1103	22-23	-0.0450157561835951
1103	24-25	-0.03909338916135141
1103	26-27	-0.014991086665169462
1103	28-29	-0.04421025019312452
1103	30-31	-0.05532042096193379
1103	32-33	-0.023372864528489856
1103	34-35	-0.03649741768615655
1103	36-37	-0.017545507783282233
1103	38-39	-0.04626250578652247
1103	40-41	-0.020954280550434135
1103	42-43	0.060381799240708744
1103	44-45	0.026103587605817324
1103	46-47	-0.01397562374420147
1103	48-49	0.03636466546800676
1103	50-51	9.048957961184101E-4
1103	52-53	-0.006104704679820827
1103	54-55	0.04145520198510866
1103	56-57	0.03387240700826766
1103	58-59	0.012258020301409545
1103	60-61	-0.05625554423930623
1103	62-63	0.01531076105295881
1103	64-65	-0.04648988893960393
1103	66-67	8.691999057717226E-4
1103	68-69	-0.016623789087908847
1103	70-71	-0.03951255190560232
1103	72-73	-0.07007713592784626
1103	74-75	-0.04221193796347933
1103	76-77	-0.05580346593882979
1103	78-79	-0.08767123403904975
1103	80-81	-0.06348329536405117
1103	82-83	-0.09096082221984148
1103	84-85	-0.05997195083378415
1103	86-87	-0.06269358151711657
1103	88-89	-0.13981829817388558
1103	90-91	-0.14972677669698697
1103	92-93	-0.11963132002226473
1103	94-95	-0.1282036600106906
1103	96-97	-0.06910109060037328
1103	98-99	-0.0998512261444553
1103	100	0.07741693891150803
1104	1	-0.026002372227175385
1104	2	-0.057029123243900415
1104	3	-0.03205734126913029
1104	4	-0.026976755998269653
1104	5	-0.053028714214612194
1104	6	-0.06644006946375924
1104	7	0.02133791925556494
1104	8	-0.0021790426323349266
1104	9	0.024749957929763866
1104	10-11	-0.02543829613882309
1104	12-13	-0.031027593737491088
1104	14-15	-0.09736984063260223
1104	16-17	-0.08944136312868523
1104	18-19	-0.09252860974554977
1104	20-21	-0.07082104483273355
1104	22-23	-0.10080604295738738
1104	24-25	-0.07863298133469954
1104	26-27	-0.10330734200599778
1104	28-29	-0.09880651906608762
1104	30-31	-0.09411112029572166
1104	32-33	-0.16386395818997812
1104	34-35	-0.061604980933431364
1104	36-37	-0.05944513394875628
1104	38-39	-0.10523203504706657
1104	40-41	-0.11694178718011727
1104	42-43	-0.15564863520010164
1104	44-45	-0.17026117599564117
1104	46-47	-0.15909987606995202
1104	48-49	-0.06518420795697466
1104	50-51	-0.051499349941948935
1104	52-53	-0.09084077310546235
1104	54-55	-0.04323647838688771
1104	56-57	-0.12594781722607706
1104	58-59	-0.0903128154613384
1104	60-61	-0.2542186926134278
1104	62-63	-0.18040996092910433
1104	64-65	-0.17404186496507634
1104	66-67	-0.15200522732758515
1104	68-69	-0.17249586188763288
1104	70-71	-0.14672370235061294
1104	72-73	-0.1423211822764472
1104	74-75	-0.09127473814027809
1104	76-77	-0.15528238337642364
1104	78-79	-0.1100591993176323
1104	80-81	-0.009673877339146486
1104	82-83	-0.004431393458830257
1104	84-85	-0.05438154512489746
1104	86-87	-0.05516669511298744
1104	88-89	-0.055870428831575225
1104	90-91	-0.020205962329697513
1104	92-93	-0.02043864330440215
1104	94-95	-0.06572394835880147
1104	96-97	-0.035047260336554587
1104	98-99	-0.09137248563681055
1104	100	0.028978579087425516
1105	1	2.5067407015910703E-4
1105	2	0.012788832641305703
1105	3	0.0119045823111108
1105	4	0.03756228835517561
1105	5	-0.0216977939648757
1105	6	-0.016074562669757597
1105	7	0.015922787581708064
1105	8	-0.004063291859928597
1105	9	0.0021485143988471123
1105	10-11	-0.027391316839889157
1105	12-13	-0.005210339744039061
1105	14-15	-0.002664433153064749
1105	16-17	-0.028097280683205383
1105	18-19	-0.0533383020401601
