FastQCFastQC Report
Thu 26 May 2016
ERR580088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708894
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.15457438255177958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.12095249958613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.044.278161
GTATCAA80650.035.9617541
CAACGCA92950.029.8838045
ATCAACG93750.029.7298643
TACCTGG26400.029.3753932
TCAACGC97750.028.512534
TATCAAC100450.028.0740432
AACGCAG102600.027.894276
TATCACG3350.026.6570722
TAACGCA5150.026.4660154
ATAACGC5300.025.7176693
GTACATG122050.025.6953161
GTATAAC10150.024.6252081
TACATGG124250.024.3987162
ACGCAGA115750.023.9132947
GTACCTG34550.023.4774271
TATAACG7050.023.3336452
ACATGGG125950.022.95023
CGCAGAG121950.022.65938
ACCTGGG35350.021.8053933
Produced by FastQC (version 0.11.5)