FastQCFastQC Report
Thu 26 May 2016
ERR580099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9500416
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199220.20969608067688827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162580.171129348441163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95440.10045875885855944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152750.047.9204141
GTATCAA274450.039.946481
CAACGCA311650.033.8878445
ATCAACG319600.033.060123
TATCAAC347700.031.3015882
GTACTAG74700.030.7752421
AACGCAG357900.030.3608286
TAGGCAT93200.028.4931565
TCAACGC377650.028.276734
GTGGTAT50250.028.2542691
ACGCAGA374300.028.0511597
CGCAGAG379900.027.501878
GGGCACC82750.025.50069894
GTACATG406700.025.3153041
CTAGGCA106450.025.0791594
GCAGAGT415150.024.870669
CTGTGCG95950.024.7837969
TACATGG412200.024.692782
ACATGGG401350.024.4641483
GCATCGT91550.023.6151948
Produced by FastQC (version 0.11.5)