FastQCFastQC Report
Thu 26 May 2016
ERR580097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6160119
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185780.30158508301544174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145510.23621296926244445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93210.15131201199197614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99750.054.4911881
GTATCAA185400.042.099771
CAACGCA218700.034.2580035
ATCAACG219650.034.087883
TATCAAC233450.033.005952
AACGCAG240250.031.9101416
TCAACGC239450.031.4659734
ACGCAGA259700.028.8523967
CGCAGAG264300.028.2262738
GTACATG253800.028.1600421
GTACTAG36100.027.8732721
TACATGG257500.027.2029652
ACATGGG254400.026.7711283
GTGGTAT38800.026.6607461
GCATCGT34800.025.2442558
CATGGGG190450.024.9265924
GCAGAGT292250.024.9183739
ATGGGGG146000.023.8876555
CTGTGCG46000.023.6958719
TAGTACT51000.023.1326854
Produced by FastQC (version 0.11.5)