FastQCFastQC Report
Thu 26 May 2016
ERR580100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184181
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42300.042.3622971
GGTATCA24050.039.0188331
CAACGCA47000.036.9986655
ATCAACG48650.035.456453
TATCAAC52500.033.8411032
AACGCAG54450.032.8836066
TCAACGC52950.032.753864
ACGCAGA61650.028.204677
GTACATG91750.027.4455181
TACATGG93400.026.8220772
CGCAGAG66500.025.864968
ACATGGG98450.025.016563
GTACTAG8400.024.7007221
CATGGGG80900.023.5873744
GCAGAGT74850.022.5405739
ATGGGGG54250.022.3512695
TGGGGGG47650.021.796256
GAGTACG2109.237574E-720.209681
TATGTAA5900.019.9159052
CGTTAAC3850.019.5972671
Produced by FastQC (version 0.11.5)