1105	20-21	-0.03613783516870939
1105	22-23	-0.07203329786731416
1105	24-25	-0.06393029308181752
1105	26-27	-0.06665401582615971
1105	28-29	-0.03005211265218577
1105	30-31	-0.054991553892740797
1105	32-33	-0.10707955527834656
1105	34-35	-0.10517434779848145
1105	36-37	-0.09935652845450704
1105	38-39	-0.010032502445774583
1105	40-41	-0.042850697170813135
1105	42-43	-0.03535707636000751
1105	44-45	0.0070875136065566835
1105	46-47	-0.04161113939372285
1105	48-49	-0.1412662199017305
1105	50-51	-0.1179708396298409
1105	52-53	-0.17056672417553642
1105	54-55	-0.15915552019997392
1105	56-57	-0.13461325293656756
1105	58-59	-0.1315763178120406
1105	60-61	-0.13433888553704065
1105	62-63	-0.11667853206665058
1105	64-65	-0.09246644580775865
1105	66-67	-0.16645213976160989
1105	68-69	-0.13183721205095367
1105	70-71	-0.06195547803235968
1105	72-73	-0.12493297014121652
1105	74-75	-0.10316002461940599
1105	76-77	-0.1286868887864543
1105	78-79	-0.08805293169575634
1105	80-81	-0.07206480165563534
1105	82-83	0.011700714830695347
1105	84-85	0.010845990392198246
1105	86-87	-0.004901592082156014
1105	88-89	-0.008725448586943685
1105	90-91	-0.032116942855047625
1105	92-93	0.003493398114457591
1105	94-95	0.08141693981779952
1105	96-97	0.08778609676412685
1105	98-99	0.03634104360564194
1105	100	-0.06666837787336277
1106	1	0.013416976078531206
1106	2	-0.012260686188000136
1106	3	0.009968191203576282
1106	4	0.02506020192016223
1106	5	0.021822607028830987
1106	6	0.007522785815702093
1106	7	-0.027087232630961466
1106	8	0.020014775882479796
1106	9	-0.01660494804150403
1106	10-11	-0.017728937908096043
1106	12-13	-0.01506750084043773
1106	14-15	-0.001964363308132988
1106	16-17	0.03658236386003466
1106	18-19	0.024074687542835704
1106	20-21	0.038060727812919026
1106	22-23	0.06361112348583475
1106	24-25	0.07940310430112163
1106	26-27	0.02551743403376605
1106	28-29	0.05247203501162545
1106	30-31	0.013380979339160604
1106	32-33	0.03767995371433841
1106	34-35	-0.0169449434296709
1106	36-37	-0.035481550418509755
1106	38-39	-0.02046904715660247
1106	40-41	0.019982568138757983
1106	42-43	0.017323989737100476
1106	44-45	-0.03705889023604669
1106	46-47	-0.023561812597463927
1106	48-49	0.03679400728760385
1106	50-51	-0.016252719720192488
1106	52-53	0.004102395012047566
1106	54-55	-0.01172223461059474
1106	56-57	-0.01536673493905738
1106	58-59	0.01934707292439697
1106	60-61	-0.06895238517090974
1106	62-63	-0.04574155027518856
1106	64-65	-0.0071168571103683576
1106	66-67	-0.0474801427869167
1106	68-69	-0.009355243942565039
1106	70-71	-0.04673972386800074
1106	72-73	-0.06380517012228637
1106	74-75	0.03863914851569206
1106	76-77	-0.0757417917177392
1106	78-79	0.006483872786354539
1106	80-81	0.033198318924213766
1106	82-83	-1.262897848164357E-4
1106	84-85	0.02698947021622189
1106	86-87	0.013543940330912108
1106	88-89	-0.01008174709728138
1106	90-91	0.021199603309632664
1106	92-93	0.041361455668351255
1106	94-95	0.07347809800279492
1106	96-97	-0.006937011027410733
1106	98-99	0.08693553000000875
1106	100	0.03641893245782768
1107	1	0.027221879463546372
1107	2	0.05249202389672547
1107	3	0.02376750941554917
1107	4	0.018587269880093515
1107	5	-0.022608408429341864
1107	6	-0.011154287984560085
1107	7	-2.749452738015634E-5
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2316	42-43	-0.23710127110153678
2316	44-45	-0.2582164280026831
2316	46-47	-0.21573432593292807
2316	48-49	-0.18238415406100472
