FastQCFastQC Report
Thu 26 May 2016
ERR580098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441412
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62390.14047334496326844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.13065664703026875No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63000.051.208031
GTATCAA114450.040.7982861
CAACGCA132550.033.5421525
ATCAACG135600.032.6158753
TATCAAC144150.031.8862722
AACGCAG146700.030.5627846
TCAACGC147350.030.0781354
ACGCAGA165600.026.4502757
GTACATG199000.025.8816571
GTACTAG19600.025.74841
CGCAGAG172050.025.3494178
TACATGG202900.025.0393562
ACATGGG206650.023.767313
GCAGAGT193050.022.0317279
CATGGGG161000.021.0474154
GAGTACT76350.020.40421112-13
GTGGTAT28750.020.3425941
CAGAGTA185800.019.1088310-11
AGAGTAC173550.018.62984310-11
GAGTACA150400.018.627781
Produced by FastQC (version 0.11.5)