##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580100_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2184181
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.519777893865026	34.0	31.0	34.0	31.0	34.0
2	32.72252253819624	34.0	31.0	34.0	31.0	34.0
3	32.80689558237161	34.0	31.0	34.0	31.0	34.0
4	36.27490075227282	37.0	37.0	37.0	35.0	37.0
5	36.19810446112296	37.0	37.0	37.0	35.0	37.0
6	36.22348056319508	37.0	37.0	37.0	35.0	37.0
7	36.18983728912576	37.0	37.0	37.0	35.0	37.0
8	36.179703971419954	37.0	37.0	37.0	35.0	37.0
9	37.965305988835176	39.0	38.0	39.0	35.0	39.0
10-11	37.910776396278514	39.0	38.0	39.0	35.0	39.0
12-13	37.86522293711006	39.0	38.0	39.0	35.0	39.0
14-15	39.27031528064753	41.0	39.0	41.0	36.0	41.0
16-17	39.244429147584384	41.0	39.0	41.0	36.0	41.0
18-19	39.220633500611896	41.0	39.0	41.0	36.0	41.0
20-21	39.197671575753105	41.0	39.0	41.0	36.0	41.0
22-23	39.12845249546626	40.0	39.0	41.0	35.0	41.0
24-25	39.04355293814936	40.0	38.5	41.0	35.0	41.0
26-27	38.90881318901684	40.0	38.0	41.0	35.0	41.0
28-29	38.79322936148607	40.0	38.0	41.0	35.0	41.0
30-31	38.697427319439186	40.0	38.0	41.0	35.0	41.0
32-33	38.57822909365112	40.0	38.0	41.0	34.0	41.0
34-35	38.56626099210642	40.0	38.0	41.0	34.5	41.0
36-37	38.639789925834904	40.0	38.0	41.0	35.0	41.0
38-39	38.55795810878311	40.0	38.0	41.0	34.0	41.0
40-41	38.44222411054761	40.0	38.0	41.0	34.0	41.0
42-43	38.424001032881435	40.0	38.0	41.0	34.0	41.0
44-45	38.357528290924606	40.0	38.0	41.0	34.0	41.0
46-47	38.194508376366244	40.0	37.5	41.0	33.5	41.0
48-49	38.0155161133624	40.0	37.0	41.0	33.0	41.0
50-51	37.83811666707109	40.0	37.0	41.0	33.0	41.0
52-53	37.64531259085213	40.0	36.0	41.0	33.0	41.0
54-55	37.41845387355718	39.0	36.0	41.0	33.0	41.0
56-57	37.17378046965888	39.0	35.0	41.0	32.0	41.0
58-59	36.90923096574872	39.0	35.0	40.5	32.0	41.0
60-61	36.601349659208644	38.0	35.0	40.0	31.5	41.0
62-63	36.26345138063191	37.0	35.0	40.0	31.0	41.0
64-65	35.93445506576607	37.0	35.0	39.5	31.0	41.0
66-67	35.57016245448523	36.0	35.0	39.0	31.0	41.0
68-69	35.17957074070327	36.0	34.0	39.0	30.0	40.5
70-71	34.83043667168609	35.0	34.0	37.5	30.0	40.0
72-73	34.467634321514566	35.0	34.0	37.0	30.0	39.0
74-75	34.12583320704648	35.0	34.0	36.5	29.0	39.0
76-77	32.935323354612095	34.5	32.0	35.0	28.0	37.0
78-79	33.57826434713973	35.0	33.5	36.0	29.0	37.0
80-81	33.54611110526096	35.0	34.0	35.0	29.0	37.0
82-83	33.389926475873565	35.0	34.0	35.0	29.0	36.0
84-85	33.226715871990464	35.0	34.0	35.0	29.0	36.0
86-87	33.104500725901374	35.0	33.0	35.0	29.0	36.0
88-89	32.98942647152411	35.0	33.0	35.0	29.0	35.0
90-91	32.86390573858119	35.0	33.0	35.0	29.0	35.0
92-93	32.77334341796765	35.0	33.0	35.0	29.0	35.0
94-95	32.69765555144011	35.0	33.0	35.0	29.0	35.0
96-97	32.62145994310911	35.0	33.0	35.0	28.0	35.0
98-99	32.557988783896576	35.0	33.0	35.0	27.0	35.0
100	31.2542554852368	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.09053257530385395
1101	2	0.05797895195624392
1101	3	-4.6910363283103607E-4
1101	4	-0.01564593261330316
1101	5	-0.07807324440100416
1101	6	-0.013587299537064723
1101	7	-0.08465726209502833
1101	8	0.0051431191041189095
1101	9	-0.019013537323921526
1101	10-11	-0.05433615893967669
1101	12-13	-0.09408303507986204
1101	14-15	-0.062196074174146077
1101	16-17	-0.011503864312714995
1101	18-19	-0.007553483789138227