2316	50-51	-0.1749513034630965
2316	52-53	-0.25940710269589573
2316	54-55	-0.25206956658416146
2316	56-57	-0.3557604192953363
2316	58-59	-0.3390532614768702
2316	60-61	-0.22571101074002087
2316	62-63	-0.2033515068299323
2316	64-65	-0.15961931607090207
2316	66-67	-0.24113728338694074
2316	68-69	-0.16363784491945665
2316	70-71	-0.1120448758786381
2316	72-73	-0.14252923655284633
2316	74-75	-0.2152924483241705
2316	76-77	-0.11595870980028167
2316	78-79	-0.16146172102982348
2316	80-81	-0.1380177954927646
2316	82-83	-0.13971432274226459
2316	84-85	-0.11999478722761125
2316	86-87	-0.18377028010046814
2316	88-89	-0.19954818304559296
2316	90-91	-0.23874334359722127
2316	92-93	-0.17805348643430818
2316	94-95	-0.1143137272230561
2316	96-97	-0.19367227538628384
2316	98-99	-0.1099672540294705
2316	100	-0.2352535600426684
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	38.0
14	228.0
15	671.0
16	1859.0
17	4276.0
18	7500.0
19	11913.0
20	17740.0
21	26165.0
22	34352.0
23	36042.0
24	35284.0
25	36407.0
26	41809.0
27	50646.0
28	62505.0
29	77213.0
30	96561.0
31	121400.0
32	154132.0
33	204214.0
34	306461.0
35	450294.0
36	776354.0
37	1441805.0
38	1532906.0
39	332740.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.434042666447375	19.982283875306734	13.604939632270984	23.978733825974903
2	15.589491369519715	20.67641053252226	40.04621353623146	23.68788456172656
3	18.37201589475559	26.62502925844264	29.821245416745995	25.181709430055772
4	12.790047001219815	17.231739045133114	37.9988910782984	31.97932287534867
5	13.295762094621583	38.177761937316475	34.48912777730101	14.037348190760934
6	30.71293638238231	37.12466717881081	18.15465695469401	14.007739484112868
7	27.03633225785319	32.56604717372553	22.695548358391473	17.702072210029808
8	26.106172042467723	35.291998026344075	20.096314682102857	18.50551524908534
9	26.840011696532972	15.539755075691378	20.488569095699	37.13166413207665
10-11	25.365481456270388	25.94870784386733	28.050636193849222	20.63517450601306
12-13	25.817117205568387	23.67455562903722	28.05864445349957	22.449682711894823
14-15	23.32096522747613	25.0161986985766	25.76669574869037	25.8961403252569
16-17	22.532383494824305	27.638857327691213	26.63309578840554	23.195663389078938
18-19	22.229608371269688	27.27990887086263	28.555027559159896	21.935455198707785
20-21	23.28184613174672	26.580832580625984	28.134626980585654	22.002694307041647
22-23	23.33455343601929	26.216770932701255	27.900589411106676	22.548086220172774
24-25	22.892650210588048	26.22462014181372	28.246248333955325	22.636481313642907
26-27	22.728923325979427	26.47653305006159	27.969987935701546	22.824555688257437
28-29	22.411462631241427	26.914193189904296	28.087836112229027	22.586508066625257
30-31	23.32336293459842	26.415130552796267	28.037415225664684	22.224091286940624
32-33	23.181324128457224	26.552943249809648	27.71675181791242	22.548980803820708
34-35	23.135332683282016	26.73061235874883	27.983088543318928	22.150966414650227
36-37	23.005201572559322	26.545157595015045	27.82875014256405	22.62089068986158
38-39	23.011372966193132	26.62442887582016	27.993207628222137	22.370990529764565
40-41	22.996383889640178	26.06768492590134	28.293259205530546	22.64267197892793
42-43	22.743608431714367	26.337267209813913	27.83322533854575	23.085899019925975
44-45	22.618124004026676	26.731721498283388	27.913902011195418	22.736252486494514
46-47	22.617589755488375	26.430210458791976	27.5054167206917	23.446783065027947
