FastQCFastQC Report
Thu 26 May 2016
ERR580102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7351834
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173490.23598193321557587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132590.18034955631479166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.10742897622552414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109100.048.919371
GTATCAA198950.040.7004241
CAACGCA232950.033.6546365
ATCAACG234900.033.4139063
TATCAAC248050.032.4451682
AACGCAG256000.031.051516
TCAACGC261550.030.3162064
ACGCAGA277000.028.1043747
CGCAGAG282950.027.264698
GTACTAG44400.026.6886941
GTACATG278750.026.5690141
TACATGG284050.025.4369222
ACATGGG278150.024.9740623
GCATCGT40650.024.7337728
GCAGAGT313500.024.2074459
TAGTACT57850.024.1305714
CTGTGCG53250.023.7381369
GTGGTAT47200.023.2125911
CATGGGG211150.023.0612664
CTAGTAC60850.022.2446563
Produced by FastQC (version 0.11.5)