FastQCFastQC Report
Thu 26 May 2016
ERR580087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225490
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125400.040.5680731
GGTATCA73500.038.1253281
CAACGCA130450.036.5432325
ATCAACG140000.033.985583
TATCAAC150650.033.644392
AACGCAG154150.031.9196596
TCAACGC161550.029.9177114
ACGCAGA165600.028.4926367
GTACTAG42600.027.4817521
CGCAGAG171600.027.3310228
GTATAAG42000.026.4190641
GTGATCG33350.025.7868238
GTGGTAT23600.024.9030591
TGATCGC36150.023.6633599
GCAGAGT195200.023.5489949
TAGGCAT59050.023.406725
GCATCGT46150.023.1151528
CGTTAAC10850.022.9668181
GTACATG233250.022.95921
AAGCTAA31150.022.7841179
Produced by FastQC (version 0.11.5)