48-49	22.92539175956821	26.482154296083397	27.622172912079385	22.970281032269003
50-51	22.88060422647335	26.53671250106206	27.48410491557597	23.098578356888623
52-53	22.68457601014097	26.763714471311967	27.272852902626475	23.27885661592059
54-55	22.4353153975764	26.599286445953535	27.52975246034882	23.43564569612124
56-57	22.919454496769422	26.498710592589998	27.089284862772434	23.492550047868146
58-59	22.499960759612016	26.733077326767337	27.528164361947876	23.238797551672764
60-61	22.842789855239367	26.886446155245185	27.246338757314426	23.024425232201022
62-63	22.8624862416805	26.582467983410602	27.163612233723256	23.39143354118565
64-65	22.321159027844658	26.46267433567187	27.33613873543988	23.880027901043594
66-67	22.632412185920998	26.402516052682607	27.166880764990843	23.798190996405555
68-69	22.242376302554828	26.48221418049395	27.355043952415333	23.920365564535892
70-71	22.361585585560995	26.15691391353957	27.368566786136412	24.11293371476302
72-73	22.48442995835705	26.51911979275432	27.134005066490868	23.862445182397764
74-75	22.566009162697597	26.58896982097047	27.010396925111703	23.83462409122023
76-77	22.389516240271792	26.39407455986616	26.892004182298322	24.324405017563723
78-79	22.215828310212178	26.36769886642082	27.118789226984287	24.297683596382722
80-81	22.18536674080604	26.68651431728114	26.965846356630223	24.162272585282597
82-83	22.615077611260663	26.289515256656752	27.32369512567861	23.771712006403973
84-85	22.675655140875474	26.442084470946163	26.715674545443225	24.166585842735138
86-87	21.992899349732618	26.773247506796544	27.045459004492926	24.188394138977912
88-89	22.218145787082474	26.08243854112945	27.12111345650669	24.578302215281383
90-91	22.42972326222014	25.995477114439613	27.075409660986605	24.499389962353643
92-93	22.50031646530602	26.09971267338631	26.944453258842003	24.455517602465665
94-95	21.999492109831547	26.025046304246004	27.07812082684929	24.897340759073163
96-97	21.97772660791402	25.930453569831595	27.091747315501447	25.000072506752936
98-99	21.731015292857194	26.09047762029221	26.974900879629317	25.203606207221284
100	21.64858949619636	26.09839346707204	26.846274303610823	25.406742733120772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	572.0
1	394.0
2	323.5
3	440.5
4	379.5
5	398.5
6	611.5
7	1197.5
8	1980.0
9	2541.0
10	2518.0
11	2392.0
12	2640.5
13	2965.5
14	3368.5
15	3795.0
16	4313.0
17	4952.5
18	5637.5
19	6199.5
20	6852.5
21	7814.0
22	8974.0
23	10336.5
24	12242.5
25	14624.5
26	17885.5
27	22134.5
28	27385.0
29	35157.5
30	45709.5
31	57012.5
32	70889.0
33	85770.0
34	100140.0
35	114933.5
36	131988.0
37	153500.0
38	166549.5
39	170686.0
40	171359.5
41	170705.5
42	175579.0
43	184512.0
44	195182.5
45	214001.0
46	231915.0
47	244319.5
48	254940.5
49	259074.0
50	258074.0
51	257311.0
52	253680.0
53	244222.5
54	234248.0
55	212801.0
56	189233.5
57	165660.5
58	139590.0
59	119791.0
60	96552.5
61	73866.0
62	57015.0
63	43586.0
64	31562.0
65	21034.5
66	14532.5
67	10598.0
68	8042.0
69	6002.0
70	4357.0
71	3167.0
72	2319.0
73	1630.0
74	1174.0
75	854.5
76	522.0
77	283.5
78	171.5
79	99.5
80	62.5
81	31.0
82	14.5
83	11.5
84	11.5
85	8.0
86	3.5
87	1.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005220388243344474
2	0.03203885333660433
3	0.0013136271069853743
4	0.0014159876607764423
5	0.007028758026986677
6	3.412018459702271E-5
7	0.001108905999403238
8	0.0073187795960613705
9	0.0011430261840002607
