FastQCFastQC Report
Thu 26 May 2016
ERR580089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808447
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.1443146336747676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.12772183345457472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.048.242321
GTATCAA64500.039.3416061
CAACGCA75200.032.1856165
ATCAACG75850.031.9109363
TATCAAC81250.030.7156012
TCAACGC81050.029.979044
AACGCAG83750.029.2900096
GTACTAG11850.027.0657521
ACGCAGA93800.025.6507997
CGCAGAG98550.024.1760378
GTGGTAT18550.023.9008581
GTACATG97650.023.8607581
TACATGG101300.022.733932
ACATGGG102600.022.0794163
GCAGAGT112200.020.7741189
CATGGGG82100.020.4936854
TGGTATC20500.019.9458852
GCATCGT14700.019.5005258
ATAACGC2902.7866918E-919.4478283
TATACCG4600.019.4119075
Produced by FastQC (version 0.11.5)