FastQCFastQC Report
Thu 26 May 2016
ERR580094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6616649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196290.29666074171381923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174240.2633357157074525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109740.16585434711740035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117650.052.027071
GTATCAA210400.039.4374921
GTACTAG47650.033.7422071
CAACGCA245950.032.8109555
ATCAACG249850.032.318593
GTGGTAT38400.031.5863021
TATCAAC269450.030.6900752
AACGCAG273100.030.3737726
CTGTGCG60450.029.6199769
TCAACGC278550.029.156984
TAGGCAT60700.029.036085
TAGTACT63050.028.8488064
ACGCAGA297850.027.0135657
GGGCACC59600.026.73145594
CTAGGCA68250.026.7196674
CGCAGAG302200.026.5156568
GCATCGT56450.026.308478
GTACATG285600.026.0575161
CTAGTAC73600.025.8634093
TACATGG291050.025.4080542
Produced by FastQC (version 0.11.5)