FastQCFastQC Report
Thu 26 May 2016
ERR580099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9500416
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227920.23990528414755732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175650.18488664075341543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100220.1054901174853817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150050.048.9619061
GTATCAA274150.041.70711
CAACGCA311000.035.2201585
ATCAACG319500.034.3710823
TATCAAC343450.032.9386442
AACGCAG356800.031.7064976
GTACTAG74300.030.765271
TCAACGC375200.029.457424
ACGCAGA370800.029.4460327
CGCAGAG375400.028.8977898
TAGGCAT95350.027.6590945
GTGGTAT45750.026.729791
GGGCACC81650.026.65560594
GCATCGT78150.026.572248
GTACATG407500.026.108281
GCAGAGT410450.025.952759
CTAGGCA105800.025.8163834
TAGTACT102400.025.1585454
TACATGG415350.025.131152
CTGTGCG99250.025.0921279
Produced by FastQC (version 0.11.5)