FastQCFastQC Report
Thu 26 May 2016
ERR580090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181498
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35280.110891158818896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.049.9830971
GTATCAA65750.040.7635731
CAACGCA77250.032.9263885
ATCAACG78700.032.3825343
TATCAAC82500.032.203372
AACGCAG86350.030.2025596
TCAACGC85550.030.0085684
GTACTAG14750.026.778141
ACGCAGA96050.026.0765277
CGCAGAG99500.025.1251378
GTACATG122550.024.939811
TACATGG123550.024.3581332
ACATGGG127000.023.2880543
GCAGAGT113400.021.634449
CATGGGG97100.021.0640854
GCATCGT15850.020.160428
TAGGCAT22200.019.907665
GTATAAG17550.019.558651
GTGGTAT16550.019.3198621
TACCCCG3204.5474735E-1019.1002335
Produced by FastQC (version 0.11.5)