FastQCFastQC Report
Thu 26 May 2016
ERR580093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989022
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.50635880698306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.30899211544333693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28640.2895789982427085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.2738058405171978No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.25075276384145145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.12112976253308824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.049.8294871
GTATCAA27550.045.7261621
GTGGTAT5350.036.9017641
TATCAAC35000.035.9875642
ATCAACG34450.035.4707073
CAACGCA34950.034.694315
AACGCAG38950.031.734676
TCAACGC40900.030.1067394
ACGCAGA41900.028.6030187
TAGACGG1652.9649527E-928.484055
CGCAGAG43300.027.6782078
TGGTATC8050.026.8563922
CGTAGAC1807.497874E-926.110383
GCAGAGT46100.025.6912579
GTACATG19750.024.752431
TACATGG19800.023.7367122
ACATGGG20000.022.7943653
CTGTGCG4300.021.8598549
GTACTAG4750.021.7711141
GAGTACT36100.021.28610812-13
Produced by FastQC (version 0.11.5)