FastQCFastQC Report
Thu 26 May 2016
ERR580088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708894
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61580.16603332422010444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.13017357735217022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.044.9229471
GTATCAA81250.038.4320641
CAACGCA93900.032.5007135
ATCAACG95300.032.026293
TCAACGC100150.030.6619324
TATCAAC101350.030.3001162
AACGCAG104550.029.8910986
GTACATG123950.027.3171221
TACATGG128400.025.9678542
ACGCAGA119000.025.7470427
CGCAGAG123450.024.7047398
ACATGGG130150.024.6430843
TACCTGG26100.024.3247362
CATGGGG95350.023.6732444
ATGGGGG62900.022.2781665
GCAGAGT140950.021.1387929
TATCACG3351.8189894E-1221.0572322
TGGGGGG59900.019.8489696
TAACGCA6250.019.562784
GTACCTG33900.019.559981
Produced by FastQC (version 0.11.5)