FastQCFastQC Report
Thu 26 May 2016
ERR580091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8667826
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166350.19191663515165164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120950.1395390262794846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106700.041.741791
GTATCAA185050.039.1142541
CAACGCA199950.034.362065
ATCAACG206750.033.2129823
GTACTAG55100.031.6670651
TATCAAC229200.031.3344782
AACGCAG229250.031.0225036
GTACATG294950.029.7542321
TCAACGC237550.029.3212874
TACATGG299100.028.8076022
ACATGGG295550.027.9920983
ACGCAGA249800.027.6043437
GCATCGT57700.026.5568478
CGCAGAG259850.026.4459638
CATGGGG243200.026.2228054
CTGTGCG80650.025.6435439
TAGTACT81100.025.458144
CTAACGC25350.025.2325733
TCTAACG25900.024.8781932
GAGTACA208200.024.5320951
Produced by FastQC (version 0.11.5)