FastQCFastQC Report
Thu 26 May 2016
ERR580101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5548304
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130590.23536922273905683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108100.19483431333250667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.11446741202356613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79750.049.8842621
GTATCAA137000.039.3701171
CAACGCA159800.032.0030825
ATCAACG163800.031.3352173
TATCAAC174250.030.6492372
AACGCAG178250.029.1262366
GTACTAG30200.028.650671
TCAACGC180000.028.5936454
GTACATG188650.026.1981011
TACATGG186350.025.909272
ACGCAGA196750.025.886547
CGCAGAG199300.025.4617168
GTGGTAT33350.025.0985161
ACATGGG188750.024.5286273
GCATCGT32200.024.0768288
CATGGGG148750.023.3831314
TAGGCAT41150.023.1881375
CTGTGCG37800.022.5009619
GCAGAGT222050.022.326269
CTAGGCA47650.021.8000374
Produced by FastQC (version 0.11.5)