FastQCFastQC Report
Thu 26 May 2016
ERR580102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7351834
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159010.21628616750595836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130390.17735710572355143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76650.10425969900843789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106700.047.6328161
GTATCAA194750.041.5478321
ATCAACG232850.033.5278243
CAACGCA232950.033.45295
TATCAAC251850.031.773752
AACGCAG261650.030.4285356
GTACTAG43000.030.3963381
TCAACGC259250.030.3490944
ACGCAGA281200.027.5943497
CGCAGAG287000.026.922068
GTGGTAT42500.025.775791
GTACATG279900.025.4984531
TACATGG284650.024.6273292
TAGGCAT58300.024.2666575
ACATGGG279350.024.1275083
GCAGAGT320250.023.8912499
GCATCGT48450.023.3782318
CTGTGCG53400.021.9145079
CTAGGCA64650.021.8830074
CATGGGG208150.021.7449744
Produced by FastQC (version 0.11.5)