1101	20-21	-0.10651042661221055
1101	22-23	-0.15091214314648482
1101	24-25	-0.1587705228425449
1101	26-27	-0.19921013904651375
1101	28-29	-0.17679296926825572
1101	30-31	-0.1738867013843972
1101	32-33	-0.16130895190865147
1101	34-35	-0.21458332377684286
1101	36-37	-0.11333576262295253
1101	38-39	-0.024974811237150618
1101	40-41	-0.06313770916329275
1101	42-43	-0.068648725054409
1101	44-45	-0.07434998308188767
1101	46-47	-0.0016206793660913377
1101	48-49	-0.12641183232279474
1101	50-51	-0.03978047412738306
1101	52-53	-0.10967302547223312
1101	54-55	-0.18247001399828378
1101	56-57	-0.2572877415204786
1101	58-59	-0.12107917783885824
1101	60-61	-0.1350324910367604
1101	62-63	-0.1814400724686962
1101	64-65	-0.21019428401039875
1101	66-67	-0.18892862105689545
1101	68-69	-0.1945290721325179
1101	70-71	-0.24327158382332215
1101	72-73	-0.11347847497129493
1101	74-75	-0.09744225932772821
1101	76-77	-0.1399232625462048
1101	78-79	-0.2367375128702207
1101	80-81	-0.2095363738883833
1101	82-83	-0.05037832056297731
1101	84-85	-0.2765015878024215
1101	86-87	-0.35055937983553775
1101	88-89	-0.24784754418386257
1101	90-91	-0.22241727055344995
1101	92-93	-0.2963217574639998
1101	94-95	-0.3358004288640899
1101	96-97	-0.39830414813262394
1101	98-99	-0.44107725152152
1101	100	-0.5036046005625643
1102	1	0.08254809585272227
1102	2	0.023331238434643353
1102	3	0.005166437207456909
1102	4	0.001679988050277359
1102	5	-0.00968919337263685
1102	6	-0.04478851267739259
1102	7	-0.00846709626587483
1102	8	0.026140179672054842
1102	9	-0.0715120545727217
1102	10-11	0.03952818398971658
1102	12-13	-0.04324867522646514
1102	14-15	-0.028897175429214883
1102	16-17	-0.07787495837671088
1102	18-19	-0.03182020085848336
1102	20-21	-0.04983174585886729
1102	22-23	-0.013462192788779248
1102	24-25	-0.0391095677552471
1102	26-27	-0.042450531579099504
1102	28-29	-0.034133836932980444
1102	30-31	-0.08929112856447574
1102	32-33	-0.12718970612390024
1102	34-35	-0.15940857638767625
1102	36-37	-0.05320721406800999
1102	38-39	-0.14527850909917106
1102	40-41	-0.046938244909007665
1102	42-43	-0.062185776454271036
1102	44-45	-0.10340767025071074
1102	46-47	-0.05749096199041759
1102	48-49	-0.07299759400067529
1102	50-51	-0.06706939661805222
1102	52-53	-0.043235777892981275
1102	54-55	-0.1105849321047927
1102	56-57	-0.10381864683513697
1102	58-59	-0.16335551052259945
1102	60-61	-0.2254538096316523
1102	62-63	-0.07979535920996028
1102	64-65	-0.03831930437785758
1102	66-67	-0.13384522852214076
1102	68-69	-0.1205863877988449
1102	70-71	-0.06390713552626437
1102	72-73	-0.10511738757696065
1102	74-75	-0.07587773412313226
1102	76-77	-0.20206270416274208
1102	78-79	-0.10067787259761474
1102	80-81	-0.06333667065587179
1102	82-83	-0.057286529030200484
1102	84-85	-0.11354994613069636
1102	86-87	-0.01656342508497488
1102	88-89	-0.034342450689074155
1102	90-91	-0.11511224962113431
1102	92-93	-0.09360348888289849
1102	94-95	-0.1805405183288471
1102	96-97	-0.1218252531678985
1102	98-99	-0.1173143439821942
1102	100	0.03507272018747898
1103	1	0.1442135542896139
1103	2	0.07189905464563395
1103	3	0.043603246790446804
1103	4	0.0225780479784774
1103	5	0.06909127127801185
1103	6	0.061244868985134815
1103	7	0.05983283814450857
1103	8	0.023171818031627822
1103	9	0.0029908451787292734
1103	10-11	0.009409604867933297
1103	12-13	0.06331352271609347
