FastQCFastQC Report
Thu 26 May 2016
ERR580096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12229317
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312860.25582786021492454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256540.20977459329903705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170730.1396071424103243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183850.046.613841
GTATCAA322300.039.7318341
CAACGCA372650.032.9817435
ATCAACG378450.032.4766773
TATCAAC412250.030.8068452
AACGCAG416200.030.4099716
TCAACGC421450.029.4528394
GTACATG411150.027.8527981
GTACTAG76000.027.587551
ACGCAGA458800.026.8590747
TACATGG419850.026.7115362
TAACGCC34900.026.3958384
CGCAGAG470000.026.2590248
ACATGGG412050.026.1896883
CTAACGC34150.026.1499733
CTGTGCG106100.025.9575639
TAGTACT109800.025.597864
TCTAACG35300.025.0325852
CATGGGG324600.024.2243544
CTAGTAC121700.023.9833263
Produced by FastQC (version 0.11.5)