FastQCFastQC Report
Thu 26 May 2016
ERR580092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10002281
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236900.23684597543300376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210250.2102020529117308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123520.12349183151323183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183550.046.8158571
GTATCAA313500.037.6098371
GTGGTAT53100.034.9057541
CAACGCA354000.031.7175965
ATCAACG358050.031.3071063
GGGCACC99950.031.03383894
GTACTAG93750.030.1338441
TATCAAC401150.029.219932
TAGGCAT112550.028.7714085
AACGCAG407850.028.3346086
TAGTACT127250.027.036164
CTGTGCG126450.026.907719
TCAACGC432300.026.3752924
ACGCAGA429000.026.0065387
CTAGGCA129700.025.8007134
CTAACGC45050.025.7691673
CGCAGAG433400.025.5690178
TCTAACG46400.025.120212
GTACATG439000.024.9672431
TACCCCG8100.024.9500145
Produced by FastQC (version 0.11.5)