FastQCFastQC Report
Thu 26 May 2016
ERR580095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9031139
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258240.2859439988688027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227200.2515740262662329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132950.1472128820074633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148200.052.9340321
GTATCAA262900.040.569621
CAACGCA322300.032.226895
ATCAACG326550.031.8223863
TATCAAC340600.031.171182
AACGCAG344350.030.5430776
TCAACGC345200.030.2527264
GTGGTAT58300.029.695351
GTACTAG42200.026.8280871
ACGCAGA384750.026.8229477
CGCAGAG400400.025.5750188
GTACATG328750.025.4553321
TACATGG334400.024.778082
TGGTATC67800.024.1229922
ACATGGG339850.023.4824853
GCAGAGT433550.023.0992079
GAGTACT249700.021.72806212-13
TAGTACT59200.021.6736584
CATGGGG256200.021.4817184
CAGAGTA424950.020.34253910-11
Produced by FastQC (version 0.11.5)