1103	14-15	0.04276271652052088
1103	16-17	0.010807812266762085
1103	18-19	0.03584994378388018
1103	20-21	-0.04990143482600473
1103	22-23	-0.10502335510582128
1103	24-25	-0.039628675046053274
1103	26-27	-0.06207585089696721
1103	28-29	-0.004746884088461911
1103	30-31	0.03551900675726216
1103	32-33	-0.23030370260277522
1103	34-35	-0.1590187065312847
1103	36-37	-0.15620232936765177
1103	38-39	-0.14761470766309515
1103	40-41	-0.03973093857097609
1103	42-43	-0.12297289499887398
1103	44-45	-0.10199921954648516
1103	46-47	-0.05235663273054314
1103	48-49	-0.1125966774720979
1103	50-51	-0.0942547822159554
1103	52-53	-0.0997876195803542
1103	54-55	-0.11419926516195744
1103	56-57	-0.10803989959680393
1103	58-59	-0.14329026611498108
1103	60-61	-0.1733914045639935
1103	62-63	-0.20911068128950205
1103	64-65	-0.14973125031820445
1103	66-67	-0.06472046153788114
1103	68-69	-0.1351343372603182
1103	70-71	-0.053810218238218965
1103	72-73	-0.18251809698044497
1103	74-75	-0.144984161905505
1103	76-77	-0.2192808414176426
1103	78-79	-0.06232909118364205
1103	80-81	-0.04675738489227399
1103	82-83	-0.07472430519700168
1103	84-85	-0.0652498822671248
1103	86-87	-0.009028170666304902
1103	88-89	-0.0828731569856771
1103	90-91	-0.10061780747808058
1103	92-93	-0.21987709251449417
1103	94-95	-0.20501968499551282
1103	96-97	-0.13214547720821912
1103	98-99	-0.032184416130768057
1103	100	-0.0023703204090423924
1104	1	0.031616701032184835
1104	2	0.021049447329588133
1104	3	0.007475993469761022
1104	4	0.004917152975309591
1104	5	0.01853156486856733
1104	6	-0.0032745543756078632
1104	7	0.07908654096972612
1104	8	0.027047595493151277
1104	9	-0.040441581555406
1104	10-11	0.029877267739529145
1104	12-13	0.03608836854152031
1104	14-15	-0.006962701200329491
1104	16-17	-0.0477175804171992
1104	18-19	0.01150732703288071
1104	20-21	-0.028214046833170414
1104	22-23	-0.0453227107351708
1104	24-25	-4.838168339489357E-4
1104	26-27	-0.05747779191531066
1104	28-29	-0.08705485768113164
1104	30-31	-0.0706506116277339
1104	32-33	-0.08739655654542844
1104	34-35	-0.08783568022936805
1104	36-37	-0.15021488734783617
1104	38-39	-0.06069385061060473
1104	40-41	-0.02745215272867796
1104	42-43	-0.06827139410281546
1104	44-45	-0.1277608682920004
1104	46-47	-0.06243315998123222
1104	48-49	0.004588351781542599
1104	50-51	-0.05231221553874832
1104	52-53	-0.18821974982492407
1104	54-55	-0.1505686516262159
1104	56-57	-0.1121355734846432
1104	58-59	-0.14303330849322293
1104	60-61	-0.048556944570322
1104	62-63	-0.07065746734263456
1104	64-65	-0.043862239407395975
1104	66-67	-0.12310831600762384
1104	68-69	-0.08984720358964893
1104	70-71	-0.04091127505879655
1104	72-73	-0.18146967694038096
1104	74-75	-0.2594653710558319
1104	76-77	-0.16492112710180606
1104	78-79	-0.08535150579464812
1104	80-81	-0.099628886315422
1104	82-83	-0.2168465774930155
1104	84-85	-0.09360004961902035
1104	86-87	-0.03856100194397527
1104	88-89	-0.15628891441767934
1104	90-91	-0.1638590313869983
1104	92-93	-0.11145332986427547
1104	94-95	-0.12462653983422456
1104	96-97	-0.16949739714286238
1104	98-99	-0.08658778029901981
1104	100	-0.0979387897322539
1105	1	0.12886906152637323
1105	2	0.10531281941790382
1105	3	0.05887408565646979
1105	4	0.018770037754066493
1105	5	-0.025871009627579156
1105	6	0.026415082104236376
