FastQCFastQC Report
Thu 26 May 2016
ERR580101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5548304
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115950.20898278104444168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101930.1837137979461832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57180.10305852022527967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.050.5158651
GTATCAA147250.040.8645671
CAACGCA169600.034.2246785
ATCAACG171050.033.9901243
TATCAAC184750.032.1125642
AACGCAG186200.031.854456
TCAACGC187450.031.191284
ACGCAGA204150.028.2248427
CGCAGAG207650.027.4998868
GTACATG189000.026.9873451
GTGGTAT30300.026.065071
TACATGG190600.026.021342
ACATGGG194450.024.7754763
CTGTGCG37300.024.6946289
GCAGAGT235550.023.8817569
GCATCGT35400.023.2338588
TAGGCAT41250.023.1297195
TGGTATC34800.022.8300842
TAGTACT43950.022.457364
CATGGGG149200.022.2084674
Produced by FastQC (version 0.11.5)