FastQCFastQC Report
Thu 26 May 2016
ERR580090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181498
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.043.199141
GTATCAA67800.039.163281
CAACGCA76300.033.5065275
ATCAACG77550.033.1498073
TATCAAC81950.032.2291762
AACGCAG85800.030.3986236
TCAACGC84850.030.3522244
ACGCAGA97400.026.1027647
CGCAGAG102850.024.3997368
GTACATG125350.023.21461
TACATGG123900.022.6825432
GTGGTAT18850.021.5172841
ACATGGG128050.021.214063
GCAGAGT117900.020.846649
GTACTAG14350.020.0483341
CATGGGG97100.019.4566764
GTATATA13000.019.2279451
GTGTAGC20700.018.4550421
GAGTACT43050.017.90215312-13
AGAGTAC102800.017.85099610-11
Produced by FastQC (version 0.11.5)