1105	7	0.020208493767931657
1105	8	0.024118115004142737
1105	9	0.0031867023026208585
1105	10-11	-6.301474889980341E-4
1105	12-13	0.03936241645530458
1105	14-15	0.09098910992777576
1105	16-17	0.06934523259606351
1105	18-19	-0.00303651302795771
1105	20-21	0.013016525106273491
1105	22-23	-0.0679440122230659
1105	24-25	-0.0823327897054611
1105	26-27	0.007496479304869297
1105	28-29	-0.004269171135334204
1105	30-31	-0.1202521391728979
1105	32-33	-0.12274338939735685
1105	34-35	-0.01653773205909914
1105	36-37	-0.004983409237517833
1105	38-39	0.02702884896948632
1105	40-41	0.018785215492229668
1105	42-43	0.06645113152482907
1105	44-45	-0.02761998704084334
1105	46-47	4.356798773414994E-4
1105	48-49	0.01269534466971578
1105	50-51	0.05171842436351426
1105	52-53	0.008041789694644308
1105	54-55	-0.014702186420755936
1105	56-57	0.020160517844438175
1105	58-59	7.750970505995269E-4
1105	60-61	0.019329411894645432
1105	62-63	-0.04503396919947278
1105	64-65	-0.12796258164870977
1105	66-67	0.012605030594635025
1105	68-69	-0.03702361159307799
1105	70-71	-0.07687675156712714
1105	72-73	0.04799837244592453
1105	74-75	0.013111127564684466
1105	76-77	0.013550538877133533
1105	78-79	0.0901454722873467
1105	80-81	-0.07375962978007067
1105	82-83	0.06004303147808088
1105	84-85	0.1609527510153086
1105	86-87	0.03193221391077117
1105	88-89	0.025670902568165843
1105	90-91	0.05633138585614006
1105	92-93	-0.004792670624965467
1105	94-95	-0.03648483736571251
1105	96-97	-0.038719243142352866
1105	98-99	-0.015477741220401242
1105	100	0.002160120435469537
1106	1	0.1540395519118647
1106	2	0.13057316686521148
1106	3	0.027318388163337204
1106	4	0.040890942116796225
1106	5	0.050850258924036496
1106	6	0.05000385016987252
1106	7	0.10858241816104908
1106	8	0.0885204366091088
1106	9	0.023059593240347454
1106	10-11	0.013523827446242365
1106	12-13	-0.06583421777290255
1106	14-15	-0.03071137050005035
1106	16-17	0.014441476848588763
1106	18-19	-0.00246345110394941
1106	20-21	-0.030988045107200435
1106	22-23	0.0029604496858723905
1106	24-25	0.022880209219430014
1106	26-27	-0.08705477095795544
1106	28-29	-0.044753943041222044
1106	30-31	-0.07681041534549848
1106	32-33	-0.027528823428788485
1106	34-35	0.050340221024804066
1106	36-37	0.021493479843549324
1106	38-39	-0.05607931274941791
1106	40-41	0.04596628243078271
1106	42-43	0.026061869939617566
1106	44-45	-0.014125757332074329
1106	46-47	-0.047422230916218666
1106	48-49	-0.08600162875401196
1106	50-51	0.008648030380967953
1106	52-53	-0.12054812885876487
1106	54-55	-0.09542923518159796
1106	56-57	-0.1772414625813994
1106	58-59	-0.0724002888586952
1106	60-61	-0.07041428720123122
1106	62-63	-0.11434262523140148
1106	64-65	-0.030631614088498793
1106	66-67	0.020923453870992148
1106	68-69	-0.08826256790231213
1106	70-71	-0.030594017214752967
1106	72-73	-0.07178178521056822
1106	74-75	-0.20441020604899762
1106	76-77	-0.20256065378195842
1106	78-79	-0.0988797670339494
1106	80-81	0.015970779198497098
1106	82-83	0.028199910403124306
1106	84-85	-0.022441049045788475
1106	86-87	-0.07017655082240992
1106	88-89	-0.018257894140639053
1106	90-91	-0.07986900927689788
1106	92-93	-0.09503256716041619
1106	94-95	-0.1387713326229303
1106	96-97	-0.013460978474626017
1106	98-99	-1.3281308621770904E-4
1106	100	0.06680741659085498
1107	1	0.12022537425073665
