FastQCFastQC Report
Thu 26 May 2016
ERR580091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8667826
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145690.16808136203933952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109890.1267791946908025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105550.042.6228681
GTATCAA189400.039.3403471
CAACGCA211150.033.1662525
ATCAACG219050.031.9706733
TATCAAC241600.030.4469432
AACGCAG242950.029.5977176
GTACTAG58200.029.0782621
GTACATG291550.028.523561
TCAACGC249300.028.3172724
TACATGG298400.027.5497262
ACATGGG292200.026.8624113
ACGCAGA265650.026.4493547
CTGTGCG84750.026.0087439
CTAACGC25050.025.8928663
TAGTACT84550.025.5710164
TCTAACG26200.025.4749492
CGCAGAG276650.025.3807038
CATGGGG236950.025.330164
ATGGGGG174700.025.020235
TAACGCC26500.024.8305244
Produced by FastQC (version 0.11.5)