FastQCFastQC Report
Thu 26 May 2016
ERR580089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808447
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.1633287008798813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38770.13804782500791363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.048.225741
GTATCAA66600.039.042211
CAACGCA75950.032.4988945
ATCAACG77750.031.6300813
TATCAAC80900.031.3865972
TCAACGC81500.030.2313234
AACGCAG83650.030.2207896
GTACATG98350.027.2032871
GTACTAG11300.026.630851
ACGCAGA93350.026.2742427
TACATGG100600.026.081662
CGCAGAG97450.025.0232728
ACATGGG102950.024.5728153
GTGGTAT17450.024.5205081
CATGGGG80950.022.5371174
GCAGAGT108850.022.0211269
GCATCGT11250.021.7174788
ATGGGGG53600.020.7006655
CGTTAAC2551.3415047E-820.2831781
TGGTATC19500.020.2555332
Produced by FastQC (version 0.11.5)