1107	2	0.046448150257170084
1107	3	0.04040214601288028
1107	4	-0.005794768440985365
1107	5	0.018049868127796742
1107	6	0.014874156259118365
1107	7	0.017612301502964556
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2316	8	0.06523560380844629
2316	9	0.06497258289241614
2316	10-11	0.06163136945222192
2316	12-13	0.05974600499486371
2316	14-15	0.12879758067804659
2316	16-17	0.13655879863691212
2316	18-19	0.13174736964653988
2316	20-21	0.14827091170148776
2316	22-23	0.13560660334200492
2316	24-25	0.0639023857350196
2316	26-27	0.18390372181782055
2316	28-29	0.23226169763587734
2316	30-31	-0.18287003343922237
2316	32-33	-0.020540176868998117
2316	34-35	-0.0939020329240563
2316	36-37	-0.3734744839405195
2316	38-39	-0.3113608928879117
2316	40-41	-0.17423738493310736
2316	42-43	-0.25988542341971055
2316	44-45	-0.10747668957542089
2316	46-47	-0.3012918962995954
2316	48-49	-0.2976137489710169
2316	50-51	-0.3357791610611258
2316	52-53	-0.2679685759490482
2316	54-55	-0.25779743406986455
2316	56-57	-0.9403266711419249
2316	58-59	-0.7360022217116082
2316	60-61	-0.33898731880298527
2316	62-63	-0.15202023388172137
2316	64-65	8.276484428293429E-4
2316	66-67	0.04962599984018112
2316	68-69	-0.062375859644383524
2316	70-71	-0.16935326136493956
2316	72-73	0.012416630088353031
2316	74-75	-0.04050149709505746
2316	76-77	-0.14650944524032639
2316	78-79	-0.11634938548367302
2316	80-81	-0.13034589147618192
2316	82-83	-0.3125122751286682
2316	84-85	-0.14493489354426714
2316	86-87	-0.23846476560709107
2316	88-89	-0.3203860375512946
2316	90-91	-0.17127511907985848
2316	92-93	-0.16903371943251955
2316	94-95	-0.07670284435888419
2316	96-97	-0.2250615650726786
2316	98-99	-0.056656445925533205
2316	100	-0.41373455088833566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	21.0
14	81.0
15	210.0
16	487.0
17	965.0
18	1713.0
19	2566.0
20	3619.0
21	5061.0
22	6761.0
23	7955.0
24	9306.0
25	10898.0
26	13619.0
27	17403.0
28	21904.0
29	27013.0
30	32952.0
31	41517.0
32	52641.0
33	69824.0
34	106481.0
35	158992.0
36	295814.0
37	625220.0
38	607831.0
39	63318.0
40	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.541767750283036	18.34272492023584	13.324244997279934	24.791262332201196
2	16.778715537189022	20.03551927829661	37.54380390501454	25.64196127949982
3	20.013561438995957	24.573126007836514	28.560976570118868	26.852335983048658
4	14.40608125617516	16.192585321364703	35.889227591094766	33.51210583136537
5	15.295114167586204	36.8979016240322	32.27291249822579	15.53407171015581
6	32.86472137611306	34.11850940924768	18.025658129980986	14.991111084658277
7	29.66075582633108	30.96861566748879	20.566904086157773	18.803724420022352
8	28.402067236463065	32.543453537541914	19.324439650357206	19.730039575637818
9	27.57966749827962	15.70457335233727	19.12065743971569	37.595101709667425
10-11	26.97710971099216	24.47596454412286	26.550473530220387	21.99645221466459
12-13	27.57535377685132	21.66082427711446	26.577176199494502	24.18664574653972
14-15	24.977691805830577	23.0988588368606	24.09568830336397	27.827761053944855
16-17	25.020808743609724	25.874974475523516	24.482368195266137	24.621848585600624
18-19	24.636937000251127	25.690843548742663	25.8868228258971	23.785396625109108
20-21	25.71256881889043	24.937470669703664	25.368915036569874	23.981045474836034
