FastQCFastQC Report
Thu 26 May 2016
ERR580096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12229317
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359470.29394119066502244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288130.2356059622953596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180430.14753890180457338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178900.049.5851361
GTATCAA317400.040.099691
CAACGCA363950.033.9311455
ATCAACG369950.033.4504173
TATCAAC400250.031.5294652
AACGCAG409050.030.9326136
GTACATG423850.030.050841
GTACTAG78300.029.9749811
TCAACGC416850.029.910484
TACATGG428850.028.9770832
ACATGGG425400.028.095123
ACGCAGA443900.027.9426047
CGCAGAG456200.027.1678378
CATGGGG333250.026.65974
GCATCGT81600.025.3432738
ATGGGGG252200.025.023785
TAGTACT105550.024.9975854
CTGTGCG103400.024.818529
GCAGAGT506350.024.1059869
GTGGTAT69900.023.6856671
Produced by FastQC (version 0.11.5)