FastQCFastQC Report
Thu 26 May 2016
ERR580093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989022
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.5576215695909696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33450.33821290122969966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31210.3155642644956331No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26870.27168253082337906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.2696603311149803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.12729747164370459No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.11455761348079213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.050.5747451
GTATCAA28450.041.8213421
GTGGTAT5650.040.785651
CAACGCA33900.033.849315
TATCAAC34850.033.737972
ATCAACG34350.033.544483
AACGCAG39000.030.0136386
TGGTATC7900.029.1709562
CCGTATC4550.027.88857894
TCAACGC42050.027.8479394
ACGCAGA41350.027.7394717
CGCAGAG42800.026.577348
GCAGAGT44850.025.3638299
GCATATA2151.8644641E-924.0610141
GTACATG20800.023.0619771
CATGGGG17400.022.9736184
ACATGGG21300.022.0801543
TACATGG21550.021.8240032
GTACTAG4800.021.5546591
CGTGCAC1354.0585492E-420.9025463
Produced by FastQC (version 0.11.5)