FastQCFastQC Report
Thu 26 May 2016
ERR580094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6616649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225350.3405802544460194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184210.27840376601509315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111290.16819692264165742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115650.053.6851121
GTATCAA213150.042.1256071
CAACGCA249750.034.9366345
ATCAACG252100.034.5723653
TATCAAC271050.033.0827752
AACGCAG276000.032.2245836
GTACTAG45800.031.6308461
TCAACGC284450.030.8399964
ACGCAGA297700.029.1505647
GTGGTAT35500.028.8837591
CGCAGAG302600.028.5696248
GTACATG292600.028.5492171
TACATGG298700.027.8164652
GCATCGT48550.027.581088
ACATGGG294400.027.3534263
TAGTACT61900.026.8097464
TAGGCAT60250.026.6856425
CTGTGCG60600.026.4435359
CTAACGC22100.026.3767683
GCAGAGT329250.025.9338449
Produced by FastQC (version 0.11.5)