FastQCFastQC Report
Thu 26 May 2016
ERR580097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6160119
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164650.2672837976019619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138560.2249307196825256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87690.14235114613857297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103250.053.874611
GTATCAA182000.039.709271
ATCAACG216000.032.400943
CAACGCA217400.032.1693575
TATCAAC233950.030.7102032
AACGCAG238200.030.1061616
TCAACGC239050.029.5711844
GTACTAG37700.028.9358371
GTACATG243350.027.3989771
ACGCAGA258200.026.9733317
GTGGTAT35250.026.5450421
CGCAGAG263300.026.3614168
ACATGGG243050.026.0681923
TACATGG251400.025.9788152
GCATCGT40750.025.37088
TAGGCAT48300.025.0083485
CTGTGCG43950.024.164329
GCAGAGT291000.023.447619
CATGGGG185900.023.235164
CGACGAG21950.023.12271924-25
Produced by FastQC (version 0.11.5)