FastQCFastQC Report
Thu 26 May 2016
ERR580092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10002281
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271700.27163803936322123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226760.2267082878395438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134860.13482924544911307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176150.047.138911
GTATCAA306650.038.577951
CAACGCA335950.033.3205955
ATCAACG344300.032.5147823
GTGGTAT51950.032.4922941
GTACTAG92050.031.5161291
TATCAAC382500.030.7312372
GGGCACC97150.030.37731494
AACGCAG387850.030.000176
TAGTACT121900.027.4617524
TAGGCAT113400.027.4456145
ACGCAGA407550.027.4310057
CTGTGCG120050.027.3655389
TCAACGC419450.027.0812474
CGCAGAG414550.026.8880588
GCATCGT93950.026.6094868
CTAGGCA125950.025.4214824
GTACATG428300.025.4031011
CTAACGC47600.025.286663
TACATGG430200.024.9411642
Produced by FastQC (version 0.11.5)