10-11	2.6443143062692597E-4
12-13	7.677041534330109E-5
14-15	8.70064707224079E-4
16-17	8.530046149255676E-5
18-19	0.0010406656302091924
20-21	2.4737133832841463E-4
22-23	0.0017827796451944365
24-25	0.0012027365070450503
26-27	0.0021069213988661522
28-29	2.0472110758213625E-4
30-31	0.0019704406604780615
32-33	0.001740129414448158
34-35	7.591741072837553E-4
36-37	0.003966471459403889
38-39	9.809553071644028E-4
40-41	0.003829990721015799
42-43	0.004077362059344213
44-45	0.003727630167224731
46-47	0.002610194121672237
48-49	0.009289220256539431
50-51	0.006602255719523893
52-53	0.004444154043762207
54-55	0.0045891648282995546
56-57	0.003744690259523242
58-59	0.0055445299970161895
60-61	0.007506440611344995
62-63	0.003062286567582788
64-65	0.0034717287827470606
66-67	0.003915291182508356
68-69	0.0021069213988661522
70-71	0.0030196363368365092
72-73	0.00643165479653878
74-75	0.008137664026389916
76-77	0.004478274228359231
78-79	0.002635784260120004
80-81	0.002559013844776703
82-83	0.004665935243642855
84-85	0.002158101675761686
86-87	0.003377898275105248
88-89	5.118027689553406E-4
90-91	0.0018680801066869933
92-93	1.876610152836249E-4
94-95	0.00220928195265722
96-97	0.0018766101528362488
98-99	0.0012453867377913287
100	6.824036919404542E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5861633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.923882971577434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89229802389322	45.62729600280171
2	15.221854861195963	18.5474900834768
3	4.718047074006465	8.623252433734898
4	1.9703773320887918	4.8017215196010605
5	0.9624338107920165	2.931760242829106
6	0.5830324427718064	2.1312360187237473
7	0.35974300191620207	1.5341858384010654
8	0.24506098574555166	1.1944053453169114
9	0.17755742424796223	0.973573896205582
>10	0.8070877662101468	8.367366243448474
>50	0.04173168357361354	1.7451100122622565
>100	0.019346209267202683	2.1157353129320393
>500	0.0011504803499620842	0.47808919160931324
>1k	1.7431496313130823E-4	0.21902573359255
>5k	3.486299262566286E-5	0.15056957189920028
>10k+	6.972598525132572E-5	0.5591825531655022
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14165	0.24165620740841331	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12162	0.2074848425344951	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7089	0.120938994304147	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.7060092298511352E-5
7	0.0	0.0	0.0	1.7060092298511352E-5	3.4120184597022705E-5
8	1.7060092298511352E-5	0.0	0.0	1.7060092298511352E-5	3.4120184597022705E-5
9	1.7060092298511352E-5	0.0	0.0	1.7060092298511352E-5	5.1180276895534064E-5
10-11	2.559013844776703E-5	0.0	0.0	1.7060092298511352E-5	5.1180276895534064E-5
12-13	3.4120184597022705E-5	0.0	0.0	3.4120184597022705E-5	7.677041534330108E-5
14-15	3.4120184597022705E-5	0.0	0.0	4.265023074627839E-5	1.3648073838809082E-4
16-17	8.530046149255676E-5	0.0	0.0	5.1180276895534064E-5	1.7060092298511352E-4
18-19	8.530046149255676E-5	0.0	0.0	5.1180276895534064E-5	1.7913096913436922E-4
20-21	8.530046149255676E-5	0.0	0.0	1.0236055379106811E-4	1.876610152836249E-4
22-23	8.530046149255676E-5	0.0	0.0	2.3031124602990326E-4	2.1325115373139192E-4
24-25	8.530046149255676E-5	0.0	0.0	4.947426766568293E-4	2.3884129217915896E-4
26-27	1.3648073838809082E-4	0.0	0.0	0.0011515562301495164	2.4737133832841463E-4
28-29	1.3648073838809082E-4	0.0	0.0	0.00347172878274706	2.7296147677618164E-4
