FastQCFastQC Report
Thu 26 May 2016
ERR580100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184181
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43950.040.6580851
GGTATCA24700.038.2667241
CAACGCA50300.033.838655
ATCAACG52700.032.4782833
TATCAAC54650.032.009942
GTACTAG10450.031.499521
AACGCAG57100.030.6963276
TCAACGC55750.030.5320554
GTACATG93950.028.8302141
TACATGG96600.027.6018432
ACATGGG100200.026.1390483
ACGCAGA67050.025.2995157
CATGGGG82300.025.024574
ATGGGGG57700.024.283595
CGCAGAG70300.023.9962238
CGTTAAC3500.022.8403681
TAGGCAT16300.022.7882985
GCATCGT13100.022.5981568
CTAGGCA17300.022.5592334
TGGGGGG50450.021.7024356
Produced by FastQC (version 0.11.5)