FastQCFastQC Report
Thu 26 May 2016
ERR580095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9031139
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283000.3133602527876052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236890.2623035699040841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138040.15284893743745942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141550.053.0513341
GTATCAA256500.041.907341
CAACGCA310450.033.7259985
ATCAACG318950.032.8457683
TATCAAC329050.032.3816762
TCAACGC335800.031.546454
AACGCAG336250.031.4310556
ACGCAGA378400.027.5690387
GTGGTAT59600.027.1402241
GTACTAG42850.026.8854031
CGCAGAG392500.026.494128
GTACATG333550.026.235341
TACATGG339700.025.5527362
ACATGGG346850.024.1304343
GCAGAGT424250.023.9632579
TGGTATC64950.023.8910962
TAGTACT55200.023.6796744
CATGGGG261700.022.242674
CTAGTAC60700.022.1546133
GAGTACT243250.021.34671812-13
Produced by FastQC (version 0.11.5)