22-23	25.49592398613576	24.52604077364485	25.261213530658296	24.716821709561092
24-25	25.08313184667388	24.613047178782345	25.48989758632641	24.81392338821737
26-27	24.929938002973255	24.909678284832864	25.259793685103087	24.90059002709079
28-29	24.787767534247955	25.24073617878994	25.364252268620824	24.60724401834128
30-31	25.543769893741324	24.691452421679553	25.557230791207747	24.20754689337138
32-33	25.312906100238013	24.872474794883605	25.113513459060517	24.70110564581786
34-35	25.320171240301683	25.101965545432964	25.426423745798033	24.15143946846732
36-37	25.208466980689302	24.912141888046214	25.112057727415717	24.767333403848767
38-39	25.12965230379529	25.020902799464086	25.149936948182123	24.69950794855851
40-41	25.18237538225967	24.435244748072073	25.496883197104616	24.88549667256364
42-43	24.8845495578406	24.614066318873068	25.32439674110622	25.176987382180112
44-45	24.82990557673461	24.82491508727977	25.424094312009522	24.921085023976097
46-47	24.744632877782973	24.57721543097573	25.040709705289892	25.637441985951405
48-49	25.03761584673431	24.71899153815158	25.3069709513169	24.93642166379721
50-51	24.939952713047685	24.57541096793571	25.421704564601082	25.062931754415523
52-53	24.85606603620893	24.711153397010953	25.171073605776726	25.261706961003387
54-55	24.49708893980268	24.52080597632692	25.55936493285057	25.422740151019834
56-57	24.985675385814567	24.63764964156988	25.01621319695858	25.36046177565697
58-59	24.64204889095019	24.876966825338904	25.386611921158043	25.094372362552864
60-61	25.003725667272157	24.888464624474484	25.221634280259458	24.886175427993898
62-63	25.04252208744876	24.819438511573562	24.984742411152304	25.153296989825375
64-65	24.56176777205835	24.56586543282057	25.374569688390068	25.497797106731014
66-67	24.74790021770433	24.70559552778992	25.274923254073084	25.271581000432665
68-69	24.340046320089353	24.79752416215099	25.417055929550607	25.445373588209048
70-71	24.388221585823416	24.459186920402075	25.384139934482974	25.768451559291538
72-73	24.31970873635274	24.828971429460957	25.239453734150498	25.6118661000358
74-75	24.468630653375623	24.87702761916405	25.186158412876765	25.468183314583566
76-77	24.205794061715142	24.675389190727323	24.972923822023148	26.145892925534387
78-79	24.16708616537887	24.768348576073716	25.181941112682278	25.88262414586514
80-81	24.278192631525936	25.126844522175475	24.929034827875117	25.66592801842347
82-83	24.455658937975507	24.835183953685082	25.412632444020687	25.29652466431872
84-85	24.416386771616626	25.003685640926086	24.981938070244194	25.597989517213087
86-87	23.914586755338327	25.18454503310879	25.240495172469053	25.66037303908383
88-89	24.00572305015681	24.601652816885284	25.363573930361927	26.029050202595975
90-91	24.19666057666048	24.742769538858873	25.191221692173194	25.869348192307456
92-93	24.277571505718374	24.718293463776533	25.229294686910453	25.77484034359464
94-95	23.812232758379924	24.80547076418455	25.429154730122306	25.953141747313218
96-97	23.702428521883252	24.836028702274	25.336750379724815	26.124792396117936
98-99	23.490722439622715	24.730775165156462	25.25766422589018	26.52083816933064
100	23.639588542293822	24.77083626164476	25.123195110929398	26.466380085132023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	21.5
2	49.0
3	74.5
4	51.5
5	49.0
6	95.0
7	157.5
8	236.5
9	275.5