30-31	1.876610152836249E-4	3.4120184597022705E-5	0.0	0.014373127761495815	2.9002156907469303E-4
32-33	1.876610152836249E-4	3.4120184597022705E-5	0.0	0.044296529653084726	3.4120184597022705E-4
34-35	1.876610152836249E-4	3.4120184597022705E-5	0.0	0.09342959547279743	3.4120184597022705E-4
36-37	1.876610152836249E-4	3.4120184597022705E-5	0.0	0.17190602004594965	3.753220305672498E-4
38-39	1.876610152836249E-4	3.4120184597022705E-5	0.0	0.291855528996783	4.0944221516427245E-4
40-41	1.876610152836249E-4	3.4120184597022705E-5	0.0	0.42525692072499255	4.2650230746278385E-4
42-43	2.217811998806476E-4	3.4120184597022705E-5	0.0	0.5595113170681276	4.2650230746278385E-4
44-45	2.217811998806476E-4	3.4120184597022705E-5	0.0	0.7254548348557476	4.350323536120395E-4
46-47	2.217811998806476E-4	3.4120184597022705E-5	0.0	0.9224050703959119	4.606224920598065E-4
48-49	2.217811998806476E-4	3.4120184597022705E-5	0.0	1.1349652903892142	4.776825843583179E-4
50-51	2.217811998806476E-4	3.4120184597022705E-5	0.0	1.3578639263154142	4.862126305075736E-4
52-53	2.217811998806476E-4	3.4120184597022705E-5	0.0	1.5793380445346887	5.288628612538519E-4
54-55	2.217811998806476E-4	3.4120184597022705E-5	0.0	1.8330898573827463	5.885731842986417E-4
56-57	2.217811998806476E-4	5.1180276895534064E-5	0.0	2.120296511228185	6.141633227464087E-4
58-59	2.217811998806476E-4	5.1180276895534064E-5	0.0	2.4446429860074828	6.312234150449201E-4
60-61	2.217811998806476E-4	5.971032304478974E-5	0.0	2.78527331888571	6.312234150449201E-4
62-63	2.217811998806476E-4	7.677041534330108E-5	0.0	3.1430490445239405	6.482835073434314E-4
64-65	2.217811998806476E-4	8.530046149255676E-5	0.0	3.497259210871783	6.482835073434314E-4
66-67	2.217811998806476E-4	8.530046149255676E-5	0.0	3.8926439099820818	6.653435996419428E-4
68-69	2.3884129217915896E-4	8.530046149255676E-5	0.0	4.335430075543795	6.824036919404541E-4
70-71	2.3884129217915896E-4	1.2795069223883515E-4	0.0	4.813888894101694	6.824036919404541E-4
72-73	2.559013844776703E-4	1.5354083068660218E-4	0.0	5.290872014675774	7.847642457315222E-4
74-75	2.559013844776703E-4	1.5354083068660218E-4	0.0	5.759768651500359	8.103543841792893E-4
76-77	2.559013844776703E-4	1.5354083068660218E-4	0.0	6.272287944332237	8.274144764778006E-4
78-79	2.559013844776703E-4	1.5354083068660218E-4	0.0	6.834673886952663	8.359445226270564E-4
80-81	2.559013844776703E-4	1.5354083068660218E-4	0.0	7.424065955681634	8.530046149255677E-4
82-83	2.559013844776703E-4	1.5354083068660218E-4	0.0	8.025664520450189	8.871247995225904E-4
84-85	2.559013844776703E-4	1.5354083068660218E-4	0.0	8.626341840234623	8.871247995225904E-4
86-87	2.559013844776703E-4	1.5354083068660218E-4	0.0	9.241622599026584	9.127149379703574E-4
88	2.559013844776703E-4	1.5354083068660218E-4	0.0	9.723314305075053	9.383050764181244E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9040	0.0	49.98384	1
GTATCAA	16410	0.0	41.747036	1
CAACGCA	19885	0.0	33.374958	5
ATCAACG	20065	0.0	33.123253	3
TATCAAC	21170	0.0	32.071358	2
AACGCAG	21520	0.0	31.35997	6
TCAACGC	21380	0.0	31.327267	4
GTACATG	24440	0.0	27.74206	1
ACGCAGA	23890	0.0	27.639084	7
GTACTAG	3090	0.0	27.389791	1
TACATGG	24575	0.0	27.168509	2
CGCAGAG	24540	0.0	26.772482	8
ACATGGG	24620	0.0	26.498674	3
GTGGTAT	3755	0.0	26.045223	1
CATGGGG	19730	0.0	25.51395	4
ATGGGGG	14370	0.0	24.236673	5
GCAGAGT	27210	0.0	23.81685	9
CTGTGCG	3975	0.0	22.581089	9
TGGTATC	4170	0.0	22.438198	2
CTAACGC	1470	0.0	22.382236	3
>>END_MODULE