FastQCFastQC Report
Thu 26 May 2016
ERR580098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441412
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70170.15799029677949264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59530.13403395136501634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67450.049.9853171
GTATCAA117750.040.133811
CAACGCA136500.032.9288565
ATCAACG139900.032.265563
TATCAAC147600.031.6031652
TCAACGC149750.030.3616774
AACGCAG150800.030.2911456
ACGCAGA166450.026.9339897
CGCAGAG171850.025.977398
GTACTAG20300.025.7117841
GTACATG198100.025.1846641
GTGGTAT29400.024.3109261
TACATGG202100.024.2208672
ACATGGG205000.023.1431773
GCAGAGT189400.022.7078029
CATGGGG159700.021.0801734
GAGTACT73150.020.43875312-13
TGGTATC33500.020.3532
CGTTAAC7100.019.868641
CAGAGTA184400.019.76986910-11
Produced by FastQC (version 0.11.5)