10	258.0
11	243.5
12	272.5
13	322.0
14	355.5
15	388.5
16	457.5
17	512.0
18	565.0
19	637.0
20	688.0
21	740.0
22	829.0
23	941.0
24	1067.0
25	1245.5
26	1483.5
27	1779.0
28	2145.5
29	2586.0
30	3258.5
31	4097.5
32	5170.5
33	6414.0
34	8013.0
35	10336.5
36	13496.5
37	17533.0
38	22355.5
39	28776.0
40	35109.0
41	42230.5
42	51105.0
43	61006.0
44	72323.5
45	85521.5
46	98329.5
47	109449.0
48	119155.0
49	125253.0
50	126879.5
51	126794.5
52	125427.5
53	122607.5
54	118735.5
55	109376.5
56	98097.0
57	85052.0
58	71976.0
59	61798.5
60	49935.0
61	39014.5
62	30744.5
63	23283.0
64	16364.5
65	11104.5
66	7775.0
67	5636.5
68	4283.5
69	3177.5
70	2384.0
71	1874.5
72	1371.0
73	973.0
74	726.5
75	514.5
76	324.5
77	178.0
78	118.5
79	80.0
80	43.5
81	20.0
82	14.0
83	8.5
84	7.5
85	5.5
86	2.0
87	0.5
88	0.0
89	0.0
90	1.0
91	1.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35551082991748395
2	6.867562715727314E-4
3	0.0021518363175945586
4	0.0012361612888309164
5	0.005081996409638212
6	0.0
7	9.156750287636418E-5
8	4.120537629436389E-4
9	0.003296430103549111
10-11	6.867562715727314E-5
12-13	2.2891875719091045E-5
14-15	2.7470250862909255E-4
16-17	1.3735125431454628E-4
18-19	9.84350655920915E-4
20-21	2.7470250862909255E-4
22-23	2.2891875719091045E-5
24-25	0.0
26-27	0.002014485063280012
28-29	0.011674856616736434
30-31	0.003525348860740021
32-33	0.013689341680016445
34-35	0.010644722209377337
36-37	0.011995342876803707
38-39	0.01204112662824189
40-41	0.013391747295668263
42-43	0.005814536432649126
44-45	0.0012132694131118255
46-47	0.00547115829686276
48-49	0.011949559125365525
50-51	0.0031590788492345642
52-53	0.0048759695281663925
54-55	0.004441023889503663
56-57	1.6024313003363733E-4
58-59	6.409725201345493E-4
60-61	3.891618872245478E-4
62-63	0.001007242531640006
64-65	3.662700115054567E-4
66-67	0.0011903775373927343
68-69	0.001442188170302736
70-71	7.325400230109134E-4
72-73	0.00196870131184183
74-75	0.007050697721480042
76-77	0.0029988357192009267
78-79	0.0012132694131118255
80-81	5.03621265820003E-4
82-83	1.6024313003363733E-4
84-85	0.0013277287917072805
86-87	0.004280780759470026
88-89	0.001419296294583645
90-91	6.867562715727314E-5
92-93	0.0017168906789318285
94-95	0.00283859258916729
96-97	0.0015337556731791
98-99	0.0018313500575272838
100	0.002014485063280012
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2184181.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.00929684099339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21356444503178	54.4206384741228
2	11.22905867219579	15.049026516201977
3	3.268954325869157	6.571509923454473
4	1.4198908339878695	3.8058354550599525
5	0.795678227548547	2.6658919269858035
6	0.47953027098308393	1.9279791763528484
7	0.3322161712980129	1.55831004265208
8	0.2273008461593411	1.2185015898000207
9	0.17548468879939233	1.0583195042527047
>10	0.8225316578174758	9.19929981460353
>50	0.026706802852682003	1.2138085330292285
>100	0.008632979453669833	0.9817896089847813
>500	1.500260010957766E-4	0.06393795031063
>1k	3.0005200219154006E-4	0.26515148418912016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.156750287636418E-5
2	0.0	0.0	0.0	0.0	9.156750287636418E-5
3	0.0	0.0	0.0	0.0	9.156750287636418E-5
4	0.0	0.0	0.0	0.0	9.156750287636418E-5
5	0.0	0.0	0.0	4.578375143818209E-5	9.156750287636418E-5
6	0.0	0.0	0.0	4.578375143818209E-5	1.8313500575272836E-4
7	0.0	0.0	0.0	4.578375143818209E-5	1.8313500575272836E-4
8	0.0	0.0	0.0	4.578375143818209E-5	1.8313500575272836E-4
9	0.0	0.0	0.0	4.578375143818209E-5	1.8313500575272836E-4
10-11	0.0	0.0	0.0	4.578375143818209E-5	1.8313500575272836E-4
12-13	0.0	0.0	0.0	4.578375143818209E-5	1.8313500575272836E-4
14-15	0.0	0.0	0.0	4.578375143818209E-5	2.2891875719091047E-4
16-17	0.0	0.0	0.0	4.578375143818209E-5	2.2891875719091047E-4
18-19	0.0	0.0	0.0	9.156750287636418E-5	2.518106329100015E-4
20-21	0.0	0.0	0.0	1.3735125431454628E-4	2.7470250862909255E-4
22-23	0.0	0.0	0.0	3.2048626006727466E-4	2.7470250862909255E-4
24-25	0.0	0.0	0.0	6.180806444154583E-4	2.7470250862909255E-4
26-27	0.0	0.0	0.0	0.0011674856616736434	2.7470250862909255E-4
28-29	0.0	0.0	0.0	0.0022205119447518316	2.7470250862909255E-4
30-31	0.0	0.0	0.0	0.00679888708857004	2.7470250862909255E-4
32-33	0.0	0.0	0.0	0.019572553739822845	2.7470250862909255E-4
34-35	0.0	0.0	0.0	0.042372861956037525	2.7470250862909255E-4
36-37	0.0	0.0	0.0	0.07893118747942593	2.7470250862909255E-4
38-39	0.0	0.0	0.0	0.14067057629381446	2.7470250862909255E-4
40-41	0.0	0.0	0.0	0.2185487374901622	2.7470250862909255E-4
42-43	0.0	0.0	0.0	0.2959919530478472	2.7470250862909255E-4
44-45	0.0	0.0	0.0	0.39094745353063687	2.7470250862909255E-4
46-47	0.0	0.0	0.0	0.5030489689270258	2.7470250862909255E-4
48-49	0.0	0.0	0.0	0.6257036390299155	2.7470250862909255E-4
50-51	0.0	0.0	0.0	0.7592548419750927	2.7470250862909255E-4
52-53	0.0	0.0	0.0	0.8989868513644245	2.7470250862909255E-4
54-55	0.0	0.0	0.0	1.0639228159204754	3.2048626006727466E-4
56-57	0.0	0.0	0.0	1.2455011741243056	3.2048626006727466E-4
58-59	0.0	0.0	0.0	1.4526039737549223	3.2048626006727466E-4
60-61	0.0	0.0	0.0	1.6717021162623427	3.2048626006727466E-4
62-63	0.0	0.0	0.0	1.906343842383026	3.2048626006727466E-4
64-65	0.0	0.0	0.0	2.143869944844315	3.2048626006727466E-4
66-67	0.0	0.0	0.0	2.4117964582605564	3.2048626006727466E-4
68-69	0.0	0.0	0.0	2.7154114059228607	3.2048626006727466E-4
70-71	0.0	0.0	0.0	3.0509834120890167	3.2048626006727466E-4
72-73	0.0	0.0	0.0	3.391545847161934	4.1205376294363883E-4
74-75	0.0	0.0	0.0	3.725927475790697	4.1205376294363883E-4
76-77	0.0	0.0	0.0	4.107512152152226	4.1205376294363883E-4
78-79	0.0	0.0	0.0	4.5170935925182025	4.1205376294363883E-4
80-81	0.0	2.2891875719091045E-5	0.0	4.949681367981865	4.3494563866272986E-4
82-83	0.0	4.578375143818209E-5	0.0	5.420155197760625	4.5783751438182094E-4
84-85	0.0	4.578375143818209E-5	0.0	5.910293148782084	4.5783751438182094E-4
86-87	0.0	4.578375143818209E-5	0.0	6.404849231817327	4.5783751438182094E-4
88	0.0	4.578375143818209E-5	0.0	6.803740166222488	4.5783751438182094E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4230	0.0	42.362297	1
GGTATCA	2405	0.0	39.018833	1
CAACGCA	4700	0.0	36.998665	5
ATCAACG	4865	0.0	35.45645	3
TATCAAC	5250	0.0	33.841103	2
AACGCAG	5445	0.0	32.883606	6
TCAACGC	5295	0.0	32.75386	4
ACGCAGA	6165	0.0	28.20467	7
GTACATG	9175	0.0	27.445518	1
TACATGG	9340	0.0	26.822077	2
CGCAGAG	6650	0.0	25.86496	8
ACATGGG	9845	0.0	25.01656	3
GTACTAG	840	0.0	24.700722	1
CATGGGG	8090	0.0	23.587374	4
GCAGAGT	7485	0.0	22.540573	9
ATGGGGG	5425	0.0	22.351269	5
TGGGGGG	4765	0.0	21.79625	6
GAGTACG	210	9.237574E-7	20.20968	1
TATGTAA	590	0.0	19.915905	2
CGTTAAC	385	0.0	19.597267	1
>>END_MODULE